α-selectivity and glycal formation are temperature dependent in glycosylation with sialic acid. Synthesis of a Neu5Acα(2-6)Gal thioglycoside building block

A sialic acid glycosyl phosphate building block was designed and synthesized. This building block was used to prepare α-sialylated oligosaccharides by automated solid-phase synthesis selectively.

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Reaction-Temperature-Dependent Supramolecular Isomerism of Coordination Networks Based on the Organometallic Building Block[CuI2(μ2-BQ)(μ2-OAc)2]

Angewandte Chemie International Edition ◽

10.1002/anie.200353463 ◽

2004 ◽

Vol 43 (19) ◽

pp. 2530-2534 ◽

Cited By ~ 172

Author(s):

Shigeyuki Masaoka ◽

Daisuke Tanaka ◽

Yasunori Nakanishi ◽

Susumu Kitagawa

Keyword(s):

Reaction Temperature ◽

Building Block ◽

Coordination Networks ◽

Temperature Dependent ◽

Supramolecular Isomerism

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ChemInform Abstract: Enantiomer Separation of (R,S)-2-(tert-Butyl)-3-methyl-4-imidazolidinone, a Chiral Building Block for Amino Acid Synthesis.

Chemischer Informationsdienst ◽

10.1002/chin.198631106 ◽

1986 ◽

Vol 17 (31) ◽

Author(s):

R. FITZI ◽

D. SEEBACH

Keyword(s):

Amino Acid ◽

Building Block ◽

Enantiomer Separation ◽

Acid Synthesis ◽

Amino Acid Synthesis ◽

Tert Butyl ◽

Chiral Building Block

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Engineering intracellular malonyl-CoA availability in microbial hosts and its impact on polyketide and fatty acid synthesis

Applied Microbiology and Biotechnology ◽

10.1007/s00253-020-10643-7 ◽

2020 ◽

Vol 104 (14) ◽

pp. 6057-6065 ◽

Cited By ~ 1

Author(s):

Lars Milke ◽

Jan Marienhagen

Keyword(s):

Fatty Acid ◽

Metabolic Engineering ◽

Fatty Acid Synthesis ◽

Building Block ◽

Acid Synthesis ◽

Microbial Synthesis ◽

Acetyl Coa ◽

Carboxylase Activity ◽

Cell Factories ◽

Malonyl Coa

AbstractMalonyl-CoA is an important central metabolite serving as the basic building block for the microbial synthesis of many pharmaceutically interesting polyketides, but also fatty acid–derived compounds including biofuels. Especially Saccharomyces cerevisiae, Escherichia coli, and Corynebacterium glutamicum have been engineered towards microbial synthesis of such compounds in recent years. However, developed strains and processes often suffer from insufficient productivity. Usually, tightly regulated intracellular malonyl-CoA availability is regarded as the decisive bottleneck limiting overall product formation. Therefore, metabolic engineering towards improved malonyl-CoA availability is essential to design efficient microbial cell factories for the production of polyketides and fatty acid derivatives. This review article summarizes metabolic engineering strategies to improve intracellular malonyl-CoA formation in industrially relevant microorganisms and its impact on productivity and product range, with a focus on polyketides and other malonyl-CoA-dependent products.Key Points• Malonyl-CoA is the central building block of polyketide synthesis.• Increasing acetyl-CoA supply is pivotal to improve malonyl-CoA availability.• Improved acetyl-CoA carboxylase activity increases availability of malonyl-CoA.• Fatty acid synthesis as an ambivalent target to improve malonyl-CoA supply.

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