New high-performance liquid chromatographic method for the detection of picolinic acid in biological fluids

2001 ◽  
Vol 751 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Cecilia Dazzi ◽  
Giovanni Candiano ◽  
Stefano Massazza ◽  
Antonio Ponzetto ◽  
Luigi Varesio
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
Biological Fluids ◽  
Picolinic Acid ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Simplified liquid-chromotographic analysis for cyclosporin A, and comparison with radioimmunoassay.

1983 ◽  
Vol 29 (1) ◽  
pp. 180-183 ◽  
Author(s):  
S G Carruthers ◽  
D J Freeman ◽  
J C Koegler ◽  
W Howson ◽  
P A Keown ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
High Performance ◽  
Chromatographic Method ◽  
Biological Fluids ◽  
High Performance Liquid Chromatographic ◽  
Immunosuppressive Drug ◽  
Internal Standards ◽  
Liquid Chromatographic Method ◽  
The Difference ◽  
Liquid Chromatographic

Abstract We describe a simplified isocratic "high-performance" liquid-chromatographic method for measuring a new immunosuppressive drug, cyclosporin A, in biological fluids with use of its analogs cyclosporin C and cyclosporin D as internal standards. The method is reproducible and accurate and appears to be specific for cyclosporin A; the detection limit is 31 micrograms/L. The chromatographic measurements of the concentration of cyclosporin A in serum of patients receiving the drug were invariably lower than those by radioimmunoassay and the difference became more pronounced the greater the period of time after dosing. Because measurements of cyclosporin A in serum standards were almost identical with both techniques, the differences between the two sets of results for patients' samples suggests that the radioimmunoassay is nonspecific and measures metabolites of cyclosporin A.

Sign in / Sign up

Export Citation Format

Share Document