Characterization of the adenyl cyclase of rat kidney plasma membranes

1972 ◽  
Vol 266 (2) ◽  
pp. 524-542 ◽  
Author(s):  
Leonard R. Forte
Keyword(s):  
Plasma Membranes ◽  
Rat Kidney ◽  
Adenyl Cyclase

scholarly journals The characterization of rat kidney plasma membranes prepared by zonal centrifugation

1972 ◽  
Vol 129 (4) ◽  
pp. 919-928 ◽  
Author(s):  
R. G. Price ◽  
D. G. Taylor ◽  
D. Robinson
Keyword(s):  
Alkaline Phosphatase ◽  
Sucrose Gradient ◽  
Plasma Membranes ◽  
Rat Kidney ◽  
Marker Enzymes ◽  
Subcellular Organelles ◽  
High Degree ◽  
Isopycnic Centrifugation ◽  
Zonal Rotor

1. Plasma membranes were isolated from a 10000g-min pellet prepared from a renal cortical homogenate in 20mm-NaHCO3 by isopycnic centrifugation in a linear sucrose gradient in an ‘A’-type zonal rotor. 2. The preparation was characterized by electron microscopy, and alkaline phosphatase, 5′-nucleotidase, l-leucine β-naphthylamidase and l-leucine p-nitroanilidase activities were found to be selectively associated with the renal plasma membrane. 3. The preparation had a high degree of purity, as indicated by the presence of low activities of marker enzymes associated with subcellular organelles. A preliminary chemical analysis indicated that the chemical composition resembled that of plasma membranes of other tissues. 4. Plasma membranes were also prepared from tubular fragments and their enzyme contents were found to be similar to those of plasma membranes prepared from cortical homogenates. 5. l-Leucine β-naphthylamidase, l-leucine p-nitroanilidase and 5′-nucleotidase were not enriched to the same extent as alkaline phosphatase in the preparation of plasma membranes from tubular fragments. A possible explanation for this finding is discussed.

Characterization of Ascorbic Acid Uptake by Isolated Rat Kidney Cells

1991 ◽  
Vol 121 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Delores M. Bowers-Komro ◽  
Donald B. McCormick
Keyword(s):  
Ascorbic Acid ◽  
Rat Kidney ◽  
Acid Uptake ◽  
Kidney Cells ◽  
Isolated Rat Kidney ◽  
Isolated Rat

scholarly journals Characterization of cobalamin receptor sites in brush-border plasma membranes of the tapeworm Spirometra mansonoides.

1983 ◽  
Vol 258 (7) ◽  
pp. 4261-4265
Author(s):  
P A Friedman ◽  
P P Weinstein ◽  
J F Mueller ◽  
R H Allen
Keyword(s):  
Brush Border ◽  
Plasma Membranes ◽  
Receptor Sites

scholarly journals Characterization of calcium binding to adipocyte plasma membranes.

1976 ◽  
Vol 251 (17) ◽  
pp. 5345-5351
Author(s):  
J M McDonald ◽  
D E Bruns ◽  
L Jarett
Keyword(s):  
Calcium Binding ◽  
Plasma Membranes ◽  
Adipocyte Plasma Membranes
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