Comparative study of the symbiotic plasmid DNA in free living bacteria and bacteroids of Rhizobium leguminosarum

1989 ◽  
Vol 60 (1) ◽  
pp. 15-19
Author(s):  
C Vierny
Keyword(s):  
Comparative Study ◽  
Plasmid Dna ◽  
Rhizobium Leguminosarum ◽  
Free Living ◽  
Symbiotic Plasmid ◽  
Living Bacteria

scholarly journals A Comparative Study of Phase States of the Peribacteroid Membrane from Yellow Lupin and Broad Bean Nodules

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Natalia N. Kudryavtseva ◽  
Alexis V. Sof’in ◽  
Georgiy S. Bobylev ◽  
Evgeny M. Sorokin
Keyword(s):  
Comparative Study ◽  
Outer Membrane ◽  
Lipid Bilayer ◽  
Rhizobium Leguminosarum ◽  
Broad Bean ◽  
Lupinus Luteus ◽  
Bilayer Structure ◽  
Peribacteroid Membrane ◽  
Free Living ◽  
Yellow Lupin

A comparative study of the lipid bilayer phase status and structure of the outer membrane of free-livingBradyrhizobiumstrain 359a (Nod+Fix+) and 400 (Nod+FixL) orRhizobium leguminosarum97 (Nod+Fix+, effective) and 87 (Nod+FixL, ineffective) has been carried out. Also, the effect of the symbiotic pair combination on the lipid bilayer structure of the bacteroid outer membrane and peribacteroid membrane, isolated from the nodules ofLupinus luteusL. orVicia fabaL., has been studied. As a result, it is shown that the lipid bilayer status of the bacteroid outer membrane is mainly determined by microsymbiont, but not the host plant. In the contrast, the lipid bilayer status of the peribacteroid membrane and, as a consequence, its properties depend on interaction of both symbiotic partners.

Unusual localization of nod and nif genes in Rhizobium leguminosarum bv. viciae

1997 ◽  
Vol 43 (4) ◽  
pp. 399-402 ◽  
Author(s):  
Sylvie-Isabelle Mazurier ◽  
Gisele Laguerre
Keyword(s):  
Plasmid Dna ◽  
Genomic Dna ◽  
Rhizobium Leguminosarum ◽  
Large Plasmid ◽  
Nif Genes ◽  
Symbiotic Genes ◽  
Nod Genes ◽  
Symbiotic Plasmid ◽  
Wild Strains ◽  
Sym Plasmid

Genomic DNA from seven strains of Rhizobium leguminosarum bv. viciae isolated from nodules of field-grown lentils showed homology to nod and nif gene probes, whereas plasmid DNA did not hybridize with these probes. The results suggest that symbiotic genes could be located on the chromosome or perhaps on a very large plasmid that could not be resolved in Eckhardt gels. Each strain contained one plasmid that hybridized with a pSym isolated from a R. leguminosarum strain of the same field population. This finding led us to hypothesize that the nod and nif genes of the seven strains might have originated from a Sym plasmid and have been integrated into another replicon. The ability to nodulate vetch was confirmed for all of the seven strains. Thus, wild strains of R. leguminosarum bv. viciae that nodulate vetch carry nod and nif genes either on the chromosome or on an extrachromosomal replicon of size much larger than the pSyms hitherto described.Key words: Rhizobium leguminosarum, nod genes, nif genes, chromosome, symbiotic plasmid, megaplasmid.

scholarly journals A sulfoglycolytic Entner-Doudoroff pathway in Rhizobium leguminosarum bv. trifolii SRDI565

2019 ◽  
Author(s):  
Jinling Li ◽  
Ruwan Epa ◽  
Nichollas E. Scott ◽  
Dominic Skoneczny ◽  
Mahima Sharma ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Rhizobium Leguminosarum ◽  
Glycolytic Enzyme ◽  
Comparative Proteomics ◽  
Aldehyde Reductase ◽  
Proteomics Data ◽  
Free Living ◽  
Symbiotic Relationships ◽  
Living Bacteria ◽  
Metabolomics Analysis

AbstractRhizobia are nitrogen fixing bacteria that engage in symbiotic relationships with plant hosts but can also persist as free-living bacteria with the soil and rhizosphere. Here we show that free living Rhizobium leguminosarum SRDI565 can grow on the sulfosugar sulfoquinovose (SQ) using a sulfoglycolytic Entner-Doudoroff (sulfo-ED) pathway resulting in production of sulfolactate (SL) as the major metabolic end-product. Comparative proteomics supports the involvement of a sulfo-ED operon encoding an ABC transporter cassette, sulfo-ED enzymes and an SL exporter. Consistent with an oligotrophic lifestyle, proteomics data revealed little change in expression of the sulfo-ED proteins during growth on SQ versus mannitol, a result confirmed through biochemical assay of sulfoquinovosidase activity in cell lysates (data are available via ProteomeXchange with identifier PXD015822). Metabolomics analysis showed that growth on SQ involves gluconeogenesis to satisfy metabolic requirements for glucose-6-phosphate and fructose-6-phosphate. Metabolomics analysis also revealed the unexpected production of small amounts of sulfofructose and 2,3-dihydroxypropanesulfonate, which are proposed to arise from promiscuous activities of the glycolytic enzyme phosphoglucose isomerase and a non-specific aldehyde reductase, respectively. This work shows that rhizobial metabolism of the abundant sulfosugar SQ may contribute to persistence of the bacteria in the soil and to mobilization of sulfur in the pedosphere.

Conformity and diversity among field isolates of Rhizobium leguminosarum bv. viciae, bv. trifolii, and bv. phaseoli revealed by DNA hybridization using chromosome and plasmid probes

1993 ◽  
Vol 39 (4) ◽  
pp. 412-419 ◽  
Author(s):  
Gisèle Laguerre ◽  
Eric Geniaux ◽  
Sylvie Isabelle Mazurier ◽  
Raquel Rodriguez Casartelli ◽  
Noëlle Amarger
Keyword(s):  
Host Plant ◽  
Plant Species ◽  
Plasmid Dna ◽  
Dna Hybridization ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Plant Host ◽  
Host Plant Species ◽  
Symbiotic Plasmid ◽  
Chromosomal Type

A study was made of 113 bacterial isolates from root nodules of peas, lentils, red clover, and French beans, which had been grown in the same soil. Plasmid band profiles visualized in Eckhardt gels were analysed in relation to DNA hybridization patterns obtained by probing restricted total cellular DNA in Southern blots. Rhizobium leguminosarum chromosomal probes (plac12, pCOS309.1) and various symbiotic plasmid (nod gene region) probes were used. Dominant plasmid DNA hybridization patterns and more frequent combinations of plasmid patterns and chromosomal types were found among the isolates of each host plant species; the occurrence of alternative combinations indicated that genetic transfer and recombination among members of this soil population had taken place. About 40% of all isolates belonged to the same chromosomal type. Isolates of the same chromosomal type were often found with cryptic plasmids of the same size in different host plant species. Although isolates could not be assigned to their respective plant host groups using chromosomal probes alone, this was generally possible using symbiotic plasmid probes and the results were in complete accordance with plant tests. However, there was a group of bean isolates in which no homology to any of the R. leguminosarum probes was detected under the conditions of high stringency used. Other exceptional isolates of beans conformed in probe tests and subsequent plant host specificity tests better to biovars viciae or trifolii than to biovar phaseoli; thus, the nodulation of beans (i.e., Phaseolus vulgaris) in the field appears less subject to stringent control of specificity than that of other host plant species. It was also noted that the nod gene regions probed showed greater diversity in isolates of biovars viciae and trifolii than in biovar phaseoli.Key words: Rhizobium leguminosarum, genetic diversity, plasmid, DNA hybridization, restriction fragment length polymorphism.

Contribution of the Symbiotic Plasmid to the Competitiveness of Rhizobium leguminosarum

Microbiology ◽  
1983 ◽  
Vol 129 (10) ◽  
pp. 2973-2977 ◽  
Author(s):  
N. J. Brewin ◽  
E. A. Wood ◽  
J. P. W. Young
Keyword(s):  
Rhizobium Leguminosarum ◽  
Symbiotic Plasmid

Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction

2016 ◽  
Vol 513 ◽  
pp. 68-76 ◽  
Author(s):  
H. Rahnama ◽  
A. Sattarzadeh ◽  
F. Kazemi ◽  
N. Ahmadi ◽  
F. Sanjarian ◽  
...  
Keyword(s):  
Comparative Study ◽  
Dna Extraction ◽  
Plasmid Dna ◽  
Nano Particles ◽  
Magnetic Nano Particles

scholarly journals Comparative Study on Antioxidant Capacity of Self-fermenting Enzyme and Commercial Enzyme

2021 ◽  
Vol 251 ◽  
pp. 02032
Author(s):  
Fenghong Liu ◽  
Xianhao Cheng ◽  
Jing Miu ◽  
Xiaotong Cui ◽  
Xiaojuan Gao ◽  
...  
Keyword(s):  
Free Radicals ◽  
Comparative Study ◽  
Antioxidant Capacity ◽  
Hydroxyl Radicals ◽  
Superoxide Anion ◽  
Antioxidant Ability ◽  
Commercial Enzyme ◽  
Visible Spectrophotometry ◽  
Uv Visible ◽  
Living Bacteria

In order to provide consumers with a more reasonable method of using Enzyme, UV-visible spectrophotometry was used to study the antioxidant ability of self-fermenting Enzyme and commercial Enzyme. By measuring the ability of fourteen kinds of self-fermenting Enzyme and three commercial Enzyme to scavenge DPPH free radicals, superoxide anion and hydroxyl radicals and the number of living bacteria, the following conclusions were drawn: (1) Commercial Enzyme has more properties than self-fermenting Enzyme of antioxidant capacity; (2) Compound fruit Enzyme has stronger antioxidant ability than single fruit Enzyme; (3) Self-fermenting Enzyme is not sterilized including more living bacteria; (4) Commercial Enzyme has a higher drinking value than self-fermenting Enzyme.

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