Characterization by enriched polyclonal antibodies of developmentally regulated and cell type specific mouse testis antigens

Life Sciences ◽  
1992 ◽  
Vol 51 (6) ◽  
pp. 439-448 ◽  
Author(s):  
Lydia Lemaire ◽  
Annette Senftleben ◽  
Uwe A.O. Heinlein
Keyword(s):  
Polyclonal Antibodies ◽  
Mouse Testis ◽  
Cell Type ◽  
Developmentally Regulated ◽  
Cell Type Specific

Expression of the Ly-6 family proteins Lynx1 and Ly6H in the rat brain is compartmentalized, cell-type specific, and developmentally regulated

2013 ◽  
Vol 219 (6) ◽  
pp. 1923-1934 ◽  
Author(s):  
Morten Skøtt Thomsen ◽  
Betül Cinar ◽  
Majbrit Myrup Jensen ◽  
Ekaterina N. Lyukmanova ◽  
Mikhail A. Shulepko ◽  
...  
Keyword(s):  
Rat Brain ◽  
Cell Type ◽  
Developmentally Regulated ◽  
Cell Type Specific

scholarly journals Neuron-specific signatures in the chromosomal connectome associated with schizophrenia risk

Science ◽  
2018 ◽  
Vol 362 (6420) ◽  
pp. eaat4311 ◽  
Author(s):  
Prashanth Rajarajan ◽  
Tyler Borrman ◽  
Will Liao ◽  
Nadine Schrode ◽  
Erin Flaherty ◽  
...  
Keyword(s):  
Neural Progenitor Cells ◽  
Disease Risk ◽  
Three Dimensional ◽  
Cell Type ◽  
Contact Map ◽  
Developmentally Regulated ◽  
Risk Variants ◽  
Cell Type Specific ◽  
Spatial Genome Organization ◽  
Developmental Remodeling

To explore the developmental reorganization of the three-dimensional genome of the brain in the context of neuropsychiatric disease, we monitored chromosomal conformations in differentiating neural progenitor cells. Neuronal and glial differentiation was associated with widespread developmental remodeling of the chromosomal contact map and included interactions anchored in common variant sequences that confer heritable risk for schizophrenia. We describe cell type–specific chromosomal connectomes composed of schizophrenia risk variants and their distal targets, which altogether show enrichment for genes that regulate neuronal connectivity and chromatin remodeling, and evidence for coordinated transcriptional regulation and proteomic interaction of the participating genes. Developmentally regulated chromosomal conformation changes at schizophrenia-relevant sequences disproportionally occurred in neurons, highlighting the existence of cell type–specific disease risk vulnerabilities in spatial genome organization.

scholarly journals Changing Patterns of Gene Expression in Dictyostelium Prestalk Cell Subtypes Recognized by In Situ Hybridization with Genes from Microarray Analyses

2003 ◽  
Vol 2 (3) ◽  
pp. 627-637 ◽  
Author(s):  
Mineko Maeda ◽  
Haruyo Sakamoto ◽  
Negin Iranfar ◽  
Danny Fuller ◽  
Toshinari Maruo ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Cell Types ◽  
Cell Type ◽  
Developmentally Regulated ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
Changing Patterns ◽  
Cell Type Specific

ABSTRACT We used microarrays carrying most of the genes that are developmentally regulated in Dictyostelium to discover those that are preferentially expressed in prestalk cells. Prestalk cells are localized at the front of slugs and play crucial roles in morphogenesis and slug migration. Using whole-mount in situ hybridization, we were able to verify 104 prestalk genes. Three of these were found to be expressed only in cells at the very front of slugs, the PstA cell type. Another 10 genes were found to be expressed in the small number of cells that form a central core at the anterior, the PstAB cell type. The rest of the prestalk-specific genes are expressed in PstO cells, which are found immediately posterior to PstA cells but anterior to 80% of the slug that consists of prespore cells. Half of these are also expressed in PstA cells. At later stages of development, the patterns of expression of a considerable number of these prestalk genes changes significantly, allowing us to further subdivide them. Some are expressed at much higher levels during culmination, while others are repressed. These results demonstrate the extremely dynamic nature of cell-type-specific expression in Dictyostelium and further define the changing physiology of the cell types. One of the signals that affect gene expression in PstO cells is the hexaphenone DIF-1. We found that expression of about half of the PstO-specific genes were affected in a mutant that is unable to synthesize DIF-1, while the rest appeared to be DIF independent. These results indicate that differentiation of some aspects of PstO cells can occur in the absence of DIF-1.

The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina

2000 ◽  
Vol 78 (1-2) ◽  
pp. 26-37 ◽  
Author(s):  
D Baas ◽  
K.M Bumsted ◽  
J.A Martinez ◽  
F.M Vaccarino ◽  
K.C Wikler ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Mouse Retina ◽  
Cell Type ◽  
Developmentally Regulated ◽  
Cell Type Specific

Two MCAT elements of the SM α-actin promoter function differentially in SM vs. non-SM cells

1998 ◽  
Vol 275 (2) ◽  
pp. C608-C618 ◽  
Author(s):  
Ellen A. Swartz ◽  
A. Daniel Johnson ◽  
Gary K. Owens
Keyword(s):  
Transcriptional Activity ◽  
Polyclonal Antibodies ◽  
Cell Types ◽  
Actin Gene ◽  
Cell Type ◽  
Nuclear Extracts ◽  
Cell Type Specific ◽  
Actin Promoter ◽  
Specific Regulation ◽  
Related Proteins

Transcriptional activity of the smooth muscle (SM) α-actin gene is differentially regulated in SM vs. non-SM cells. Contained within the rat SM α-actin promoter are two MCAT motifs, binding sites for transcription enhancer factor 1 (TEF-1) transcriptional factors implicated in the regulation of many muscle-specific genes. Transfections of SM α-actin promoter-CAT constructs containing wild-type or mutagenized MCAT elements were performed to evaluate their functional significance. Mutation of the MCAT elements resulted in increased transcriptional activity in SM cells, whereas these mutations either had no effect or decreased activity in L6 myotubes or endothelial cells. High-resolution gel shift assays resolved several complexes of different mobilities that were formed between MCAT oligonucleotides and nuclear extracts from the different cell types, although no single band was unique to SM. Western blot analysis of nuclear extracts with polyclonal antibodies to conserved domains of the TEF-1 gene family revealed multiple reactive bands, some that were similar and others that differed between SM and non-SM. Supershift assays with a polyclonal antibody to the TEF-related protein family demonstrated that TEF-1 or TEF-1-related proteins were contained in the shifted complexes. Results suggest that the MCAT elements may contribute to cell type-specific regulation of the SM α-actin gene. However, it remains to be determined whether the differential transcriptional activity of MCAT elements in SM vs. non-SM is due to differences in expression of TEF-1 or TEF-1-related proteins or to unique (cell type specific) combinatorial interactions of the MCAT elements with other cis-elements and trans-factors.

Developmental and cell type-specific expression of αB-crystallin (αB-Cry) in mouse testis and epididymis

2010 ◽  
Vol 86 (2) ◽  
pp. 96-97
Author(s):  
M. Mollova ◽  
M. Stamenova ◽  
S. Zaprjanova ◽  
Y. Martinova ◽  
P. Rashev ◽  
...  
Keyword(s):  
Mouse Testis ◽  
Cell Type ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
Αb Crystallin ◽  
Cell Type Specific
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