Nigrostriatal dopamine neurons receive substance P-ergic inputs in the substantia nigra: Application of the immunoelectron microscopic mirror technique to the fluorescent double-staining method for transmitter-specific projections

1987 ◽  
Vol 5 ◽  
pp. S149
Author(s):  
Yoshinori Kawai ◽  
Hiroshi Takagi ◽  
Yayoi Kumoi ◽  
Sadao Shiosaka ◽  
Masaya Tohyama
Keyword(s):  
Substance P ◽  
Substantia Nigra ◽  
Staining Method ◽  
Dopamine Neurons ◽  
Double Staining ◽  
Nigrostriatal Dopamine ◽  
Double Staining Method ◽  
Nigrostriatal Dopamine Neurons

scholarly journals Chemogenetic activation of nigrostriatal dopamine neurons in freely moving common marmosets

2021 ◽  
Author(s):  
Koki Mimura ◽  
Yuji Nagai ◽  
Ken-ichi Inoue ◽  
Jumpei Matsumoto ◽  
Yukiko Hori ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Positron Emission Tomography Imaging ◽  
Dopamine Neurons ◽  
Designer Drugs ◽  
Primate Model ◽  
Common Marmosets ◽  
Positron Emission ◽  
Nigrostriatal Dopamine ◽  
Nigrostriatal Dopamine Neurons

To interrogate particular neuronal pathways in non-human primates under natural and stress-free conditions, we applied designer receptors exclusively activated by designer drugs (DREADDs) technology to common marmosets. We injected adeno-associated virus vectors expressing the excitatory DREADD hM3Dq into the unilateral substantia nigra in three marmosets. Using multi-tracer positron emission tomography imaging, we detected DREADD expression in vivo, which was confirmed in nigrostriatal dopamine neurons by immunohisto-chemistry, and assessed activation of the substantia nigra and dopamine release following agonist administration. The marmosets rotated in a contralateral direction relative to the activated side 30–90 min after consuming food containing the highly potent DREADD agonist deschloroclozapine (DCZ), but not on the following days without DCZ. These results indicate that non-invasive and reversible DREADD manipulation will extend the utility of marmoset as a primate model for linking neuronal activity and natural behavior in various contexts.

Enzyme histochemical application and backscattered electron imaging to visualize the distribution of lymphatic capillaries

1990 ◽  
Vol 48 (3) ◽  
pp. 880-881
Author(s):  
Seiji Kato
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Reaction Products ◽  
Blood Capillaries ◽  
Backscattered Electron Imaging ◽  
Histochemical Observation ◽  
Backscattered Electron ◽  
Double Staining Method ◽  
Electron Imaging ◽  
Cryostat Sections

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.

scholarly journals Immunohistochemical/histochemical double staining method in the study of the columnar metaplasia of the oesophagus

2014 ◽  
Vol 58 (1) ◽  
Author(s):  
D. Cabibi ◽  
A.G. Giannone ◽  
C. Mascarella ◽  
C. Guarnotta ◽  
M. Castiglia ◽  
...  
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Columnar Metaplasia ◽  
Double Staining Method

scholarly journals Functional Crosstalk between CB and TRPV1 Receptors Protects Nigrostriatal Dopaminergic Neurons in the MPTP Model of Parkinson’s Disease

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Rayul Wi ◽  
Young Cheul Chung ◽  
Byung Kwan Jin ◽  
Lihua Duan
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Significant Loss ◽  
Glial Activation ◽  
Dopamine Neurons ◽  
Pcr Analysis ◽  
Beneficial Effects ◽  
Nigrostriatal Dopamine ◽  
Nigrostriatal Dopamine Neurons

The present study examined whether crosstalk between cannabinoid (CB) and transient potential receptor vanilloid type 1 (TRPV1) could contribute to the survival of nigrostriatal dopamine neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson’s disease (PD). MPTP induced a significant loss of nigrostriatal dopamine neurons and glial activation in the substantia nigra (SN) and striatum (STR) as visualized by tyrosine hydroxylase (TH) or macrophage antigen complex-1 (MAC-1) or glial fibrillary acidic protein (GFAP) immunocytochemistry, respectively. RT-PCR analysis shows the upregulation of inducible nitric oxide synthase, interleukin-1β, and tumor necrosis factor-α in microglia in the SN in vivo, indicating the activation of the inflammatory system. By contrast, treatment with capsaicin (a specific TRPV1 agonist) increased the survival of dopamine neurons in the SN and their fibers and dopamine levels in the STR in MPTP mice. Capsaicin neuroprotection is accompanied by inhibiting MPTP-induced glial activation and production of inflammatory cytokines. Treatment with AM251 and AM630 (CB1/2 antagonists) abolished capsaicin-induced beneficial effects, indicating the existence of a functional crosstalk between CB and TRPV1. Moreover, treatment with anandamide (an endogenous agonist for both CB and TRVP1) rescued nigrostriatal dopamine neurons and reduced gliosis-derived neuroinflammatory responses in MPTP mice. These results suggest that the cannabinoid and vanilloid system may be beneficial for the treatment of neurodegenerative diseases, such as PD, that are associated with neuroinflammation.

Sign in / Sign up

Export Citation Format

Share Document