scholarly journals The effect of eyestalk ablation on growth, haemolymph composition and gill Na+, K+-ATPase activity of Penaeus monodon juveniles

1993 ◽  
Vol 106 (4) ◽  
pp. 621-626 ◽  
Author(s):  
Nan Fan-Hua ◽  
Sheen Shyn-Shin ◽  
Liu Ping-Chung ◽  
Shiu-Nan Chen
Keyword(s):  
Atpase Activity ◽  
Penaeus Monodon ◽  
Eyestalk Ablation

Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Reproduction ◽  
2018 ◽  
Author(s):  
Pacharawan Deenarn ◽  
Punsa Tobwor ◽  
Rungnapa Leelatanawit ◽  
Somjai Wongtriphop ◽  
Jutatip Khudet ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ovarian Development ◽  
Penaeus Monodon ◽  
Chain Fatty Acid ◽  
Regulatory Genes ◽  
Ovarian Maturation ◽  
Long Chain Fatty Acid ◽  
Eyestalk Ablation ◽  
Regulatory Pathways ◽  
Expression Levels

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

Influence of pH on the ATPase Activity and Solubility of Macrobrachium rosenbergii and Penaeus monodon Muscle Myofibrillar Protein

2005 ◽  
Vol 8 (7) ◽  
pp. 982-988
Author(s):  
Md. Kamal . ◽  
Md. Moshiur Rahman . ◽  
Mohammad Abu Jafor B . ◽  
Lubna Yasmin . ◽  
Md. Nurullah .
Keyword(s):  
Atpase Activity ◽  
Penaeus Monodon ◽  
Macrobrachium Rosenbergii ◽  
Myofibrillar Protein ◽  
Influence Of Ph

scholarly journals SALMON GONADOTROPIN RELEASING HORMONE ANALOGUE STIMULASI PEMATANGAN SPERMATOFOR UDANG WINDU (Penaeus monodon) APKIRAN TANPA ABLASI

2018 ◽  
Vol 13 (2) ◽  
pp. 67
Author(s):  
Ike Trismawanti ◽  
Agus Nawang ◽  
Asda Laining
Keyword(s):  
Penaeus Monodon ◽  
The Body ◽  
Optimum Dose ◽  
Hormone Analogue ◽  
Eyestalk Ablation ◽  
Releasing Hormone ◽  
Second Stage ◽  
Short Period ◽  
R Value ◽  
Number Of Males

Penggunaan induk pada pembenihan udang windu pada dasarnya hanya untuk periode yang singkat dan selanjutnya induk udang windu diapkir baik betina maupun jantan. Tujuan penelitian ini untuk mengevaluasi efek injeksi hormon kombinasi salmon gonadotrophin releasing hormone analogue (sGnRH-a) dan domperidone terhadap karakteristik spermatofor induk udang windu, Penaeus monodon yang telah diapkir dari unit pembenihan tanpa ablasi tangkai mata. Dua tahap penelitian dilakukan pada udang windu jantan yang diapkir dari unit pembenihan. Tahap pertama terdiri atas dua dosis 0,1 (OV-1) dan 0,2 (OV-2) mL/kg bobot udang dan ablasi mata (AB) sebagai kontrol. Pada tahap ke-2, dosis yang diaplikasikan adalah dosis terbaik dari tahap ke-1 (OV-3) dan ablasi sebagai kontrol (AB). Induk jantan apkir ditebar masing-masing dengan kepadatan 14 ekor untuk tahap ke-1 dan 12 ekor pada tahap kedua. Injeksi dilakukan tiga kali dengan interval satu minggu untuk kedua percobaan tersebut. Seminggu setelah suntikan terakhir, induk jantan diberi kejutan listrik untuk mengeluarkan spermatofornya. Pada percobaan pertama, jumlah induk jantan yang mengeluarkan spermatofor pada maturasi dan rematurasi pertama lebih banyak ditemukan pada perlakuan OV-2 daripada OV-1 dan AB. Induk jantan pada perlakuan OV-3 pada tahap ke-2 menghasilkan lebih banyak spermatofor (58,3%) dibandingkan dengan AB (50%). Hubungan antara bobot badan dan bobot spermatofor untuk total data pada tahap ke-1 sifatnya linier dengan nilai r= 0,74 sedangkan hubungan antara bobot spermatofor dan jumlah spermatozoa sifatnya tidak linier dengan nilai r= 0.14. Induk apkiran jantan udang windu masih dapat memproduksi spermatofor melalui stimulasi injeksi hormon sGnRH-a pada dosis 0,2 mL/kg bobot udang relatif lebih banyak dibandingkan ablasi.Broodstocks in black tiger hatcheries are commonly used for only a short period of time. Both female and male broodstocks are discarded due to reduced spawning frequencies. This recent study was an effort to explore whether discarded broodstocks could be re-maturated without eyestalk ablation. For the research purpose, a two-stage experiment was conducted on discarded male tiger shrimps to evaluate the effect of sGnRH-a injection on the shrimp reproductive performances. In the first stage, the experiment consisted of injection treatment of different doses of sGnRH-a at 0.1 (OV-1) and 0.2 (OV-2) mL/kg and eyestalk ablation (AB) as a control. For the second stage, the treatment consisted of injection of sGnRH-a optimum dose obtained from the first trial (0.2 mL/kg shrimp (OV-3) and ablation as the control (AB). The treated male stocks were then randomly placed in concrete tanks with a density of 14 males/tank for the first stage and 12 males for the second stage. The injections were carried-out three times with an interval of one week for both stages. A week after the last injection, males were electrically shocked to release their spermatophores. In the first stage, the number of males releasing spermatophores at maturation and first re-maturation was higher in OV-2 treatment than that of OV-1 and AB treatments. In the second trial, the number of males in OV-3 treatment released more spermatophore (58.3%) compared to AB which was 50%. The body and spermatophore weights correlation of the discarded tiger shrimp in the first trial followed a positive linear with an r-value of 0.74. However, the spermatophore weight and the sperm cell number was not positively correlated with an r-value of 0.14. The discarded male tiger shrimps produced a relatively higher spermatophore when induced with sGnRHa at a dose of 0.2 mL/kg than those of shrimp treated with ablation.

scholarly journals Gonadal maturation, fecundity and hatching performance of wild caught tiger shrimp Penaeus monodon using unilateral eyestalk ablation in captivity

2016 ◽  
Vol 13 (2) ◽  
pp. 315-322 ◽  
Author(s):  
SA Uddin ◽  
MM Rahman
Keyword(s):  
Significant Positive Correlation ◽  
Penaeus Monodon ◽  
Hatching Rate ◽  
Environmental Condition ◽  
Eyestalk Ablation ◽  
Multiple Spawning ◽  
In Captivity ◽  
Investigation Period ◽  
Moulting Cycle ◽  
Average Fertility

In this study, the effects of eyestalk ablation on maturation, moulting, spawning and hatching rate of wild caught Penaeus monodon were investigated. Twenty males (M) and 30 eyestalk–ablated and 30 non–ablated females (F), all individually marked (M: F=1:3) were stocked into two 15–ton capacity (each) maturation tank. All the ablated females moulted in 7.92±0.24 (mean±standard error) days, and first spawning occurred 3.2±0.20 days following eyestalk ablation. While twenty-five ablated females spawned, producing an average of 296,160±26,589 eggs, only eight non–ablated females spawned, producing an average of 195,462±20,565 eggs during the investigation period of 120 days. A significant positive correlation between fecundity and female body weight (P<0.05; P<0.001) was observed. Throughout the experiment, the average number of spawning per female was 1.8±0.14 for ablated and one for non–ablated females. Multiple spawning (up to three times) occurred within the same moulting cycle in ablated females. Among the ablated P. monodon females, 36% spawn once, 48% second time, and 16% third time. The average fertility rate of the eggs was high, ranging between 80 and 90% in non-ablated females, while in ablated females; the range was between 72 and 88% and differ significantly (p<0.05) ablated and non ablated females. Average hatching rate ranges between 70 and 80% for ablated and 75 and 82% for non-ablated females (p<0.05). From eggs to nauplii production per female was 167,838 for ablated and 127,500for non–ablated females. This study indicates that eyestalk ablation and environmental condition were important inducing tools for re-maturation of spent P. monodon that continued supply of seed stock for effective commercial shrimp farming.J. Bangladesh Agril. Univ. 13(2): 315-322, December 2015

scholarly journals Polychaete consumption increased prostaglandin biosynthesis in female Penaeus monodon

Reproduction ◽  
2020 ◽  
Vol 160 (6) ◽  
pp. 873-885
Author(s):  
Pacharawan Deenarn ◽  
Punsa Tobwor ◽  
Vanicha Vichai ◽  
Suwanchai Phomklad ◽  
Panomkorn Chaitongsakul ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ovarian Development ◽  
Penaeus Monodon ◽  
Prostaglandin E ◽  
Prostaglandin Biosynthesis ◽  
Eyestalk Ablation ◽  
Regulatory Pathways ◽  
Feed Pellets ◽  
Prostaglandin F ◽  
The Impact

The polychaete Perinereis nuntia is preferred over commercial feed pellets for boosting ovarian maturation of the female black tiger shrimp Penaeus monodon. High levels of prostaglandins in polychaetes are believed to enhance shrimp ovarian development. However, the impact of polychaete feeding on shrimp prostaglandin biosynthesis and fatty acid regulatory pathways have yet to be investigated. As polychaetes contain higher levels of arachidonic acid (ARA), eicosapentaenoic acid (EPA), prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) than feed pellets, we examined the effects of polychaete feeding alone and in combination with eyestalk ablation on shrimp hepatopancreases and ovaries. Shrimp fed with polychaetes contained higher levels of EPA, PGE2 and PGF2α in hepatopancreases than those of pellet-fed shrimp. Similarly, higher levels of ARA and higher transcription levels of cyclooxygenase (COX) and prostaglandin F synthase (PGFS) were detected in ovaries of polychaete-fed shrimp compared to those of pellet-fed shrimp. The combination of polychaete-feeding and eyestalk ablation, commonly practiced to induce ovarian development, increased levels of ARA and EPA and transcription levels of COX in hepatopancreases and ovaries of polychaete-fed shrimp compared to those of pellet-fed shrimp. In ovaries, prostaglandin biosynthesis gene transcripts were induced by polychaete feeding while transcriptional levels of fatty acid regulatory genes were regulated by shrimp feed and eyestalk ablation. Our findings not only elucidate the effects of polychaete consumption on shrimp prostaglandin biosynthesis and fatty acid regulatory pathways during larvae production, but also suggests that high levels of dietary ARA, EPA and prostaglandins are essential during P. monodon ovarian development.

scholarly journals Comparison of ovarian maturation and spawning after unilateral eyestalk ablation of wild-caught and pond-reared Penaeus monodon

2015 ◽  
Vol 13 (3) ◽  
pp. e0402 ◽  
Author(s):  
Weigeng Wen ◽  
Qibin Yang ◽  
Zhenhua Ma ◽  
Shigui Jiang ◽  
Lihua Qiu ◽  
...  
Keyword(s):  
Body Weight ◽  
Egg Production ◽  
Penaeus Monodon ◽  
Size Group ◽  
Hatching Rate ◽  
Weight Class ◽  
Ovarian Maturation ◽  
Egg Hatching ◽  
Eyestalk Ablation ◽  
Spawning Time

<p>The present study compares the efficiency of ovarian maturation and spawning success between wild-caught and pond-reared <em>Penaeus monodon </em>females after unilateral eyestalk ablation. The earliest spawning time after eyestalk ablation was 5.9 days in wild-caught females, which is significantly shorter than the spawning time in pond-reared females (10.5 days). Both wild-caught and pond-reared females repeatedly spawned after eyestalk ablation. On average, each wild-caught female spawned 2.94 times while each pond-reared female spawned only 1.09 times. The spawning induction rate, egg hatching rate, and the number of eggs per spawning were significantly greater in wild-caught females than in pond-reared females. However, the egg size was not significantly different between wild-caught and pond-reared females. Four shrimp sizes (60, 80, 100 and 120 (± 1.0) g) were tested in this study and body weight significantly affected ovarian induction in pond-reared females but not in wild-caught females. Within the same body-weight class, the egg number per spawn in wild-caught females was significantly greater than that in pond-reared females. The egg production per spawn of the pond-reared females in the 120-g size group was two times higher than that in the pond-reared females in the 80-g size group. In conclusion, the fecundity of wild-caught <em>P. monodon</em> females is significantly higher than that of pond-reared <em>P. monodon</em> females. In breeding pond-reared <em>P. monodon</em>, the recommended minimum body weight of females is over 80 g, and the desirable body weight is over 100 g.</p>

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