Quantification of human tissue transglutaminase by a luminescence sandwich enzyme-linked immunosorbent assay

2011 ◽  
Vol 419 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Johannes Wolf ◽  
Ingolf Lachmann ◽  
Uta Wagner ◽  
Awad A. Osman ◽  
Thomas Mothes
Keyword(s):  
Immunosorbent Assay ◽  
Human Tissue ◽  
Tissue Transglutaminase ◽  
Enzyme Linked Immunosorbent Assay

Assay of Human Tissue-Type Plasminogen Activator (t-PA) with an Enzyme-Linked Immunosorbent Assay (ELISA) Based on Three Murine Monoclonal Antibodies to t-PA

1985 ◽  
Vol 54 (03) ◽  
pp. 684-687 ◽  
Author(s):  
Paul Holvoet ◽  
Hugo Cleemput ◽  
Désiré Collen
Keyword(s):  
Monoclonal Antibodies ◽  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Human Tissue ◽  
Biological Fluids ◽  
Human Subjects ◽  
Tissue Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Type Plasminogen Activator ◽  
Type Plasminogen Activator

SummaryAn enzyme-linked immunosorbent assay (ELISA) for the measurement of human tissue-type plasminogen activator (t-PA) was developed. Microtiter plates were coated with a mixture of two monoclonal antibodies and bound t-PA was quantitated with a third monoclonal antibody linked to peroxidase. The lower limit of sensitivity of the assay was 0.2 ng of t-PA per ml. The concentration of antigen in citrated plasma of human subjects was found to be 3.4 ± 0.8 ng/ml. The assay had a good reproducibility with values of 3.8, 6.5 and 4.9 percent respectively for the intra-, inter-assay and inter-dilution variation coefficients. The results of the ELISA assay on plasma samples from patients during thrombolytic therapy with t-PA correlated very well, over a wide concentration range, with those obtained with a previously described two-site immuno-radiometric assay (r = 0.96). This ELISA with monoclonal antibodies constitutes a stable and reproducible set of reagents for the measurement of t-PA antigen in biological fluids, avoiding the disadvantages of the use of radioisotopes and of polyclonal antibodies.

scholarly journals Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease

1998 ◽  
Vol 114 ◽  
pp. A420 ◽  
Author(s):  
S. Sulkanen ◽  
T. Halttunen ◽  
I. Korponay-Szabo ◽  
K. Laurila ◽  
P. Collin ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Tissue Transglutaminase ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease

1998 ◽  
Vol 115 (6) ◽  
pp. 1322-1328 ◽  
Author(s):  
Satu Sulkanen ◽  
Tuula Halttunen ◽  
Kaija Laurila ◽  
Kaija-Leena Kolho ◽  
Ilma R. Korponay-Szabó ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Tissue Transglutaminase ◽  
Enzyme Linked Immunosorbent Assay

Enzyme-linked immunosorbent assay for the detection of total cathepsin H in human tissue cytosols and sera

1997 ◽  
Vol 201 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Ana Schweiger ◽  
Borut Štabuc ◽  
Tatjana Popović ◽  
Vito Turk ◽  
Janko Kos
Keyword(s):  
Immunosorbent Assay ◽  
Human Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cathepsin H
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