The prognostic value of immunohistochemical estrogen receptor analysis in paraffin-embedded and frozen sections versus that of steroid-binding assays

By the conventional steroid-binding assay method for receptor, 3% of 1,095 primary breast cancers (or 10.6% of 263 premenopausal tumors) were classified as negative for estrogen receptor (ER), but positive for progesterone receptor (PR). The true ER status in this rare group of tumors was further investigated by the enzyme-immunoassay (EIA) or immunocytochemical (ICA) staining method using monoclonal antibodies H222 and D547. Immunoreactive ER was present in nine ER-/PR+ tumors studied, whereas it was not detectable in nine age-matched ER-/PR- tumors. Immunoreactive ER was also present in 24 ER+ breast cancers studied, and was particularly higher in tumors that were PR+. Measurement of immunoreactive ER by monoclonal antibody method provides certain advantages over the conventional dextran-coated charcoal (DCC) method, especially in ER-/PR+ tumors.

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Estrogenicity of styrene oligomers and assessment of estrogen receptor binding assays.

Environmental Health Perspectives ◽

10.1289/ehp.110-a384 ◽

2002 ◽

Vol 110 (7) ◽

Cited By ~ 2

Author(s):

Katsutoshi Ohno ◽

Yukimasa Azuma ◽

Katsuhiro Date ◽

Shigeru Nakano ◽

Toru Kobayashi ◽

...

Keyword(s):

Estrogen Receptor ◽

Receptor Binding ◽

Binding Assays ◽

Estrogen Receptor Binding

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Steroid specificity of the putative DHB receptor: evidence that the receptor is not 11βHSD

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1998.275.1.e124 ◽

1998 ◽

Vol 275 (1) ◽

pp. E124-E131 ◽

Cited By ~ 3

Author(s):

Karen E. Sheppard ◽

Karen Khoo ◽

Zygmunt S. Krozowski ◽

Kevin X. Z. Li

Keyword(s):

Steroid Receptors ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Rat Colon ◽

Colonic Crypt ◽

Binding Assays ◽

Ovary Cells ◽

High Affinity ◽

Steroid Binding ◽

Crypt Cells

Recently, we identified a novel putative nuclear receptor in colonic crypt cells distinct from both mineralocorticoid receptor and glucocorticoid receptor, with high affinity for 11-dehydrocorticosterone (11-DHB) (33). In the present study, competitive nuclear binding assays demonstrated that this site has a unique steroid binding specificity that distinguishes it from other steroid receptors. Western blot analysis showed the presence of 11β-hydroxysteroid dehydrogenase-2 (11βHSD2) but not 11βHSD1 in colonic crypt cells and showed that 11βHSD2 was present in the nuclear pellet. Differences in steroid specificity between the putative DHB receptor and inhibition of 11βHSD activity indicate that binding is not to the enzyme. Furthermore, modified Chinese hamster ovary cells transfected with the 11βHSD2 gene express nuclear 11βHSD2 but not a nuclear DHB binding site. In conclusion, these data support the existence of a novel nuclear DHB receptor in rat colon that is distinct from the classic steroid receptors and from both 11βHSD1 and 11βHSD2.

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Steroid Hormone Receptor Expression In Malignant Pleural Mesothelioma: Estrogen Receptor Beta Isoforms Have Prognostic Value

10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a5337 ◽

2012 ◽

Author(s):

Cormac J. Jennings ◽

Anthony O'Grady ◽

Robert Cummins ◽

Bruno Murer ◽

Elaine W. Kay ◽

...

Keyword(s):

Estrogen Receptor ◽

Hormone Receptor ◽

Prognostic Value ◽

Steroid Hormone ◽

Estrogen Receptor Beta ◽

Receptor Expression ◽

Steroid Hormone Receptor ◽

Pleural Mesothelioma ◽

Hormone Receptor Expression ◽

Receptor Beta

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Prognostic Value of Histochemical Evaluation of Steroid Binding Sites in Breast Tumors

The International Journal of Biological Markers ◽

10.1177/172460088800300309 ◽

1988 ◽

Vol 3 (3) ◽

pp. 197-202 ◽

Cited By ~ 1

Author(s):

P. Lampertico ◽

F. Stagni ◽

P. Crosignani

Keyword(s):

Binding Sites ◽

Prognostic Value ◽

Independent Predictor ◽

Breast Tumors ◽

Breast Cancer Patients ◽

Disease Free Interval ◽

Free Interval ◽

Steroid Binding ◽

Estrogen Binding ◽

Disease Free

Frozen tumor sections and/or cytological smears and aspirates from 614 pateints were studied utilizing the fluorescent estrogen binding technique of Lee. Survival analyses of the results indicated that women less than 55 years of age and with positive tumors had a statistically better disease-free interval and survived significantly longer than older patients. The method is thus an independent predictor of survival and disease-free interval in this siubgroup of breast cancer patients.

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Increased Expression of Dachshund Homolog 1 in Ovarian Cancer as a Predictor for Poor Outcome

International Journal of Gynecological Cancer ◽

10.1097/igc.0b013e31824311e6 ◽

2012 ◽

Vol 22 (3) ◽

pp. 386-393 ◽

Cited By ~ 10

Author(s):

Fei Liang ◽

Qingtao Lü ◽

Shuyu Sun ◽

Jie Zhou ◽

Vladimir M. Popov ◽

...

Keyword(s):

Ovarian Cancer ◽

Estrogen Receptor ◽

Immunohistochemical Staining ◽

Normal Tissue ◽

Prognostic Value ◽

Functional Relationship ◽

Estrogen Receptor Α ◽

Cancer Growth ◽

Protein Levels

ObjectiveThis study aimed to determine the functional relationship between the levels of dachshund homolog 1 (DACH1) expression and different subtypes of ovarian cancer and to investigate the possible prognostic value of DACH1 in ovarian cancer.MethodsImmunohistochemical staining was deployed to determine the protein levels of DACH1. Staining was performed on patient samples, for whom the detailed follow-up data have been acquired during the last 10 years. Normal, benign, borderline, cancer, and metastatic ovarian cancer samples were included in this study.ResultsThe results of our study show that DACH1 protein levels increase with the invasiveness of the ovarian cancer. As the cancer progresses from benign and borderline to metastatic, DACH1 protein expression increases as well. Moreover, with the increase in expression, the subcellular distribution of DACH1 changes from nucleus in normal tissue to cytoplasm in cancer. Finally, DACH1 expression levels were compared with estrogen receptor α (ERα) levels, and the results showed that overall DACH1 levels were higher, whereas also DACH1 exhibited increased cytoplasmic expression in ERα-positive ovarian cancer samples.ConclusionsThese results indicate that DACH1 is highly expressed in metastatic ovarian cancer compared with that of normal, benign, and borderline ovarian tissues and that it could play an important role in cancer growth.

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