Age-associated changes in the B-cell repertoire: effect of age on RAG-1 gene expression in murine bone marrow

1994 ◽  
Vol 40 (3) ◽  
pp. 287-289 ◽  
Author(s):  
Arie Ben-Yehuda ◽  
Paul Szabo ◽  
Marc E. Weksler
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
B Cell ◽  
Cell Repertoire ◽  
Murine Bone Marrow ◽  
B Cell Repertoire ◽  
Rag 1 ◽  
Effect Of Age

scholarly journals Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells.

1993 ◽  
Vol 177 (4) ◽  
pp. 915-923 ◽  
Author(s):  
E A Faust ◽  
D C Saffran ◽  
D Toksoz ◽  
D A Williams ◽  
O N Witte
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
B Cells ◽  
Expression Patterns ◽  
Porous Membrane ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Gene Expression Patterns ◽  
Murine Bone Marrow ◽  
Interleukin 7 ◽  
Rag 1

Long-term bone marrow cultures have been useful in determining gene expression patterns in pre-B cells and in the identification of cytokines such as interleukin 7 (IL-7). We have developed a culture system to selectively grow populations of B lineage restricted progenitors (pro-B cells) from murine bone marrow. Pro-B cells do not grow in response to IL-7, Steel locus factor (SLF), or a combination of the two. c-kit, the SLF receptor, and the IL-7 receptor are both expressed by pro-B cells, indicating that the lack of response is not simply due to the absence of receptors. Furthermore, SLF is not necessary for the growth of pro-B cells since they could be expanded on a stromal line derived from Steel mice that produces no SLF. IL-7 responsiveness in pre-B cells is associated with an increase in n-myc expression and is correlated with immunoglobulin (Ig) gene rearrangements. Although members of the ets family of transcription factors and the Pim-1 kinase are expressed by pro-B cells, n-myc is not expressed. Pro-B cells maintain Ig genes in the germline configuration, which is correlated with a low level of recombination activating genes 1 and 2 (Rag-1 and 2) mRNA expression, but high expression of sterile mu and terminal deoxynucleotidyl transferase. Pro-B cells are unable to grow separated from the stromal layer by a porous membrane, indicating that stromal contact is required for growth. These results suggest that pro-B cells are dependent on alternative growth signals derived from bone marrow stroma and can be distinguished from pre-B cells by specific patterns of gene expression.

Effect of age on the expressed B cell repertoire: role of B cell subsets

1993 ◽  
Vol 5 (9) ◽  
pp. 1035-1039 ◽  
Author(s):  
A. Hu ◽  
D. Ehleiter ◽  
A. Ben-Yehuda ◽  
R. Schwab ◽  
C. Russo ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
B Cell Subsets ◽  
Effect Of Age

scholarly journals Type I IFN sets the stringency of B cell repertoire selection in the bone marrow

1999 ◽  
Vol 11 (2) ◽  
pp. 279-288 ◽  
Author(s):  
Rita Vasconcellos ◽  
Deborah Braun ◽  
Antonio Coutinho ◽  
Jocelyne Demengeot
Keyword(s):  
Bone Marrow ◽  
B Cell ◽  
Type I ◽  
Type I Ifn ◽  
Cell Repertoire ◽  
Repertoire Selection ◽  
B Cell Repertoire

scholarly journals Comparison of the fetal and adult functional B cell repertoires by analysis of VH gene family expression.

1988 ◽  
Vol 168 (2) ◽  
pp. 589-603 ◽  
Author(s):  
H D Jeong ◽  
J M Teale
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
B Cells ◽  
Gene Family ◽  
B Cell ◽  
Cell Lineage ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Vh Gene

The functional B cell repertoire in BALB/c mice was assessed at various stages in ontogeny. This was done by analyzing VH gene family expression using the sensitive technique of in situ hybridization. The B cell repertoire was probed with the mitogen, LPS, and the antigen DNP. DNP was chosen because B cells responsive to this hapten appear very early in ontogeny. The APCs that developed after stimulation with LPS or DNP were analyzed for VH gene expression by in situ hybridization of individual cells using radiolabeled VH gene family probes. The results indicated that VH gene expression in fetal B cells after stimulation was distinct from adult B cells in that there was a biased expression of D proximal families. The results indicated that this bias was associated with developmental age and not a given differentiation stage in the B cell lineage. In addition, stimulation of fetal B cells with DNP resulted in a large increase in expression of member(s) of VH 36-60, suggesting that the early appearance of DNP-responsive B cells is not strictly correlated with preferential rearrangement of D proximal families, VH 7183 and VH Q52. However, the results suggested that a large proportion of pre-B cells that preferentially rearrange D proximal families early in ontogeny become part of the functional developing repertoire.

scholarly journals Impaired regeneration of the peripheral B cell repertoire from bone marrow following lymphopenia in old mice

2001 ◽  
Vol 31 (2) ◽  
pp. 500-505 ◽  
Author(s):  
Fang Li ◽  
Fang Jin ◽  
Antonio Freitas ◽  
Paul Szabo ◽  
Marc E. Weksler
Keyword(s):  
Bone Marrow ◽  
B Cell ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Old Mice

scholarly journals Role of variable region gene expression and environmental selection in determining the antiphosphorylcholine B cell repertoire.

1983 ◽  
Vol 158 (6) ◽  
pp. 1948-1961 ◽  
Author(s):  
N R Klinman ◽  
M R Stone
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
B Cell ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Variable Region ◽  
Precursor Cells ◽  
Cell Repertoire ◽  
Region Gene ◽  
B Cell Repertoire

To evaluate the role of environmental selective processes, as opposed to variable region gene expression, in the determination of B cell repertoire expression, we have assessed the phosphorylcholine (PC)-specific repertoire of precursor cells that remain in bone marrow cell populations after the removal of surface immunoglobulin (sIg)-bearing cells. Such cells are assumed to represent a stage in B cell maturation before the expression of sIg, and thus at a time when they have not as yet interfaced with environmental influences that operate through sIg receptors such as antigenic stimulation, tolerance, or antiidiotypic regulation. The repertoire as expressed in these cells, therefore, should reflect the readout of immunoglobulin variable region genes as they are expressed in progenitors to B cells. The results of these studies indicate that, as in mature primary B cell pools of BALB/c mice, the majority of PC-responsive sIg- bone marrow cells are of the T15 clonotype. Thus, environmental selective mechanisms would not appear to be required for the high frequency of B cells of the T15 idiotype in the primary B cell repertoire of BALB/c mice. Analysis of the sIg- bone marrow cells in (CBA/N X BALB/c)F1 male mice demonstrated that the deficit of PC-responsive mature B cells, which is a characteristic of this murine strain, must occur after receptor expression, since a normal frequency of PC-responsive and T15-expressing cells is present in their sIg- bone marrow population. Finally, these same mice were used to obtain bone marrow cell preparations from individual leg bones, so as to permit an analysis of the occurrence of T15+ and T15- clonotypes within individual bone marrow populations. The findings from these studies indicate that T15+ B cells occur as a high frequency event within bone marrow generative cell pools. Furthermore, bone marrow populations that are positive for PC-responsive precursor cells often display multiple copies of such precursor cells that are exclusively either T15+ or T15-. This finding indicates that clonal expansion of cells within the B cell lineage apparently occurs before immunoglobulin receptor acquisition.

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