Lanthanide complexes of aminophosphonates as shift reagents for 7Li and 23Na NMR studies in biological systems

Biochimie ◽  
1992 ◽  
Vol 74 (9-10) ◽  
pp. 777-783 ◽  
Author(s):  
R. Ramasamy ◽  
M.M.C.A. Castro ◽  
D.M. de Freitas ◽  
C.F.G.C. Geraldes
Keyword(s):  
Lanthanide Complexes ◽  
Biological Systems ◽  
Nmr Studies ◽  
Shift Reagents ◽  
23Na Nmr

High-resolution 23Na-NMR studies of human erythrocytes: use of aqueous shift reagents

1984 ◽  
Vol 246 (5) ◽  
pp. C528-C536 ◽  
Author(s):  
M. M. Pike ◽  
E. T. Fossel ◽  
T. W. Smith ◽  
C. S. Springer
Keyword(s):  
Human Erythrocytes ◽  
The Body ◽  
Nmr Studies ◽  
Extracellular Signals ◽  
Absolute Intensities ◽  
Lanthanide Chelates ◽  
Flame Emission ◽  
Shift Reagents ◽  
Nmr Signals ◽  
23Na Nmr

Aqueous shift reagents were used to clearly distinguish intra-and extracellular 23Na-nuclear magnetic resonance (NMR) signals in samples consisting of whole blood or suspensions of washed human erythrocytes (both fresh and outdated). The lanthanide chelates Dy(PPP)2(7-) and Tm( TTHA )3- were used to shift the extracellular signals upfield, and Dy( TTHA )3- and Tm(PPP)2(7-) were similarly used to shift extracellular resonances downfield. The absolute intensities of the signals were used along with the measured hematocrit to simultaneously determine the intra- and extracellular Na+ concentrations. The results were generally within 5% of the values determined by more time-consuming centrifugation-flame emission photometry measurements on the same samples. Thus the 23Na-NMR signals from both intra- and extracellular cations suffer no NMR invisibility within experimental error. The lower level of intracellular Na+ in fresh erythrocytes (less than 12 mM) is easily distinguished from the higher level (approximately 30 mM) in erythrocytes that have been stored (in the cold) outside the body for some weeks.

NMR studies of ion binding in biological systems

1987 ◽  
Vol 19 (1-2) ◽  
pp. 83-114 ◽  
Author(s):  
Sture Forsén ◽  
Torbjörn Drakenberg ◽  
Håkan Wennerström
Keyword(s):  
Inorganic Compounds ◽  
Biological Systems ◽  
Life Forms ◽  
Ion Binding ◽  
Nmr Studies

Biological systems must have evolved in an interplay between a great many organic and inorganic compounds. As a result a considerable number of elements - estimates range between 25 and 30 - are essential for higher life forms such as animals and man (Underwood, 1977; Williams, 1983, 1984).

NMR Studies of Drugs. 5-Methyl-5-phenylhydantoin. Applications of Lanthanide Shift Reagents in Polar Solvents to a Non-Chelating Substrate

1993 ◽  
Vol 26 (3) ◽  
pp. 411-430 ◽  
Author(s):  
Robert Rothchild ◽  
Shari Braddock ◽  
Stacey Traviglia ◽  
Kunisi S. Venkatasubban
Keyword(s):  
Nmr Studies ◽  
Polar Solvents ◽  
Lanthanide Shift Reagents ◽  
Shift Reagents

Interaction of alkaline metal ions with Ca2+-binding sites of Ca2+ -ATPase of sarcoplasmic reticulum: 23Na-NMR studies

1991 ◽  
Vol 1066 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Igor M. Timonin ◽  
Sergei N. Dvoryantsev ◽  
Victor V. Petrov ◽  
Enno K. Ruuge ◽  
Dmitri O. Levitslky
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Metal Ions ◽  
Binding Sites ◽  
Alkaline Metal ◽  
Nmr Studies ◽  
23Na Nmr
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