Insecticidal activity of Callistemon speciosus essential oil on Anticarsia gemmatalis and Spodoptera frugiperda

Author(s):  
Wendel P. Silvestre ◽  
Camila B. Vicenço ◽  
Rafaela A. Thomazoni ◽  
Gabriel F. Pauletti
2018 ◽  
Vol 42 (4) ◽  
pp. 408-419 ◽  
Author(s):  
Ellison Rosario de Oliveira ◽  
Dejane Santos Alves ◽  
Geraldo Andrade Carvalho ◽  
Bárbara Maria Ribeiro Guimarães de Oliveira ◽  
Smail Aazza ◽  
...  

ABSTRACT Fall armyworm (FAW) (Spodoptera frugiperda) is a polyphagous insect responsible for damage to several crops. Synthetic chemical insecticides and genetically modified plants are the most commonly used methods for FAW control. However, the selection of resistant populations has been reported in several studies, justifying the search for new molecules to be used in the control of S. frugiperda. The aim of the present study was to evaluate the toxicity of lemongrass (Cymbopogon flexuosus) essential oil (LEO) and its major component (citral) in relation to FAW. Additionally, the anticholinesterase activity of LEO and citral was evaluated using acetylcholinesterase (AChE) from Electrophorus electricus. The LEO was toxic to FAW when added to an artificial diet (LC50 = 1.35 mg mL-1) at the highest concentrations tested, and the median lethal time (LT50) was 18.85 h. Major components of LEO were identified by gas chromatography-mass spectrometry, and citral, the most abundant component, was used in FAW bioassays. The insecticidal activity of citral was statistically similar to that of LEO, demonstrating that citral was responsible for the insecticidal activity of LEO. Inhibition of AChE was measured, and the mean inhibitory concentration (IC50) values for LEO and citral were 650- and 405-fold higher, respectively, than that verified for the positive control (methomyl insecticide), suggesting selectivity for non-target organisms. Based on these results, citral and C. flexuosus have the potential to be applied in the development of new products for the control of S. frugiperda.


Author(s):  
Camila Bonatto Vicenço ◽  
Wendel Paulo Silvestre ◽  
Iriane Viganó Menegol ◽  
Marina Cichin Carraro ◽  
Gabriel Fernandes Pauletti

2008 ◽  
Author(s):  
◽  
Vanesa Eva Dikgolz

En los últimos años el cultivo de soja se ha convertido en uno de los más importantes de nuestro país. Existe una gran diversidad de insectos fitófagos que atacan a los cultivos de soja y bajo determinadas condiciones pueden alcanzar niveles de densidad altos, convirtiéndose en plagas. Entre las principales especies de lépidopteros defoliadores considerados plaga se encuentran: la “oruga de las leguminosas” Anticarsia gemmatalis (Hubner), la “oruga medidora” Rachiplusia nu Guenneé, la “oruga de la alfalfa” Colias lesbias (Fabricius), la “oruga militar tardía” Spodoptera frugiperda (J. E. Smith), orugas del género Helicoverpa y la “gata peluda norteamericana” Spilosoma virginica (Fabricius). De menor importancia son la “oruguita de la verdolaga” Loxostege bifidalis Fabricius, el barrenador menor del tallo” Elasmopalpus lignosellus Séller, orugas del género Agrotis, Eulia loxonepes (Meyrick) y Prodenia ornithogalli (Guenneé). Estos insectos provocan daños en las hojas de la plantas, sobre todo antes de la formación de frutos. Los enemigos naturales de estos insectos están conformados por depredadores, parasitoides y patógenos. Entre los patógenos se destacan los hongos, los cuales se encuentran infectando gran diversidad de insectos y hábitats. Estos organismos son una herramienta útil dentro del manejo integrado de plagas. El principal hongo entomopatógeno relacionado con larvas de lépidopteros defoliadores, en particular de la Familia Noctuidae, es Nomuraea rileyi (Farlow) Samson (Ascomycota: Sordariomycetes: Hypocreales). Hasta el presente han sido realizados varios estudios a nivel mundial acerca del hongo N. rileyi, sobre su virulencia, rango hospedador, citología e inmunidad. En nuestro país se ha encontrado una importante acción del hongo N. rileyi en el control natural de lepidópteros defoliadores de soja, cuando se presentaron condiciones de humedad favorables para su dispersión, llegando a observarse un control del 60 al 90 % de las poblaciones de A. gemmatalis en la provincia de Santa Fe, siendo citada previamente la existencia de epizootias y control natural de esta especie plaga en la provincia de Córdoba. Hasta el presente hay pocos estudios de caracterización de poblaciones de N.rileyi de nuestro país y sobre todo de la región pampeana donde se concentra el 83,68 % del área sojera. Tampoco se han realizado hasta el momento estudios sobre el ciclo parasexual en este hongo por lo que resulta de importancia abordar estas temáticas. El objetivo de este trabajo fue evaluar las diferencias morfológicas, y genéticas de aislamientos nativos del hongo Nomuraea rileyi y evaluar su patogenicidad mediante bioensayos, así como también intentar determinar la existencia de un ciclo parasexual.


2013 ◽  
Vol 37 (2) ◽  
pp. 138-144 ◽  
Author(s):  
Patrícia Fontes Pinheiro ◽  
Vagner Tebaldi de Queiroz ◽  
Vando Miossi Rondelli ◽  
Adilson Vidal Costa ◽  
Tiago de Paula Marcelino ◽  
...  

The thrips, Frankliniella schultzei, and green peach aphid, Myzus persicae, cause direct damage to plants of economic importance and transmit phytoviruses, causing large economic losses. Chemical constituents of essential oils present a wide range of biological activities. The aim of this work was to evaluate insecticidal activity of essential oil from citronella grass, Cymbopogon winterianus, on F. schultzei and M. persicae. This essential oil was obtained by steam distillation and components were identified by GC/FID and GC/MS. A Potter spray tower was used to spray insects with the essential oil. The major constituents are geraniol (28.62%), citronellal (23.62%) and citronellol (17.10%). Essential oil of C. winterianus at 1% (w v-1) causes mortality in F. schultzei and M. persicae at 34.3% and 96.9%, respectively. The LC50 value for M. persicae was 0.36% and LC90 0.66%. Thus, citronella grass essential oil at 1% (w v-1) is more toxic to M. persicae than F. schultzei. This essential oil shows promise for developing pesticides to manage M. persicae.


2015 ◽  
Vol 17 (4 suppl 1) ◽  
pp. 769-773 ◽  
Author(s):  
R.L. CANSIAN ◽  
V. ASTOLFI ◽  
R.I. CARDOSO ◽  
N. PAROUL ◽  
S.S. ROMAN ◽  
...  

ABSTRACT The aim of this work was to evaluate the insecticidal and repellency activity of the essential oil of Cinnamomum camphora var. linaloolifera Y. Fujita (Ho-Sho) and Cinnamomumcamphora (L.) J Presl.var. hosyo (Hon-Sho), against the Sitophilus zeamais in maize grains. The essential oils were obtained by hydrodistillation and analyzed by GC-MS.The insecticidal activity was determined by the toxicity of different concentrations of essential oils during 24 hours of contact with the insects, in the absence of feed substrate. The Bioassays of repellency were conducted with lethal doses (LD50,LD25,and LD12.5) obtained from insecticidal bioassay. In order to compare the treatments the preference index (PI) was employed. The analysis of the essential oils of Cinnamomum camphora leaves indicated 68% of camphor and 9% of linalool for the variation Hon-Sho and 95% of linalool to the variation Ho-Sho. The variation Ho-Sho presented greatest insecticidal activity than the variation Hon-Sho against the Sitophiluszeamais, with LD50 of 0.35 μL/cm2, whereas in the variation Hon-Sho the ratewas 0.48 μL/cm2. However, considering only the concentrations of linalool and camphor of Ho-Sho and Hon-Sho, the lethal doses’ evaluation of these compounds were similar. The values of the preference index ranged from -0.3 to -0.8 for thevariation Ho-Sho and -0.2 to -0.7 for the variation Hon-Sho. The essential oils evaluated in this work showed repellent activity against Sitophiluszeamais in vitro and in trials performed in mini-silos.


2016 ◽  
Vol 8 (4) ◽  
pp. 2049-2054 ◽  
Author(s):  
K. K. Chahal ◽  
Ritima Bansal ◽  
Ramandeep Kaur

A laboratory experiment was conducted to study chemistry and insecticidal activity of bay leaf oil, its fractions and isolated compounds against stored grain pest of wheat i.e.Tribolium castaneum Herbst. Bay leaf essential oil extracted from dried and powdered bay leaves was subjected to column chromatography to have its fractions. Extensive column chromatography of polar fraction yielded Eugenol and 7, 7 Dimethyl-3-methylene bicyclo [2.2.1] heptan-4-ol which were identified by spectroscopic techniques. Bay leaf oil was tested for its insecticidal activity at five different concentrations in the range 4-12 mg g-1respectively against F1 generation of red rust flour beetle adults. Maximum inhibition was observed at 12 mg g-1concentration. The activity was both time and concentration dependent. The fractions of bay leaf essential oil and the compounds isolated were tested at 4mg g-1 concentration. Polar fraction was found to be more active as compared to nonpolar fraction as 100 and 53.1% mortality was obtained on 30th day for polar and nonpolar fractions, respectively. Comparison of eugenol and 7, 7 Dimethyl-3-methylene bicyclo [2.2.1] heptan-4-ol showed complete mortality on 33rd and 35thday respectively , which revealed that adults of T. castaneum were more susceptible to eugenol.The results indicated that bay leaf essential oil may have potential to control stored grain pest, T. castaneum.


2008 ◽  
Vol 20 (5) ◽  
pp. 383-385
Author(s):  
Sandra V. Clemente ◽  
Graciela Mareggiani ◽  
Berta E. Juárez ◽  
María E. Mendiondo ◽  
Catalina M. van Baren ◽  
...  

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