Mini Review: Impedance Measurement in Neuroscience and Its Prospective Application in the Field of Surgical Neurooncology

Impedance measurement of human tissue can be performed either in vivo or ex vivo. The majority of the in-vivo approaches are non-invasive, and few are invasive. To date, there is no gold standard for impedance measurement of intracranial tissue. In addition, most of the techniques addressing this topic are still experimental and have not found their way into clinical practice. This review covers available impedance measurement approaches in the neuroscience in general and specifically addresses recent advances made in the application of impedance measurement in the field of surgical neurooncology. It will provide an understandable picture on impedance measurement and give an overview of limitations that currently hinders clinical application and require future technical and conceptual solutions.

Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH

The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The positive-sense single-stranded RNA virus contains a single linear RNA segment that serves as a template for transcription and replication, leading to the synthesis of positive and negative-stranded viral RNA (vRNA) in infected cells. Tools to visualize vRNA directly in infected cells are critical to analyze the viral replication cycle, screen for therapeutic molecules, or study infections in human tissue. Here, we report the design, validation, and initial application of FISH probes to visualize positive or negative RNA of SARS-CoV-2 (CoronaFISH). We demonstrate sensitive visualization of vRNA in African green monkey and several human cell lines, in patient samples and human tissue. We further demonstrate the adaptation of CoronaFISH probes to electron microscopy. We provide all required oligonucleotide sequences, source code to design the probes, and a detailed protocol. We hope that CoronaFISH will complement existing techniques for research on SARS-CoV-2 biology and COVID-19 pathophysiology, drug screening, and diagnostics.

Miniaturized Dual-Band Dual-Mode Microstrip Patch Antenna with Defected Ground Structure for Wearable Applications

This work presents the performance of a miniaturized dual-band dual-mode microstrip patch antenna with Defected Ground Structure (DGS) at 2.45 GHz and 5.8 GHz on the stacked substrate configuration in the order of FR-4 – PDMS- FR-4. The antenna offers a promising solution for wearable applications in the ISM bands. The first substrate is a flexible Flame Retardant 4 (FR-4) and the other substrate is a highly flexible Polydimethyl Siloxane (PDMS). The size of the antenna was reduced from 50 × 50 mm2 to 30 × 30 mm2, by introducing DGS on the ground plane. A single U-slot on the rectangular radiating patch was introduced to produce the upper resonant frequency of 5.8 GHz while the existing square patch is to generate the lower resonant frequency of 2.45 GHz. The simulations on the dual-band dual-mode microstrip patch antenna shows the reflection coefficient, S11 at 2.45 GHz is -17.848 dB with a bandwidth of 278.8 MHz and -13.779 dB with a bandwidth of 273 MHz at 5.8 GHz. A unidirectional radiation pattern observed in the E-plane shows that the antenna could be applied for off-body communication while an omnidirectional radiation pattern in the H-plane showed that the antenna can be used for on-body communication. Bending investigation were performed for the antenna over a vacuum cylinder with varying diameters of 50 mm, 60 mm, 70 mm, 80 mm, 90 mm, 100 mm and 120 mm in the CST MWS® software. From the graph of reflection coefficients, the performance of the antenna were not affected in bending condition. The SAR simulations showed that the SAR limits obey the guidelines as stipulated by the Federal Communication Commission (FCC) and the International Commission on Non-Ionizing Radiation Protection (ICNIRP) for 1 mW of input power. The 2.45 GHz SAR limit for 1 g of human tissue is 0.09007 W/kg (FCC standard: < 1.6 W/kg) while for 10 g is 0.01867 W/kg (ICNIRP standard: < 2 W/kg). For 5.8 GHz, the SAR limit for 1 g of human tissue is 0.115 W/kg and for 10 g is 0.03517 W/kg. Based on the performance of the antenna in bending condition and the SAR limits, it is safe to conclude that the antenna can be used for wearable applications at 2.45 GHz and 5.8 GHz of the ISM bands.

The electronic tree of life (eTOL): a net of long probes to characterize the human microbiome from RNA-seq data

Background Microbiome analysis generally requires PCR-based or metagenomic shotgun sequencing, sophisticated programs, and large volumes of data. Alternative approaches based on widely available RNA-seq data are constrained because of sequence similarities between the transcriptomes of microbes/viruses and those of the host, compounded by the extreme abundance of host sequences in such libraries. Current approaches are also limited to specific microbial groups. There is a need for alternative methods of microbiome analysis that encompass the entire tree of life. Results We report a method to specifically retrieve non-human sequences in human tissue RNA-seq data. For cellular microbes we used a bioinformatic 'net', based on filtered 64-mer small subunit rRNA sequences across the Tree of Life (the 'electronic tree of life', eTOL), to comprehensively (98%) entrap all non-human rRNA sequences present in the target tissue. Using brain as a model, retrieval of matching reads, re-exclusion of human-related sequences, followed by contig building and species identification, is followed by reconfirmation of the abundance and identity of the corresponding species groups. We provide methods to automate this analysis. A variant approach is necessary for viruses. Again, because of significant matches between viral and human sequences, a 'stripping' approach is essential. In addition, contamination during workup is a potential problem, and we discuss strategies to circumvent this issue. To illustrate the versatility of the method, we report the use of the eTOL methodology to unambiguously identify exogenous microbial and viral sequences in human tissue RNA-seq data across the entire tree of life including Archaea, Bacteria, Chloroplastida, basal Eukaryota, Fungi, and Holozoa/Metazoa, and discuss the technical and bioinformatic challenges involved. Conclusions This generic methodology may find wider application in microbiome analysis including diagnostics.

Law and Legacy in Medical Jurisprudence

Graeme Laurie stepped down from the Chair in Medical Jurisprudence at the University of Edinburgh in 2019. This edited collection pays tribute to his extraordinary contributions to the field. Graeme often spoke about the importance of 'legacy' in academic work and forged a remarkable intellectual legacy of his own, notably through his work on genetic privacy, human tissue and information governance, and the regulatory salience of the concept of liminality. The essays in this volume animate the concept of legacy to analyse the study and practice of medical jurisprudence. In this light, legacy reveals characteristics of both benefit and burden, as both an encumbrance to and facilitator of the development of law, policy and regulation. The contributions reconcile the ideas of legacy and responsiveness and show that both dimensions are critical to achieve and sustain the health of medical jurisprudence itself as a dynamic, interdisciplinary and policy-engaged field of thinking.

Pancreatic Islet RNA Extraction v1

This SOP defines the methods used by the Vanderbilt Diabetes Center Islet and Pancreas Analysis (IPA) Core for RNA extraction of pancreatic islets isolated from mouse or human tissue.

Static Incubation of Pancreatic Islets v1

This SOP defines the methods used by the Vanderbilt Diabetes Center Islet and Pancreas Analysis (IPA) Core for static incubation of pancreatic islets isolated from mouse or human tissue. See also our islet isolation protocol.