A novel cultivation technique for long-term maintenance of bloodstream form trypanosomes in vitro

1995 ◽  
Vol 70 (1-2) ◽  
pp. 157-166 ◽  
Author(s):  
Friedemann Hesse ◽  
Paul M. Selzer ◽  
Kerstin Mühlstädt ◽  
Michael Duszenko
Keyword(s):  
Bloodstream Form ◽  
Cultivation Technique ◽  
Term Maintenance

Long-term maintenance of reaggregated hypothalamic cultures developed from embryonic rat hypothalamus: prostaglandin release during synaptogenesis in vitro

1978 ◽  
Vol 34 (1) ◽  
pp. 159-171
Author(s):  
A. Gyevai ◽  
P.J. Chapple ◽  
W.H. Douglas
Keyword(s):  
Adult Animal ◽  
Morphological Differentiation ◽  
Adult Rat ◽  
Monolayer Cultures ◽  
Prostaglandin Release ◽  
Pge2 Concentration ◽  
The Media ◽  
Term Maintenance

Hypothalamic aggregate cultures were developed from hypothalami taken from rat embryos at 17–19 days gestation. The aggregate cultures exhibited a prominent morphological differentiation during 3–4 weeks in culture. The fine structure of the synapses formed in the aggregates resembled synapses in tha adult animal. During synaptogenesis the aggregates spontaneously release prostaglandin E2 (PGE2). The amount of PGE2 released in the media was reversed upon the morphological differentiation of the hypothalamic cultures. Media containing a higher PGE2 concentration increased the extracellular prolactin accumulation in monolayer cultures developed from adult rat hypophysis.

scholarly journals Gene transfer into hematopoietic stem cells: long-term maintenance of in vitro activated progenitors without marrow ablation.

1994 ◽  
Vol 91 (1) ◽  
pp. 350-354 ◽  
Author(s):  
D. Bienzle ◽  
A. C. Abrams-Ogg ◽  
S. A. Kruth ◽  
J. Ackland-Snow ◽  
R. F. Carter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
Marrow Ablation ◽  
Term Maintenance

Pancreatic small cells: Analysis of quiescence, long-term maintenance and insulin expression in vitro

2007 ◽  
Vol 313 (5) ◽  
pp. 931-942 ◽  
Author(s):  
M. Petropavlovskaia ◽  
C.A. Bodnar ◽  
L.A. Behie ◽  
L. Rosenberg
Keyword(s):  
Small Cells ◽  
Insulin Expression ◽  
Term Maintenance

scholarly journals Oligodendrocytes support axonal transport and maintenance via exosome secretion

2019 ◽  
Author(s):  
Carsten Frühbeis ◽  
Wen Ping Kuo-Elsner ◽  
Christina Müller ◽  
Kerstin Barth ◽  
Leticia Peris ◽  
...  
Keyword(s):  
Axonal Transport ◽  
Glial Cells ◽  
Axonal Degeneration ◽  
Specific Gene ◽  
Neuronal Viability ◽  
Mouse Mutants ◽  
Gene Defects ◽  
Term Maintenance

AbstractNeurons extend long axons that require maintenance and are susceptible to degeneration. Long-term integrity of axons depends on intrinsic mechanisms including axonal transport and extrinsic support from adjacent glial cells. The mechanisms of support provided by myelinating oligodendrocytes to underlying axons are only partly understood. Oligodendrocytes release extracellular vesicles (EVs) with properties of exosomes, which upon delivery to neurons improve neuronal viability in vitro. Here, we show that oligodendroglial exosome secretion is impaired in two mouse mutants exhibiting secondary axonal degeneration due to oligodendrocyte-specific gene defects. Wildtype oligodendroglial exosomes support neurons by improving the metabolic state and promoting axonal transport in nutrient deprived neurons. Mutant oligodendrocytes release less exosomes that share a common signature of underrepresented proteins. Notably, mutant exosomes lack the ability to support nutrient deprived neurons and to promote axonal transport. Together, these findings indicate that glia to neuron exosome transfer promotes neuronal long-term maintenance by facilitating axonal transport, providing a novel mechanistic link between myelin diseases and secondary loss of axonal integrity.

Long-term (35 years) cryopreservation of Echinococcus multilocularis metacestodes

Parasitology ◽  
2020 ◽  
Vol 147 (9) ◽  
pp. 1048-1054
Author(s):  
Teivi Laurimäe ◽  
Philipp A. Kronenberg ◽  
Cristian A. Alvarez Rojas ◽  
Theodor W. Ramp ◽  
Johannes Eckert ◽  
...  
Keyword(s):  
Echinococcus Multilocularis ◽  
Alveolar Echinococcosis ◽  
Geographic Origin ◽  
Experimental Animals ◽  
Long Term Storage ◽  
Term Maintenance ◽  
Term Storage

AbstractThe metacestode of Echinococcus multilocularis is the etiological agent of alveolar echinococcosis. The metacestode stage used for research is maintained in rodents by serial passages. In order to determine whether cryopreservation of E. multilocularis metacestodes would be suitable for long-term maintenance and replace serial passages, isolates of different geographic origin were cryopreserved in 1984–1986. The aim of the current study was to test the viability of cryopreserved isolates following long-term cryopreservation (up to 35 years) and to determine the phylogenetic clades these isolates belonged to. Cryopreserved isolates were tested for viability in vitro and in vivo in gerbils. In vitro results of 5 isolates indicated protoscolex survival in 13 of 17 experiments (76%) and metacestode survival in 5 of 12 (42%) in vivo experiments. In vivo results showed ‘abortive lesions’ in 13 of the 36 animals, 15 were negative and 8 harboured proliferating metacestode tissue containing protoscoleces. Genetic analysis confirmed the isolates belonged to European, Asian and North-American clades. In conclusion, the results of the current study indicate that metacestodes of E. multilocularis are able to survive long-term cryopreservation. Therefore, cryopreservation is a suitable method for long-term storage of E. multilocularis metacestode isolates and reduces the number of experimental animals.

scholarly journals In vitro culture on Matrigel favors the long-term maintenance of rat zona glomerulosa-cell differentiated phenotype

2006 ◽  
Author(s):  
Raffaella Spinazzi ◽  
Lucia Petrelli ◽  
Diego Guidolin ◽  
Gianni Carraro ◽  
Valentina Casale ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Zona Glomerulosa ◽  
Term Maintenance
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