Ectopic expression of the PttKN1 gene induced altered leaf morphology and hormonal levels in transgenic tobacco

2014 ◽  
Vol 24 (2) ◽  
pp. 197-203
Author(s):  
Quanle Xu ◽  
Nan Gao ◽  
Meiyu Ruan ◽  
Weiqiao Ding ◽  
Xin Hu ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Leaf Morphology ◽  
Ectopic Expression ◽  
Pttkn1 Gene ◽  
Hormonal Levels

Transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth Galleria mellonella, confers resistance to pathogenic fungi in tobacco

2006 ◽  
Vol 387 (5) ◽  
pp. 549-557 ◽  
Author(s):  
Gregor Langen ◽  
Jafargholi Imani ◽  
Boran Altincicek ◽  
Gernot Kieseritzky ◽  
Karl-Heinz Kogel ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Fungal Pathogens ◽  
Galleria Mellonella ◽  
Ectopic Expression ◽  
Pathogenic Fungi ◽  
Transgenic Expression ◽  
Greater Wax Moth ◽  
Insect Defensin ◽  
Wax Moth

Abstract A cDNA encoding gallerimycin, a novel antifungal peptide from the greater wax moth Galleria mellonella, was isolated from a cDNA library of genes expressed during innate immune response in the caterpillars. Upon ectopic expression of gallerimycin in tobacco, using Agrobacterium tumefaciens as a vector, gallerimycin conferred resistance to the fungal pathogens Erysiphe cichoracearum and Sclerotinia minor. Quantification of gallerimycin mRNA in transgenic tobacco by real-time PCR confirmed transgenic expression under control of the inducible mannopine synthase promoter. Leaf sap and intercellular washing fluid from transgenic tobacco inhibited in vitro germination and growth of the fungal pathogens, demonstrating that gallerimycin is secreted into intercellular spaces. The feasibility of the use of gallerimycin to counteract fungal diseases in crop plants is discussed.

scholarly journals Ectopic Expression of the Rice Lumazine Synthase Gene Contributes to Defense Responses in Transgenic Tobacco

2010 ◽  
Vol 100 (6) ◽  
pp. 573-581 ◽  
Author(s):  
Tingquan Wu ◽  
An Guo ◽  
Yanying Zhao ◽  
Xiaomeng Wang ◽  
Ying Wang ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Transgenic Tobacco ◽  
Plant Defenses ◽  
Higher Plants ◽  
Synergistic Effects ◽  
Ectopic Expression ◽  
Defense Responses ◽  
Flavin Mononucleotide ◽  
Lumazine Synthase ◽  
Ethylene Content

Lumazine synthase (LS) catalyzes the penultimate reaction in the multistep riboflavin biosynthesis pathway, which is involved in plant defenses. Plant defenses are often subject to synergistic effects of jasmonic acid and ethylene whereas LS is a regulator of jasmonic acid signal transduction. However, little is known about whether the enzyme contributes to defense responses. To study the role of LS in plant pathogen defenses, we generated transgenic tobacco expressing the rice (Oryza sativa) LS gene, OsLS. OsLS was cloned and found to have strong identity with its homologues in higher plants and less homology to microbial orthologues. The OsLS protein localized to chloroplasts in three OsLS-expressing transgenic tobacco (LSETT) lines characterized as enhanced in growth and defense. Compared with control plants, LSETT had higher content of both riboflavin and the cofactors flavin mononucleotide and flavin adenine dinucleotide. In LSETT, jasmonic acid and ethylene were elevated, the expression of defense-related genes was induced, levels of resistance to pathogens were enhanced, and resistance was effective to viral, bacterial, and oomycete pathogens. Extents of OsLS expression correlated with increases in flavin, jasmonic acid, and ethylene content, and correlated with increases in resistance levels, suggesting a role for OsLS in defense responses.

Ectopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco

2012 ◽  
Vol 39 (12) ◽  
pp. 10201-10209 ◽  
Author(s):  
Luciana C. Cidade ◽  
Tahise M. de Oliveira ◽  
Amanda F. S. Mendes ◽  
Amanda F. Macedo ◽  
Eny I. S. Floh ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Ectopic Expression ◽  
Phytoene Synthase ◽  
Citrus Paradisi

scholarly journals Transgenic Plants Producing the Bacterial Pheromone N-Acyl-Homoserine Lactone Exhibit Enhanced Resistance to the Bacterial Phytopathogen Erwinia carotovora

2001 ◽  
Vol 14 (9) ◽  
pp. 1035-1042 ◽  
Author(s):  
Andres Mäe ◽  
Marcos Montesano ◽  
Viia Koiv ◽  
E. Tapio Palva
Keyword(s):  
Quorum Sensing ◽  
Transgenic Tobacco ◽  
Plant Cell Wall ◽  
Ectopic Expression ◽  
Erwinia Carotovora ◽  
Homoserine Lactone ◽  
Defense Responses ◽  
Dependent Manner ◽  
Wild Type ◽  
Enhanced Resistance

Bacterial pheromones, mainly different homoserine lactones, are central to a number of bacterial signaling processes, including those involved in plant pathogenicity. We previously demonstrated that N-oxoacyl-homoserine lactone (OHL) is essential for quorum sensing in the soft-rot phytopathogen Erwinia carotovora. In this pathogen, OHL controls the coordinate activation of genes encoding the main virulence determinants, extracellular plant cell wall degrading enzymes (PCWDEs), in a cell density-dependent manner. We suggest that E. carotovora employ quorum sensing to avoid the premature production of PCWDEs and subsequent activation of plant defense responses. To test whether modulating this sensory system would affect the outcome of a plant-pathogen interaction, we generated transgenic tobacco, producing OHL. This was accomplished by ectopic expression in tobacco of the E. carotovora gene expI, which is responsible for OHL biosynthesis. We show that expI-positive transgenic tobacco lines produced the active pheromone and partially complemented the avirulent phenotype of expI mutants. The OHL-producing tobacco lines exhibited enhanced resistance to infection by wild-type E. carotovora. The results were confirmed by exogenous addition of OHL to wild-type plants, which also resulted in increased resistance to E. carotovora.

scholarly journals Ectopic Expression of Xylella fastidiosa rpfF Conferring Production of Diffusible Signal Factor in Transgenic Tobacco and Citrus Alters Pathogen Behavior and Reduces Disease Severity

2017 ◽  
Vol 30 (11) ◽  
pp. 866-875 ◽  
Author(s):  
R. Caserta ◽  
R. R. Souza-Neto ◽  
M. A. Takita ◽  
S. E. Lindow ◽  
A. A. De Souza
Keyword(s):  
Transgenic Tobacco ◽  
Disease Severity ◽  
Host Plants ◽  
Xylella Fastidiosa ◽  
Ectopic Expression ◽  
Signal Molecules ◽  
Insect Vector ◽  
Expression Of Genes ◽  
Diffusible Signal Factor ◽  
Population Sizes

The pathogenicity of Xylella fastidiosa is associated with its ability to colonize the xylem of host plants. Expression of genes contributing to xylem colonization are suppressed, while those necessary for insect vector acquisition are increased with increasing concentrations of diffusible signal factor (DSF), whose production is dependent on RpfF. We previously demonstrated that transgenic citrus plants ectopically expressing rpfF from a citrus strain of X. fastidiosa subsp. pauca exhibited less susceptibility to Xanthomonas citri subsp. citri, another pathogen whose virulence is modulated by DSF accumulation. Here, we demonstrate that ectopic expression of rpfF in both transgenic tobacco and sweet orange also confers a reduction in disease severity incited by X. fastidiosa and reduces its colonization of those plants. Decreased disease severity in the transgenic plants was generally associated with increased expression of genes conferring adhesiveness to the pathogen and decreased expression of genes necessary for active motility, accounting for the reduced population sizes achieved in the plants, apparently by limiting pathogen dispersal through the plant. Plant-derived DSF signal molecules in a host plant can, therefore, be exploited to interfere with more than one pathogen whose virulence is controlled by DSF signaling.

Ectopic Expression of a c1-I Allele From Maize Inhibits Pigment Formation in the Flower of Transgenic Tobacco

2004 ◽  
Vol 26 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Bojun Chen ◽  
Xiqing Wang ◽  
Yuanlei Hu ◽  
Yong Wang ◽  
Zhongping Lin
Keyword(s):  
Transgenic Tobacco ◽  
Ectopic Expression ◽  
Pigment Formation
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