159 Pharmacologically active amines as in vitro inhibitors of Junin virus

1994 ◽  
Vol 23 ◽  
pp. 121
Keyword(s):  
Junin Virus ◽  
Pharmacologically Active ◽  
Junín Virus

Polysulfonates Derived from Metal Thiolate Complexes as Inhibitors of HIV-1 and Various other Enveloped Viruses In Vitro

2002 ◽  
Vol 13 (3) ◽  
pp. 185-195 ◽  
Author(s):  
Donald E Bergstrom ◽  
Xiaoping Lin ◽  
Troy D Wood ◽  
Myriam Witvrouw ◽  
Satoru Ikeda ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Palladium Complex ◽  
Platinum Complex ◽  
Cadmium Complex ◽  
Junin Virus ◽  
Tacaribe Virus ◽  
Junín Virus ◽  
Hiv 1 ◽  
Hsv 1

Sodium 2-mercaptoethanesulfonate reacts with the metal ions Pd(II), Pt(II), Ag(I), Cd(II) and Zn(II) to yield complexes containing multiple anionic sulfonate sites. On the basis of spectroscopic and other analytical data the complexes were assigned the tentative molecular formulas: Pd6(SCH2CH2SO3Na)12, Ptn(SCH2CH2SO3Na)2n+2, Agn(SCH2CH2SO3Na)n, Na2Zn4(SCH2CH2SO3Na)10, and Na2Cd4(SCH2CH2SO3Na)10. The complexes displayed a variety of differences in activity towards DNA and RNA viruses. The platinum complex showed no measurable cytotoxicity and exhibited a spectrum of antiviral activity resembling that of dextran sulfate. It was active against HIV-1 and HIV-2, herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), thymidine kinase-deficient HSV-1, human cytomegalovirus, vesicular stomatitis virus (VSV), influenza A virus, respiratory syncytial virus (RSV), Sindbis virus, Junin virus and Tacaribe virus. The palladium complex also showed no measurable cytotoxicity, but was completely inactive against most viruses, with one notable exception: both HIV-1 and HIV-2 were substantially inhibited by the palladium complex. The silver complex showed significantly less antiviral activity and greater cytotoxicity than the platinum complex but did show some selectivity against RSV. The zinc complex showed only modest activity against VSV, RSV, Junin virus, and Tacaribe virus, and like the silver compound was more cytotoxic than either the platinum or palladium complex. The cadmium complex was toxic to all of the cell lines used for in vitro evaluation of antiviral activity. Based on these results, the platinum and palladium compounds appear to be promising candidates for further studies, that is, as vaginal microbicides in the prevention of genital HIV and/or HSV transmission.

In vitro selection of Junin virus antigenic variants

1993 ◽  
Vol 128 (3-4) ◽  
pp. 389-394 ◽  
Author(s):  
Laura E. Alché ◽  
Celia E. Coto
Keyword(s):  
In Vitro Selection ◽  
Junin Virus ◽  
Junín Virus ◽  
Selection Of

scholarly journals In vitro infection of murine macrophages with Junin virus.

1982 ◽  
Vol 35 (1) ◽  
pp. 356-358 ◽  
Author(s):  
P H González ◽  
J S Lampuri ◽  
C E Coto ◽  
R P Laguens
Keyword(s):  
Junin Virus ◽  
Murine Macrophages ◽  
Junín Virus

Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro

2008 ◽  
Vol 135 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Eliana G. Acosta ◽  
Andrea C. Bruttomesso ◽  
Juan A. Bisceglia ◽  
Mónica B. Wachsman ◽  
Lydia R. Galagovsky ◽  
...  
Keyword(s):  
Virus Replication ◽  
Junin Virus ◽  
Synthetic Derivatives ◽  
Junín Virus

scholarly journals Machupo Virus Expressing GPC of the Candid#1 Vaccine Strain of Junin Virus Is Highly Attenuated and Immunogenic

2015 ◽  
Vol 90 (3) ◽  
pp. 1290-1297 ◽  
Author(s):  
Takaaki Koma ◽  
Michael Patterson ◽  
Cheng Huang ◽  
Alexey V. Seregin ◽  
Payal D. Maharaj ◽  
...  
Keyword(s):  
Mouse Model ◽  
Vaccine Strain ◽  
Causative Agent ◽  
Transmembrane Domain ◽  
Hemorrhagic Fever ◽  
Single Amino Acid ◽  
Junin Virus ◽  
Machupo Virus ◽  
Junín Virus

ABSTRACTMachupo virus (MACV) is the causative agent of Bolivian hemorrhagic fever. Our previous study demonstrated that a MACV strain with a single amino acid substitution (F438I) in the transmembrane domain of glycoprotein is attenuated but genetically unstable in mice. MACV is closely related to Junin virus (JUNV), the causative agent of Argentine hemorrhagic fever. Others and our group have identified the glycoprotein to be the major viral factor determining JUNV attenuation. In this study, we tested the compatibility of the glycoprotein of the Candid#1 live-attenuated vaccine strain of JUNV in MACV replication and its ability to attenuate MACVin vivo. Recombinant MACV with the Candid#1 glycoprotein (rMACV/Cd#1-GPC) exhibited growth properties similar to those of Candid#1 and was genetically stablein vitro. In a mouse model of lethal infection, rMACV/Cd#1-GPC was fully attenuated, more immunogenic than Candid#1, and fully protective against MACV infection. Therefore, the MACV strain expressing the glycoprotein of Candid#1 is safe, genetically stable, and highly protective against MACV infection in a mouse model.IMPORTANCECurrently, there are no FDA-approved vaccines and/or treatments for Bolivian hemorrhagic fever, which is a fatal human disease caused by MACV. The development of antiviral strategies to combat viral hemorrhagic fevers, including Bolivian hemorrhagic fever, is one of the top priorities of the Implementation Plan of the U.S. Department of Health and Human Services Public Health Emergency Medical Countermeasures Enterprise. Here, we demonstrate for the first time that MACV expressing glycoprotein of Candid#1 is a safe, genetically stable, highly immunogenic, and protective vaccine candidate against Bolivian hemorrhagic fever.

In vitro anti-junin virus activity of a peptide isolated from Melia azedarach L. leaves

1998 ◽  
Vol 10 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Viviana Castilla ◽  
Andrea A Barquero ◽  
Susana E Mersich ◽  
Celia E Coto
Keyword(s):  
Melia Azedarach ◽  
Junin Virus ◽  
Virus Activity ◽  
Junín Virus

scholarly journals Zinc-binding properties of Junín virus nucleocapsid protein

2001 ◽  
Vol 82 (1) ◽  
pp. 121-128 ◽  
Author(s):  
M. Alejandra Tortorici ◽  
P. Daniel Ghiringhelli ◽  
Mario E. Lozano ◽  
César G. Albariño ◽  
Víctor Romanowski
Keyword(s):  
Nucleocapsid Protein ◽  
Zinc Binding ◽  
Dual Function ◽  
Genome Replication ◽  
Divalent Metal Ions ◽  
Junin Virus ◽  
N Protein ◽  
Carboxy Terminal ◽  
Junín Virus

The arenavirus nucleocapsid protein (N) is a highly basic 63 kDa protein with a dual function during the virus life-cycle. First, it is involved in essential steps of genome replication, promoting the synthesis of the full-length antigenomic copy of S RNA, and second it associates with the genomic RNA to form the nucleocapsid. We have expressed the N protein of Junín virus in E. coli and shown that it binds zinc in vitro. This property is in agreement with the presence in the carboxy-terminal region of the N protein of the CX2HX23CX4C sequence, which resembles a classical zinc-finger motif. The specificity for zinc binding was demonstrated by competition with other divalent metal ions. The ability of the predicted motif to bind zinc was established by analysis of a series of N mutants, including truncated variants and amino acid substitutions. In addition, alternative zinc-binding sites were found.

scholarly journals Junín Virus Infection of Human Hematopoietic Progenitors Impairs In Vitro Proplatelet Formation and Platelet Release via a Bystander Effect Involving Type I IFN Signaling

2010 ◽  
Vol 6 (4) ◽  
pp. e1000847 ◽  
Author(s):  
Roberto G. Pozner ◽  
Agustín E. Ure ◽  
Carolina Jaquenod de Giusti ◽  
Lina P. D'Atri ◽  
Joseph E. Italiano ◽  
...  
Keyword(s):  
Virus Infection ◽  
Bystander Effect ◽  
Type I ◽  
Type I Ifn ◽  
Hematopoietic Progenitors ◽  
Junin Virus ◽  
Platelet Release ◽  
Proplatelet Formation ◽  
Junín Virus

Inhibitory Effect of Thiosemicarbazone Derivatives on Junin Virus Replication In Vitro

2003 ◽  
Vol 14 (2) ◽  
pp. 99-105 ◽  
Author(s):  
Cybele C García ◽  
Beatriz N Brousse ◽  
Marla J Carlucci ◽  
Albertina G Moglioni ◽  
Miriam Martins Alho ◽  
...  
Keyword(s):  
Virus Replication ◽  
Inhibitory Effect ◽  
Haemorrhagic Fever ◽  
Inhibition Assay ◽  
Aromatic Ketones ◽  
Junin Virus ◽  
Structure Activity ◽  
Junín Virus ◽  
Replicative Cycle

The inhibitory effect of several thiosemicarbazones (TSCs), synthesized from aromatic ketones and terpenones, and their heterocyclic thiadiazoline (TDZ) derivatives, was investigated against Junin virus (JUNV), an arenavirus agent of Argentine haemorrhagic fever. From the 25 compounds tested, six compounds belonging to the TSC group were found to be selective inhibitors of JUNV, with EC50 values determined by a virus yield inhibition assay in the range 3.4–12.5 μM, and selectivity indices greater than 10. By contrast, most of the TDZs obtained by heterocyclization of the TSCs were not active against JUNV. No conclusive structure-activity relationships could be established but systematically higher activity was associated to TSCs derived from aromatic ketones. The mode of action of one of the most active compound, the 3,4-dihydronaphtalen-1(2 H)one thiosemicarbazone (tetralone thiosemicarbazone), was studied further. This TSC lacked virucidal effects on JUNV virions. Results from time of addition experiments and viral protein expression assays suggest that tetralone thiosemicarbazone inhibited a late stage in the replicative cycle of JUNV.

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