Leukemogenic properties of AKR dualtropic (MCF) viruses: Amplification of murine leukemia virus-related antigens on thymocytes and acceleration of leukemia development in AKR mice

Virology ◽  
1981 ◽  
Vol 112 (2) ◽  
pp. 548-563 ◽  
Author(s):  
Paul V. O'Donnell ◽  
Elisabeth Stockert ◽  
Yuichi Obata ◽  
Lloyd J. Old
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Leukemia Development ◽  
Akr Mice ◽  
Murine Leukemia

scholarly journals Amplified env and gag products on AKR cells. Origin from different murine leukemia virus genomes.

1980 ◽  
Vol 151 (4) ◽  
pp. 975-979 ◽  
Author(s):  
J S Tung ◽  
E Fleissner
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Mouse Strains ◽  
Type Antigen ◽  
Mink Cell ◽  
Virus Expression ◽  
Virus Genomes ◽  
Akr Mice ◽  
Ecotropic Virus ◽  
Murine Leukemia

Thymocytes of AKR mice express two species of gp70, the envelope glycoprotein of murine leukemia virus (MuLV), encoded by the env gene. One is denoted Ec+ gp70 in reference to the type-antigen Ec and association with ecotropic virus. The other, Ec- gp70, resembles gp70 found also on thymocytes of mouse strains that are not overt producers of MuLV, and has no evident relation to ecotropic virus. Expression of Ec- gp70 type, but not of Ec+ gp70 type, is amplified with age on AKR thymocytes. In contrast, viral core polyproteins, encoded by the gag gene and simultaneously amplified with age, appear to be related to ecotropic virus. These observations imply selective amplification of products of env and gag genes from two sorts of provirus, a phenomenon which may be connected to the dual genetic origin of recombinant mink-cell-focus inducing viruses in AKR mice.

Genetic Mapping of a Murine Leukemia Virus-Inducing Locus of AKR Mice

Science ◽  
1972 ◽  
Vol 178 (4063) ◽  
pp. 860-862 ◽  
Author(s):  
W. P. Rowe ◽  
J. W. Hartley ◽  
T. Bremner
Keyword(s):  
Genetic Mapping ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Akr Mice ◽  
Murine Leukemia

scholarly journals Relationship of infectious murine leukemia virus and virus-related antigens in genetic crosses between AKR and the Fv-1 compatible strain C57L.

1976 ◽  
Vol 143 (1) ◽  
pp. 32-46 ◽  
Author(s):  
H Ikeda ◽  
W P Rowe ◽  
E A Boyse ◽  
E Stockert ◽  
H Sato ◽  
...  
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Specific Antigen ◽  
Viral Envelope ◽  
Progeny Testing ◽  
Chromosomal Genes ◽  
Relationship Of ◽  
Akr Mice ◽  
Murine Leukemia ◽  
High Output

In a further genetic study of murine leukemia virus (MuLV) and its components we examined the backcross C57L X (C57L X AKR). This population was selected because strains AKR and C57L are both Fv-1n, and the restriction which the Fu-1b allele imposes on the output of virus was thereby obviated. The segregants were scored for three characters: (a) infectious Gross-AKR-type MuLV (V), in the tail; (b) group-specific antigen indicative of p30 internal viral protein, in spleen; and (c) GIX antigen, now thought to be indicative of gp69/71 viral envelope glycoprotein, on thymocytes. Our conclusions are: (a) It is confirmed that the AKR mouse has two unlinked chromosomal genes, Akv-1 and Akv-2, each of which can independently give rise to the life-long high output of MuLV that is characteristic of AKR mice. (b) Of the eight phenotypes that could possibly be derived from segregation of the three pairs of independent alternative traits, seven were observed, but on progeny testing only three were shown to reflect stably heritable genotypes; these were V+p30+GIX+ and V-p30-GIX- (the parental types) and V-p30+GIX+. A third, newly identified AKR gene, designated Akvp, segregating independently of Akv-1 and Akv-2, also determines expression of p30 and GIX but in this case independently of XC-detectable MuLV. (c) The four remaining observed phenotypes, which did not breed true on progeny testing, involved mostly antigen-negative parents yielding antigen-positive progeny; it is likely that these discrepancies represented suppression of phenotype by a maternal resistance factor.

scholarly journals QUANTITATIVE STUDIES OF NATURALLY OCCURRING MURINE LEUKEMIA VIRUS INFECTION OF AKR MICE

1972 ◽  
Vol 135 (2) ◽  
pp. 429-436 ◽  
Author(s):  
Wallace P. Rowe ◽  
Theodore Pincus
Keyword(s):  
Virus Infection ◽  
Murine Leukemia Virus ◽  
Infectious Virus ◽  
Leukemia Virus ◽  
Organ Distribution ◽  
Thymic Lymphoma ◽  
Quantitative Studies ◽  
Naturally Occurring ◽  
Akr Mice ◽  
Murine Leukemia

Quantitative studies were made of the organ distribution of murine leukemia virus in AKR mice of various ages. Infectious virus first appeared shortly before or after birth and was continuously present in all mice thereafter. Highest infectivity titers were found in uterus and bone, with spleen slightly lower. Virus titers in normal thymus were relatively low, but increased significantly with the development of thymic lymphoma. The level of viremia decreased after the 1st month of life, but increased sharply in lymphomatous mice.

scholarly journals Expression of mink cell focus-forming murine leukemia virus-related transcripts in AKR mice.

1987 ◽  
Vol 61 (3) ◽  
pp. 876-882 ◽  
Author(s):  
A S Khan ◽  
F Laigret ◽  
C P Rodi
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Mink Cell ◽  
Akr Mice ◽  
Murine Leukemia

scholarly journals AUTOGENOUS IMMUNITY TO ENDOGENOUS RNA TUMOR VIRUS

1973 ◽  
Vol 138 (1) ◽  
pp. 194-208 ◽  
Author(s):  
J. N. Ihle ◽  
M. Yurconic ◽  
M. G. Hanna
Keyword(s):  
Murine Leukemia Virus ◽  
Gradient Analysis ◽  
Leukemia Virus ◽  
Age Groups ◽  
Rat Serum ◽  
High Incidence ◽  
Tumor Virus ◽  
Akr Mice ◽  
Murine Leukemia ◽  
Precipitation Test

The radioimmune precipitation assay using 3H-labeled AKR leukemia virus was applied to the detection and quantitation of natural serum antibodies directed against endogenous murine leukemia virus (MuLV) envelope antigens B6C3F1 and BALB/c mice, which have low natural incidences of leukemia and lymphoma, and AKR mice, which have a high incidence, were used in this study. Sera from mice of various age groups were assayed. A marked difference in age-associated levels of the autogenous immune response to endogenous murine leukemia virus was detected, and the quantitative differences among these strains were inversely related to the incidence of lymphoma. The radioimmune precipitation test as applied was 500 times more sensitive than virus neutralization. That the reactions we have observed are specific is suggested by several lines of evidence, including the nonreactivity of normal hamster and absorbed rat serum, the positive reaction of absorbed rat anti-AKR serum, the inhibition of precipitation of labeled virus by purified unlabeled virus, and isopycnic gradient analysis of the reactive products.

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