Induction and Secretion of Hydrolytic Enzymes by the Biocontrol Agent Trichoderma Harzianum

Trichoderma harzianum is a potential biocontrol agent against Sclerotinia sclerotiorum in soybean. Information is needed on the compatibility of this biocontrol agent and the post-emergent herbicides used in soybean cropping.Haloxyfop R Methyl (EC 10.4%), Glyphosate (SL 48%), Imazamox (WG 70%) and Imazethapyr (SL 10%) were evaluated for their effects on the mycelial growth of T. harzianum on in vitro agar plates. Glyphosate (2000 ppm), Imazethapyr (500 and 250 ppm) and Haloxyfop R Methyl (1000, 500 and 100 ppm) reduced the mycelial growth of T. harzianum . Imazamox had no effect at any concentration.Subsequently, all the herbicides were assessed for their effect on soil populations of T. harzianum . Greenhouse assays conducted with non-sterile soil inoculated with T. harzianum and a specific herbicide were sampled before pesticide application and after 30 days. The number of colony forming units per gram of soil (c.f.u./g of soil) was evaluated with a soil dilution technique using Trichoderma selective medium (TSM). No detrimental effect was revealed.

Download Full-text

EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.216115-123 ◽

2017 ◽

Vol 16 (2) ◽

pp. 115

Author(s):

Johanna Taribuka ◽

Christanti Sumardiyono ◽

Siti Muslimah Widyastuti ◽

Arif Wibowo

Keyword(s):

Biological Control ◽

Phylogenetic Analysis ◽

Endophytic Fungi ◽

Trichoderma Harzianum ◽

Biocontrol Agent ◽

Biological Control Agent ◽

Control Agent ◽

Selective Medium ◽

Trichoderma Spp ◽

Its1 And Its2

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.

Download Full-text

Bio-efficacy of Trichoderma species against Pigeonpea wilt pathogen

Journal of Applied and Natural Science ◽

10.31018/jans.v9i4.1531 ◽

2017 ◽

Vol 9 (4) ◽

pp. 2327-2331 ◽

Cited By ~ 1

Author(s):

Balkishan Chaudhary ◽

Sanjeev Kumar ◽

Shiva Kant Kushwaha

Keyword(s):

Volatile Compounds ◽

Trichoderma Harzianum ◽

Mycelial Growth ◽

Biocontrol Agent ◽

Trichoderma Viride ◽

Control Agent ◽

Trichoderma Virens ◽

Trichoderma Species ◽

Fusarium Udum

Three biocontrol agent viz., Trichoderma viride, Trichoderma virens and Trichoderma harzianum were evaluated to test the antagonism against Fusarium udum under in vitro conditions. All the three biocontrol agents have the potential of parasitizing the growth of Fusarium udum in vitro. The rate of parasitism was found fastest in T. viride (61.12% over growth in 96 hrs) than T. virens and T. harzianum. The volatile compounds from Trichoderma viride suppressed the mycelial growth of Fusarium udum by 43.13% and found effective when compared to Tricho-derma virens and Trichoderma harzianum. Non-volatile compounds or culture filtrate from Trichoderma virens at 15% concentration shows complete mycelial inhibition of the test fungi. The antagonist T. virens was chosen to be the most promising bio-control agent for F. udum.

Download Full-text