scholarly journals Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India

2013 ◽  
Vol 24 (2) ◽  
pp. 188-198 ◽  
Author(s):  
V. Venkataravanappa ◽  
C. N. Lakshminarayana Reddy ◽  
A. Devaraju ◽  
Salil Jalali ◽  
M. Krishna Reddy
Keyword(s):  
Yellow Vein ◽  
Cotton Leaf ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals Association of a Begomovirus and Nanovirus-like Molecule with Ageratum Yellow Vein Disease in Pakistan

Plant Disease ◽  
2000 ◽  
Vol 84 (1) ◽  
pp. 101-101 ◽  
Author(s):  
S. Mansoor ◽  
S. H. Khan ◽  
M. Hussain ◽  
Y. Zafar ◽  
M. S. Pinner ◽  
...  
Keyword(s):  
Full Length ◽  
Yellow Vein ◽  
Cotton Leaf ◽  
Degenerate Primers ◽  
Total Dna ◽  
Leaf Curl Virus ◽  
Leaf Curl Disease ◽  
Vein Disease ◽  
Full Length Clone ◽  
Leaf Curl

Whitefly-transmitted geminiviruses (begomoviruses) cause heavy losses to many food and fiber crops in Pakistan. Many weeds also show symptoms typical of begomoviruses. Ageratum (Ageratum conyzoides) is a common perennial weed in Pakistan, growing along irrigation canals, that often shows symptoms, such as yellow vein and mosaic, suggesting infection by a begomovirus. To confirm this, symptomatic and asymptomatic ageratum plants were collected from three locations in the Punjab Province of Pakistan, and total DNA was isolated, subjected to agarose gel electrophoresis, transferred to a nylon membrane, and Southern blotted. Total DNA isolated from cotton infected with Cotton leaf curl virus (CLCuV), tomato infected with Tomato leaf curl virus from Pakistan (TLCV-Pak), tobacco infected with African cassava mosaic virus (ACMV) from Nigeria, and healthy tobacco were included as controls. A full-length clone of CLCuV DNA A was labeled with [32P]dCTP by oligo-labeling and hybridized at medium stringency. The probe detected characteristic geminivirus DNA forms in symptomatic ageratum and plants infected with CLCuV, TLCV-Pak, and ACMV, while no signal was detected in asymptomatic ageratum from the field or healthy tobacco. To confirm infection by a begomovirus, degenerate primers WTGF (5′-GATTGTACGCGTCCDCCTTTAATTT GAAYBGG-3′), designed in the rep gene of begomoviruses, and WTGR (5′-TANACGCGTGGC TTCKRTACATGGCCTDT-3′), designed in the coat protein gene of DNA A of begomoviruses, were used in polymerase chain reaction (PCR). Degenerate primers (PBLv2040 and PCRc1) also were used in PCR (2). A product of expected size (≈1.4 kb) was obtained with DNA A primers from symptomatic ageratum, while no product was obtained with DNA B primers in the same sample. Previously we were unable to detect a DNA component equivalent to begomovirus DNA B in cotton showing symptoms of cotton leaf curl disease (1). We recently reported a novel circular DNA molecule that was approximately half as long as the full-length DNA A (CLCuV DNA-1) associated with CLCuV that share homology to plant nanoviruses (1). The supercoiled replicative form of viral DNA isolated from infected ageratum plants indicated the presence of smaller molecules, as was found in cotton leaf curl disease, suggesting that a nanovirus-like molecule might be associated with ageratum yellow vein disease. A duplicate blot of samples used in Southern hybridization with the DNA A probe was prepared, and a probe of the full-length clone of the nanovirus-like molecule (CLCuV DNA-1) was prepared as described for DNA A. The probe detected characteristic nanovirus DNA forms in ageratum with yellow vein symptoms and cotton infected with CLCuV, while no signal was detected in plants infected with TLCV-Pak or ACMV, healthy tobacco, or asymptomatic ageratum. Abutting primers PB2-F and PB2R (1), designed based on the CLCuV DNA-1 sequence, were unable to amplify a PCR product from ageratum with yellow vein symptoms, suggesting the nanovirus-like molecule associated with ageratum yellow vein disease is distinct from CLCuV DNA-1. Our results show that yellow vein disease of ageratum in Pakistan is associated with a begomovirus infection and single-stranded circular DNA molecule with similarity to CLCuV DNA-1. References: (1) S. Mansoor et al. Virology 259:190, 1999. (2) M. R. Rojas et al., Plant Dis. 77:340, 1993.

scholarly journals Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus

2021 ◽  
Vol 9 (2) ◽  
pp. 304
Author(s):  
Yao Chi ◽  
Li-Long Pan ◽  
Shu-Sheng Liu ◽  
Shahid Mansoor ◽  
Xiao-Wei Wang
Keyword(s):  
Coat Protein ◽  
Molecular Mechanisms ◽  
Cotton Leaf ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Vacuolar Protein ◽  
Leaf Curl Disease ◽  
Two Hybrid ◽  
Asia Ii 1 ◽  
Leaf Curl

Cotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the Bemisia tabaci complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms underlying the transmission are poorly known. In this study, we attempted to identify the whitefly proteins involved in CLCuMuV transmission. First, using a yeast two-hybrid system, we identified 54 candidate proteins of Asia II 1 that putatively can interact with the coat protein of CLCuMuV. Second, we examined interactions between the CLCuMuV coat protein and several whitefly proteins, including vacuolar protein sorting-associated protein (Vps) twenty associated 1 (Vta1). Third, using RNA interference, we found that Vta1 positively regulated CLCuMuV acquisition and transmission by the Asia II 1 whitefly. In addition, we showed that the interaction between the CLCuMuV coat protein and Vta1 from the whitefly Middle East-Asia Minor (MEAM1), a poor vector of CLCuMuV, was much weaker than that between Asia II 1 Vta1 and the CLCuMuV coat protein. Silencing of Vta1 in MEAM1 did not affect the quantity of CLCuMuV acquired by the whitefly. Taken together, our results suggest that Vta1 may play an important role in the transmission of CLCuMuV by the whitefly.

scholarly journals Engineering broad-spectrum resistance to cotton leaf curl disease by CRISPR-Cas9 based multiplex editing in plants

2021 ◽  
pp. 1-12
Author(s):  
Muhammad Salman Mubarik ◽  
Xiukang Wang ◽  
Sultan Habibullah Khan ◽  
Aftab Ahmad ◽  
Zulqurnain Khan ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Cotton Leaf ◽  
Broad Spectrum Resistance ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals Diversity of alphasatellites associated with cotton leaf curl disease in Pakistan

2016 ◽  
Vol 6 ◽  
pp. 41-52 ◽  
Author(s):  
Komal Siddiqui ◽  
Shahid Mansoor ◽  
Rob W. Briddon ◽  
Imran Amin
Keyword(s):  
Cotton Leaf ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

Cotton Leaf Curl Disease

2008 ◽  
pp. 563-569 ◽  
Author(s):  
S. Mansoor ◽  
I. Amin ◽  
R.W. Briddon
Keyword(s):  
Cotton Leaf ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

Appearance of Cotton leaf curl disease begomovirus avirulent strains associated with a lower disease incidence in Delhi

2020 ◽  
Vol 28 (1) ◽  
pp. 67
Author(s):  
Manisha Duhan ◽  
Subhankar Gupta ◽  
U.K. Bhattacharyya ◽  
S. Palchoudhury ◽  
K.K. Biswas
Keyword(s):  
Disease Incidence ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

Molecular Screening of Cotton Germplasm for Cotton Leaf Curl Disease Caused by Viral Strains

2017 ◽  
Vol 19 (01) ◽  
pp. 125-130
Author(s):  
Huma Saleem ◽  
Asif Ali Khan ◽  
Muhammad Tehseen Azhar ◽  
Muhammad Shah Nawaz-ul-Rehman
Keyword(s):  
Cotton Leaf ◽  
Molecular Screening ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Virus Genes ◽  
2011 ◽  
Vol 42 (2) ◽  
pp. 286-296 ◽  
Author(s):  
Jamil A. Hashmi ◽  
Yusuf Zafar ◽  
Muhammad Arshad ◽  
Shahid Mansoor ◽  
Shaheen Asad
Keyword(s):  
Gossypium Hirsutum ◽  
Dna Sequences ◽  
Cotton Leaf ◽  
Gossypium Hirsutum L ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl
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