Ferric ion sequestering agents kinetics of iron release from ferritin to catechoylamides

Transient “kiss and run” interactions between endosomes containing iron-bound transferrin (Tf) and mitochondria have been shown to facilitate direct iron transfer in erythroid cells. In this study, we used superresolution three-dimensional (3D) direct stochastic optical reconstruction microscopy to show that Tf-containing endosomes directly interact with mitochondria in epithelial cells. We used live-cell time-lapse fluorescence microscopy, followed by 3D rendering, object tracking, and a distance transformation algorithm, to track Tf-endosomes and characterize the dynamics of their interactions with mitochondria. Quenching of iron sensor RDA-labeled mitochondria confirmed functional iron transfer by an interacting Tf-endosome. The motility of Tf-endosomes is significantly reduced upon interaction with mitochondria. To further assess the functional role of iron in the ability of Tf-endosomes to interact with mitochondria, we blocked endosomal iron release by using a Tf K206E/K534A mutant. Blocking intraendosomal iron release led to significantly increased motility of Tf-endosomes and increased duration of endosome–mitochondria interactions. Thus, intraendosomal iron regulates the kinetics of the interactions between Tf-containing endosomes and mitochondria in epithelial cells.

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Ferric ion sequestering agents. 11. Synthesis and kinetics of iron removal from transferrin of catechoyl derivatives of desferrioxamine B

Journal of Medicinal Chemistry ◽

10.1021/jm00357a022 ◽

1983 ◽

Vol 26 (3) ◽

pp. 439-442 ◽

Cited By ~ 28

Author(s):

Steven J. Rodgers ◽

Kenneth N. Raymond

Keyword(s):

Iron Removal ◽

Ferric Ion ◽

Desferrioxamine B ◽

Kinetics Of ◽

Derivatives Of

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Microbial Ferrous Ion Oxidation versus Ferric Ion Precipitation at Low Temperature Conditions

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.262.381 ◽

2017 ◽

Vol 262 ◽

pp. 381-384 ◽

Cited By ~ 2

Author(s):

Emmanuel Chinonso Chukwuchendo ◽

Tunde Victor Ojumu

Keyword(s):

Packed Column ◽

Ferrous Ion ◽

Design Management ◽

Ferric Ion ◽

Oxidation Rates ◽

Temperature Conditions ◽

First Order Kinetics ◽

Slow Kinetics ◽

Cold Condition ◽

Kinetics Of

Ferric ion precipitation in a typical bioleach operation is known to be inevitable. However, its management has been emphasized as critical to efficiently maximize the potential that bioleach technique offers, especially in heap bioleach systems. Although there are some studies on ferric ion precipitation, limited studies exist in the context of a cold condition which often prevails in some operations. This study investigated ferrous ion biooxidation and ferric ion precipitation under cold temperature conditions. The results showed that maximum ferrous ion oxidation rates of 1.05, 1.25, 1.51 and 1.60 mmol/L/h at 6, 7, 8 and 10°C respectively were achieved in packed column reactor. The results further showed a simultaneous ferric ion precipitation during the oxidation process. The kinetics of ferric ion precipitation follows a first order kinetics with the rate constants of 0.0066, 0.0074, 0.0080 and 0.011 h-1. Activation energies of 68 and 77 kJ/mol were obtained for both ferrous ion biooxidation and ferric ion precipitation respectively. Although slow kinetics are expected for both processes, the kinetics information may be useful for design management consideration at least to predict when bioleach liquor would likely run out of dissolved ferric ion.

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Mechanism and kinetics of iron release from ferritin by dihydroflavins and dihydroflavin analogs

Biochemistry ◽

10.1021/bi00612a021 ◽

1978 ◽

Vol 17 (19) ◽

pp. 4011-4017 ◽

Cited By ~ 142

Author(s):

Tim Jones ◽

Rob Spencer ◽

Christopher Walsh

Keyword(s):

Iron Release ◽

Mechanism And Kinetics ◽

Kinetics Of

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The effect of salt concentration on the iron-binding properties of human transferrin

Biochemical Journal ◽

10.1042/bj2010527 ◽

1982 ◽

Vol 201 (3) ◽

pp. 527-532 ◽

Cited By ~ 39

Author(s):

J Williams ◽

N D Chasteen ◽

K Moreton

Keyword(s):

Salt Concentration ◽

Binding Sites ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Iron Binding ◽

Iron Release ◽

Equilibrium Conditions ◽

Binding Properties ◽

Kinetics Of ◽

Terminal Site

The salt dependence of the iron-binding properties of transferrin was studied by urea/polyacrylamide-gel electrophoresis. The distribution of iron between the N-terminal and C-terminal binding sites under equilibrium conditions and the rates of release of iron from the two sites were studied. It was found that salt increases the thermodynamic stability of iron binding in the N-terminal site relative to the C-terminal site. Similar behaviour is observed for the kinetics of iron release, where salt retards the rate of removal of iron from the N-terminal site but facilitates removal from the C-terminal site.

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Kinetics of iron release from transferrin bound to the transferrin receptor at endosomal pH

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2011.06.003 ◽

2012 ◽

Vol 1820 (3) ◽

pp. 326-333 ◽

Cited By ~ 53

Author(s):

Ashley N. Steere ◽

Shaina L. Byrne ◽

N. Dennis Chasteen ◽

Anne B. Mason

Keyword(s):

Transferrin Receptor ◽

Iron Release ◽

Endosomal Ph ◽

Kinetics Of

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Antibacterial Activity and Iron Release of Organic-Inorganic Hybrid Biomaterials Synthesized via the Sol-Gel Route

Applied Sciences ◽

10.3390/app11199311 ◽

2021 ◽

Vol 11 (19) ◽

pp. 9311

Author(s):

Michelina Catauro ◽

Ylenia D’Errico ◽

Antonio D’Angelo ◽

Ronald J. Clarke ◽

Ignazio Blanco

Keyword(s):

Hybrid Materials ◽

Release Kinetics ◽

Sol Gel ◽

Antibacterial Properties ◽

Iron Release ◽

Biological Characterization ◽

Visible Spectroscopy ◽

E Coli ◽

Hybrid Biomaterials ◽

Kinetics Of

The aim of this work was the synthesis of hybrid materials of iron (II)-based therapeutic systems via the sol-gel method. Increasing amounts of polyethylene glycol (PEG 6, 12, 24, 50 wt%) were added to SiO2/Fe20 wt% to modulate the release kinetics of the drug from the systems. Fourier-transform infrared (FTIR) spectroscopy was used to study the interactions between different components in the hybrid materials. The release kinetics in a simulated body fluid (SBF) were investigated, and the amount of Fe2+ released was detected via ultraviolet-visible spectroscopy (UV-Vis) after reaction with ortho-phenanthroline. Furthermore, biological characterization was carried out. The bioactivity of the synthesized hybrid materials was evaluated via the formation of a layer of hydroxyapatite on the surface of samples soaked in SBF using spectroscopy. Finally, the potential antibacterial properties of seven different materials against two different bacteria—E. coli and S. aureus—were investigated.

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