Effects of ochratoxins A and B on prechondrogenic mesenchymal cells from chick embryo limb buds

1990 ◽  
Vol 54 (2-3) ◽  
pp. 129-134 ◽  
Author(s):  
Richard Wiger ◽  
Fredrik C. Størnier
Keyword(s):  
Chick Embryo ◽  
Mesenchymal Cells ◽  
Limb Buds

Extracellular matrix synthesis in the chick embryo lateral plate prior to and during limb outgrowth

Development ◽  
1980 ◽  
Vol 59 (1) ◽  
pp. 71-87
Author(s):  
Trent D. Stephens ◽  
N. S. Vasan ◽  
James W. Lash
Keyword(s):  
Extracellular Matrix ◽  
Chick Embryo ◽  
Molecular Basis ◽  
Limb Bud ◽  
Matrix Synthesis ◽  
Lateral Plate ◽  
Limb Buds ◽  
Cartilage Development ◽  
Limb Outgrowth ◽  
Lateral Plates

Little is known at the present time about the molecular basis and mechanisms of morphogenesis. The present study is an attempt to determine what influence the extracellular matrix has on the initial outgrowth of the limb bud. Stage -12 to -18 chick embryo lateral plates were examined in relation to proline and sulfate incorporation into collagen and proteoglycan. The flank and limbs incorporated the same amount of labeled proline and sulfate before stage 16. At stage 16 the flank began to incorporate more of both isotopes until at stage 18 there was twice as much incorporation into the flank as into the limbs. The flank and limbs contained the same type of collagen during the period examined. The limbs contained both large and small proteoglycans but the flank contained only small proteoglycans. These data suggest that the extracellular matrix in the flank and limb regions may play a role in limb outgrowth and that the limb buds at these stages may be more inclined toward cartilage development.

The distribution of the polarizing zone (ZPA) in the legbud of the chick embryo.

Development ◽  
1985 ◽  
Vol 86 (1) ◽  
pp. 169-175
Author(s):  
J. Richard Hinchliffe ◽  
Anna Sansom
Keyword(s):  
Pattern Formation ◽  
Chick Embryo ◽  
High Activity ◽  
Posterior Margin ◽  
Posterior Part ◽  
Limb Bud ◽  
Limb Buds ◽  
Anteroposterior Axis ◽  
Low Activity

The stage-21 to 22 legbud polarizing zone (ZPA) was mapped by transplanting small blocks of posterior marginal mesenchyme preaxially into stage-20 to -22 chick wing buds and assessing the degree of duplication of the wing digital skeleton produced in the host. Blocks taken from the posterior flank, from the angle between posterior flank and the proximal base of the limb bud, and from the most anterior distal position chosen (under the AER), all had very low activity. Blocks taken from the posterior margin of the legbud, plus the next distal block under the posterior part of the AER, all had high activity. We consider that barrier and amputation results on wing and legbud, when interpreted in the light of maps of the ZPA in both limb buds, are consistent with the hypothesis that both leg and wing have their growth and anteroposterior axis of pattern formation controlled by the ZPA.

Stage differential effects of verteporfin on the differentiation of chick embryo wing bud mesenchymal cells

Biologia ◽  
2019 ◽  
Vol 74 (9) ◽  
pp. 1219-1228
Author(s):  
Bohyeon Jeong ◽  
DongHyun Kim ◽  
Juhee Kim ◽  
Jong Kyung Sonn
Keyword(s):  
Chick Embryo ◽  
Mesenchymal Cells ◽  
Differential Effects ◽  
Wing Bud

scholarly journals STRUCTURAL VARIATIONS DURING MITOSIS IN THE CHICK EMBRYO

1967 ◽  
Vol 33 (1) ◽  
pp. 179-196 ◽  
Author(s):  
Allan L. Allenspach ◽  
L. E. Roth
Keyword(s):  
Free Surface ◽  
Cell Division ◽  
Epithelial Cells ◽  
Chick Embryo ◽  
Mesenchymal Cells ◽  
Cleavage Furrow ◽  
Chick Embryos ◽  
Structural Variations ◽  
Spindle Elongation ◽  
Elongation Process

Selected tissues from chick embryos were fixed in 2% glutaraldehyde and 1% OsO4, both buffered at pH 7.6 with Veronal-acetate, and were embedded in Maraglas or Araldite. Two types of cell division have been noted. Generally, epithelial cells divide predominantly by a shortening of the chromosome-to-pole distance rather than by spindle elongation; mesenchymal cells undergo extensive spindle elongation. The presence of numerous continuous microtubules in cells that undergo extensive spindle elongation functionally implicates these tubules in the elongation process. In most embryonic epithelia, the cleavage furrow converges to a fixed site forming a mid-body near the anchoring desmosomes at the free surface; symmetrical furrow formation is typical of mesenchymal cells which lack desmosomes. The hypothesis of cleavage furrow formation and the fate of the mid-body that is formed during cytokinesis are discussed.

Evidence that the nerve controls molecular identity of progenitor cells for limb regeneration

Development ◽  
1988 ◽  
Vol 103 (3) ◽  
pp. 567-573
Author(s):  
D.M. Fekete ◽  
J.P. Brockes
Keyword(s):  
Monoclonal Antibody ◽  
Progenitor Cells ◽  
Limb Regeneration ◽  
Growth Zone ◽  
Mesenchymal Cells ◽  
Limb Bud ◽  
Limb Buds ◽  
Molecular Identity

Adult urodele amphibians can regenerate their limbs after amputation by a process that requires the presence of axons at the amputation plane. Paradoxically, if the limb develops in the near absence of nerves (the ‘aneurogenic’ limb) it can subsequently regenerate in a nerve-independent fashion. The growth zone (blastema) of regenerating limbs normally contains progenitor cells whose division is nerve-dependent. A monoclonal antibody that marks these nerve-dependent cells in the normal blastema does not stain the mesenchymal cells of developing limb buds and only stains the amputated limb bud when axons have reached the plane of amputation. This report shows that the blastemal cells of the regenerating aneurogenic limb also fail to react with the antibody in situ. These data suggest that the blastemal cells arising during normal regeneration have been altered by the nerve. This regulation may occur either at the time of amputation (when the antigen is expressed) or during development (when the limb is first innervated).

scholarly journals Observation of the abluminal surface of the atrioventricular endothelium which undergoes transition into cardiac cushion mesenchymal cells in the chick embryo.

2000 ◽  
Vol 42 (3) ◽  
pp. 169-175
Author(s):  
Keiko Iwakami ◽  
Keitaro Isokawa ◽  
Hitomi Sejima ◽  
Osamu Shimizu ◽  
Tamotsu Morikawa ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Mesenchymal Cells ◽  
Cardiac Cushion

Expression and function of Ebf1 gene during chondrogenesis in chick embryo limb buds

Gene ◽  
2021 ◽  
pp. 145895
Author(s):  
Mohammed Abu EL-Magd ◽  
Ahmed Abdelfattah-Hassan ◽  
Rasha A. Elsisy ◽  
Yousef M Hawsawi ◽  
Atif A. Oyouni ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Limb Buds ◽  
And Function

scholarly journals Reconstruction of distinct vertebrate gastrulation modes via modulation of key cell behaviours in the chick embryo

2021 ◽  
Author(s):  
Manli Chuai ◽  
Guillermo Serrano Nájera ◽  
Mattia Serra ◽  
L. Mahadevan ◽  
Cornelis J. Weijer
Keyword(s):  
Chick Embryo ◽  
Teleost Fish ◽  
Primitive Streak ◽  
Theoretical Framework ◽  
Mesenchymal Cells ◽  
Signalling Pathways ◽  
Vertebrate Species ◽  
Self Organized ◽  
Cellular Behaviors ◽  
Epithelial Sheets

The morphology of gastrulation driving the internalisation of the mesoderm and endoderm differs dramatically among vertebrate species. It ranges from involution of epithelial sheets of cells through a circular blastopore in amphibians to ingression of mesenchymal cells through a primitive streak in amniotes. By targeting signalling pathways controlling critical cell behaviours in the chick embryo, we generated crescent- and ring-shaped mesendoderm territories in which cells can or cannot ingress. These alterations subvert the formation of the chick primitive streak into the gastrulation modes seen in amphibians, reptiles and teleost fish. Our experimental manipulations are supported by a theoretical framework linking cellular behaviors to self-organized multi-cellular flows in the accompanying paper. All together, this suggests that the evolution of gastrulation movements are largely determined by the shape of and cell behaviours in the mesendoderm territory across different species, and controlled by a relatively small number of signalling pathways.

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