Interactions Between Nutrition and Infections With Haemonchus contortus and Related Gastrointestinal Nematodes in Small Ruminants

2016 ◽  
pp. 239-351 ◽  
Author(s):  
H. Hoste ◽  
J.F.J. Torres-Acosta ◽  
J. Quijada ◽  
I. Chan-Perez ◽  
M.M. Dakheel ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Small Ruminants ◽  
Gastrointestinal Nematodes

Effect of Jatropha curcas Latex on L3 Haemonchus contortus Larval Motility

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Noorzaid Muhamad ◽  
Syahirah Sazeli ◽  
Resni Mona ◽  
Jannathul Firdous
Keyword(s):  
Larvicidal Activity ◽  
Jatropha Curcas ◽  
Haemonchus Contortus ◽  
Anthelmintic Resistance ◽  
Small Ruminants ◽  
Gastrointestinal Nematodes ◽  
Dose Dependent ◽  
Plants Extract

The anthelmintic resistance has limited the control of gastrointestinal nematodes of small ruminants and thus has awakened interest in the study of plants extract as a source of anthelmintics. These experiments were carried out to evaluate the in vitro efficacy of Jatrophacurcas latex extract against Haemonchuscontortus larval motility. To evaluate the larvicidal activity, H.contortus L3 were incubated with the extracts with varying concentration of 5 mg/mL, 10 mg/mL, 15 mg/mL and 20 mg/mL at 27°C for 48, 72 and 96 hrs. The results were subjected to the Kruskal-Wallis test (P less than 0.05). The extracts showed dose-dependent larvicidal effects. These results suggest that J.curcas can be used to control gastrointestinal nematodes of small ruminants.

scholarly journals Effect of Five Mediterranean Shrubs Extracts on Larval Exsheathment of Haemonchus contortus

2021 ◽  
Author(s):  
A. Aïssa ◽  
F. Manolaraki ◽  
H. Ben Salem ◽  
H. Hoste ◽  
K. Kraiem
Keyword(s):  
Haemonchus Contortus ◽  
Small Ruminants ◽  
Good Alternative ◽  
Gastrointestinal Nematodes ◽  
Shrub Species ◽  
Medicago Arborea ◽  
Mediterranean Shrubs ◽  
Third Stage

Background: Mediterranean shrub species cover more than 70% of the total area in Tunisia and in summer when the herbaceous species have wilted, they constitute feeding resource for livestock. The use of tanniniferous shrubs seems to be a good alternative to control gastrointestinal nematodes infections in small ruminants. This study evaluated the in vitro anthelmintic (AH) effect of Ceratonia siliqua (C. siliqua), Periploca angustifolia Labill. (P. angustifolia) and Medicago arborea (M. arborea) against Haemonchus contortus third stage larvae (L3). Methods: The larval exsheathment assay (LEA) was used to determine the proportions (%) of exsheathment of five acetonic extracts at different concentrations (1200, 600, 300, 150 μg/ml). To confirm the role of tannins in the AH effects of extracts, polyvinyl polypyrolidone (PVPP) was used as deactivating chemical tannins. Result: The highest % L3 exsheathed was recorded for M. arborea (55.01%) and the lowest value was founded for C. siliqua and P. angustifolia leaves (16.26%). Our results were concentration-dependent (P less than 0.001). The % of exsheathment increased as the time of incubation increased (P less than 0.001). P. angustifolia pods recorded the lowest EC50 value (P less than 0.05). After PVPP addition, all the acetonic extracts showed a restoration of L3 exsheathment values similar to control values (P less than 0.001).

Vaccination of sheep and cattle against haemonchosis

2015 ◽  
Vol 89 (5) ◽  
pp. 517-525 ◽  
Author(s):  
C.C. Bassetto ◽  
A.F.T. Amarante
Keyword(s):  
Haemonchus Contortus ◽  
Small Ruminants ◽  
Cysteine Proteases ◽  
Gastrointestinal Nematodes ◽  
Intestinal Cells ◽  
Antigen Vaccine ◽  
Parasitic Gastroenteritis ◽  
Native Antigen ◽  
Secretory Products ◽  
Potential Option

AbstractVaccines against gastrointestinal nematodes are one potential option for the control of parasitic gastroenteritis in ruminants. Excretory/secretory (E/S) and hidden antigens are being studied as candidates for vaccines against Haemonchus spp., which is a major parasite in cattle and small ruminants that are raised in warm climates. Protection has been observed after vaccination with some E/S proteases, particularly cysteine proteases and with some glycans that are abundant on the surfaces and in the secretory products of helminths. However, the most promising results are being obtained with glycoprotein antigens extracted from the microvillar surfaces of the Haemonchus contortus intestinal cells. These antigens are called ‘hidden′ because they are not exposed to the host's immune system during infection. Thus far, recombinant forms of these antigens have not been usefully protective. However, because only 5 μg of antigen is required per dose, production of a native antigen vaccine from adult parasites has been found to be practical and commercially viable. Trials indicate that a vaccine made from one particular isolate will cross-protect against geographically distant isolates.

Essential oils from Ocimum basilicum cultivars: analysis of their composition and determination of the effect of the major compounds on Haemonchus contortus eggs

2021 ◽  
Vol 95 ◽  
Author(s):  
A.I.P. Sousa ◽  
C.R. Silva ◽  
H.N. Costa-Júnior ◽  
N.C.S. Silva ◽  
J.A.O. Pinto ◽  
...  
Keyword(s):  
Essential Oils ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Ocimum Basilicum ◽  
Alternative Methods ◽  
Egg Hatch ◽  
Plant Essential Oils ◽  
Methyl Chavicol ◽  
The Individual

Abstract The continuous use of synthetic anthelmintics against gastrointestinal nematodes (GINs) has resulted in the increased resistance, which is why alternative methods are being sought, such as the use of natural products. Plant essential oils (EOs) have been considered as potential products for the control of GINs. However, the chemical composition and, consequently, the biological activity of EOs vary in different plant cultivars. The aim of this study was to evaluate the anthelmintic activity of EOs from cultivars of Ocimum basilicum L. and that of their major constituents against Haemonchus contortus. The EOs from 16 cultivars as well the pure compound linalool, methyl chavicol, citral and eugenol were used in the assessment of the inhibition of H. contortus egg hatch. In addition, the composition of three cultivars was simulated using a combination of the two major compounds from each. The EOs from different cultivars showed mean Inhibition Concentration (IC50) varying from 0.56 to 2.22 mg/mL. The cultivar with the highest egg-hatch inhibition, Napoletano, is constituted mainly of linalool and methyl chavicol. Among the individual compounds tested, citral was the most effective (IC50 0.30 mg/mL). The best combination of compounds was obtained with 11% eugenol plus 64% linalool (IC50 0.44 mg/mL), simulating the Italian Large Leaf (Richters) cultivar. We conclude that different cultivars of O. basilicum show different anthelmintic potential, with cultivars containing linalool and methyl chavicol being the most promising; and that citral or methyl chavicol isolated should also be considered for the development of new anthelmintic formulations.

scholarly journals Bacterial Culture Filtrates Antinematode Activity of Abomasum Bacterial Culture Filtrates against Haemonchus contortus in Small Ruminants

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1843
Author(s):  
Asfa Nazish ◽  
Fozia ◽  
Baharullah Khattak ◽  
Taj Ali Khan ◽  
Ijaz Ahmad ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Bacterial Culture ◽  
Small Ruminants ◽  
Adult Mortality ◽  
Comamonas Testosteroni ◽  
Bacterial Isolates ◽  
Egg Hatch ◽  
Nematode Parasites ◽  
Culture Filtrates ◽  
Nematode Mortality

Haemonchosis is a parasitic disease of small ruminants that adversely affects livestock production. Haemonchus contortus is one of the most prevalent nematode parasites that infect the abomasum of small ruminants. This parasite reduces milk production, overall growth and sometimes causes the death of the infected animals. The evaluation of the biocontrol potential of some abomasum bacterial isolates against H. contortus is investigated in this study. Out of which, three isolates—Comamonas testosteroni, Comamonas jiangduensis, Pseudomonas weihenstephanesis—show significant effect against the nematode L3, adult, and egg hatch inhibition assays. Various concentrations of metabolites from these bacteria are prepared and applied in different treatments compared with control. In the case of adult mortality assay, 50% metabolites of C. testosteroni and P. weihenstephanesis show 46% adult mortality, whereas C. jiangduensis shows 40% mortality. It is observed that decreasing the concentration of bacterial metabolite, lowers nematode mortality. The minimum nematode mortality rate is recorded at the lowest filtrates concentration of all the bacterial isolates. The same trend is observed in egg hatch inhibition assay, where the higher concentration of bacterial culture filtrates shows 100% inhibition of H. contortus egg. It is concluded that the effect of bacterial culture filtrates against H. contortus is dose-dependent for their activity against nematode L3, adult, and inhibition of egg hatchment.

scholarly journals Gastrointestinal nematode infections in sheep raised in Botucatu, state of São Paulo, Brazil

2014 ◽  
Vol 23 (3) ◽  
pp. 348-354 ◽  
Author(s):  
Maurício Orlando Wilmsen ◽  
Bruna Fernanda Silva ◽  
César Cristiano Bassetto ◽  
Alessandro Francisco Talamini do Amarante
Keyword(s):  
Haemonchus Contortus ◽  
Natural Infection ◽  
Gastrointestinal Nematode ◽  
Gastrointestinal Nematodes ◽  
Sao Paulo ◽  
São Paulo ◽  
Mean Intensity ◽  
Oesophagostomum Columbianum ◽  
The Mean ◽  
In Parenthesis

Gastrointestinal nematode infections were evaluated in sheep raised in Botucatu, state of São Paulo, Brazil between April 2008 and March 2011. Every month, two tracer lambs grazing with a flock of sheep were exposed to natural infection with gastrointestinal nematodes for 28 consecutive days. At the end of this period, the lambs were sacrificed for worm counts. Haemonchus contortus presented 100% of prevalence. The seasons exerted no significant influence on the mean intensity of H. contortus, which ranged from 315 worms in November 2010 to 2,5205 worms in January 2011. The prevalence of Trichostrongylus colubriformis was also 100%, with the lowest mean intensity (15 worms) recorded in February 2011 and the highest (9,760 worms) in October 2009. In the case of T. colubriformis, a significant correlation coefficient was found between worm counts vs. rainfall (r = −0.32; P <0.05). Three other nematodes species were found in tracer lambs, albeit in small numbers. Their prevalence and mean intensity (in parenthesis) were as follows: Oesophagostomum columbianum 28% (25.2), Cooperia curticei 7% (4.5) and Trichuris spp. 2% (1). In conclusion, the environmental conditions of the area proved to be highly favorable for the year-round transmission of H. contortus and T. colubriformis.

Sign in / Sign up

Export Citation Format

Share Document