Simultaneous analysis of azidothymidine and its mono-, di- and triphosphate derivatives in biological fluids, tissue and cultured cells by a rapid high-performance liquid chromatographic method

1992 ◽  
Vol 579 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Grietje Molema ◽  
Robert W. Jansen ◽  
Jan Visser ◽  
Dirk K.F. Meijer
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
Cultured Cells ◽  
Biological Fluids ◽  
High Performance Liquid Chromatographic ◽  
Simultaneous Analysis ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Triphosphate Derivatives

Simplified liquid-chromotographic analysis for cyclosporin A, and comparison with radioimmunoassay.

1983 ◽  
Vol 29 (1) ◽  
pp. 180-183 ◽  
Author(s):  
S G Carruthers ◽  
D J Freeman ◽  
J C Koegler ◽  
W Howson ◽  
P A Keown ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
High Performance ◽  
Chromatographic Method ◽  
Biological Fluids ◽  
High Performance Liquid Chromatographic ◽  
Immunosuppressive Drug ◽  
Internal Standards ◽  
Liquid Chromatographic Method ◽  
The Difference ◽  
Liquid Chromatographic

Abstract We describe a simplified isocratic "high-performance" liquid-chromatographic method for measuring a new immunosuppressive drug, cyclosporin A, in biological fluids with use of its analogs cyclosporin C and cyclosporin D as internal standards. The method is reproducible and accurate and appears to be specific for cyclosporin A; the detection limit is 31 micrograms/L. The chromatographic measurements of the concentration of cyclosporin A in serum of patients receiving the drug were invariably lower than those by radioimmunoassay and the difference became more pronounced the greater the period of time after dosing. Because measurements of cyclosporin A in serum standards were almost identical with both techniques, the differences between the two sets of results for patients' samples suggests that the radioimmunoassay is nonspecific and measures metabolites of cyclosporin A.

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