[3H]Tryptamine: High affinity binding sites in rat brain

1982 ◽  
Vol 78 (4) ◽  
pp. 475-478 ◽  
Author(s):  
Kenneth J. Kellar ◽  
Caren S. Cascio
Keyword(s):  
Rat Brain ◽  
Binding Sites ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity

scholarly journals [3H]-ouabain binding sites in rat brain: distribution and properties assessed by quantitative autoradiography.

1992 ◽  
Vol 40 (6) ◽  
pp. 771-779 ◽  
Author(s):  
A A Maki ◽  
D G Baskin ◽  
W L Stahl
Keyword(s):  
Nervous System ◽  
Rat Brain ◽  
Cardiac Glycoside ◽  
Binding Sites ◽  
Quantitative Autoradiography ◽  
Affinity Binding ◽  
Ouabain Binding ◽  
High Affinity Binding ◽  
High Affinity ◽  
Kd Values

The anatomic distribution of high- and low-affinity cardiac glycoside binding sites in the nervous system is largely unknown. In the present study the regional distribution and properties of these sites were determined in rat brain by quantitative autoradiography (QAR). Two populations of cardiac glycoside binding sites were demonstrated with [3H]-ouabain, a specific inhibitor of Na,K-ATPases: (a) high-affinity binding sites with Kd values of 22-69 nM, which were blocked by erythrosin B, and (b) low-affinity binding sites with Kd values of 727-1482 nM. Sites with very low affinity for ouabain were not found by QAR. High- and low-affinity [3H]-ouabain binding sites were both found in all brain regions studied, including somatosensory cortex, thalamic and hypothalamic areas, medial forebrain bundle, amygdaloid nucleus, and caudate-putamen, although the distributions of high- and low-affinity sites were not congruent. Low-affinity [3H]-ouabain binding sites (Bmax = 222-358 fmol/mm2) were approximately twofold greater in number than high-affinity binding sites (Bmax = 76-138 fmol/mm2) in these regions of brain. Binding of [3H]-ouabain to both high- and low-affinity sites was blocked by Na+; however, low-affinity binding sites were less sensitive to inhibition by K+ (IC50 = 6.4 mM) than the high-affinity [3H]-ouabain binding sites (IC50 = 1.4 mM). The QAR method, utilizing [3H]-ouabain under standard conditions, is a valid method for studying modulation of Na,K-ATPase molecules in well-defined anatomic regions of the nervous system.

High-affinity binding sites for 125I-labelled pancreatic secretory phospholipase A2 in rat brain

1997 ◽  
Vol 49 (1-2) ◽  
pp. 120-126 ◽  
Author(s):  
Kumlesh K Dev ◽  
Christian Foged ◽  
Heidi Andersen ◽  
Tage Honoré ◽  
Jeremy M Henley
Keyword(s):  
Phospholipase A2 ◽  
Rat Brain ◽  
Binding Sites ◽  
Affinity Binding ◽  
Secretory Phospholipase A2 ◽  
High Affinity Binding ◽  
Secretory Phospholipase ◽  
High Affinity

Rat brain membranes possess two high-affinity binding sites for [3H]somatostatin

1985 ◽  
Vol 55 (2) ◽  
pp. 161-166 ◽  
Author(s):  
D.R. Weightman ◽  
C.A. Whitford ◽  
C.R. Snell ◽  
B.H. Hirst ◽  
D.E. Brundish ◽  
...  
Keyword(s):  
Rat Brain ◽  
Binding Sites ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity ◽  
Brain Membranes ◽  
Rat Brain Membranes

High affinity binding sites for a somatostatin-28 analog in rat brain

Life Sciences ◽  
1981 ◽  
Vol 28 (19) ◽  
pp. 2191-2198 ◽  
Author(s):  
Jean-Claude Reubi ◽  
Marilyn H. Perrin ◽  
Jean E. Rivier ◽  
Wylie Vale
Keyword(s):  
Rat Brain ◽  
Binding Sites ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity
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