A SNP at MicroRNA binding site of epidermal growth factor receptor 3́ untranslated region associated with Yangzhou geese egg production

Animal Gene ◽  
2022 ◽  
Vol 23 ◽  
pp. 200123
Author(s):  
Murtada Alsiddig ◽  
Tarig Badri ◽  
Hind Widaa ◽  
Bojiang Li ◽  
Yu Shigang ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Binding Site ◽  
Untranslated Region ◽  
Egg Production ◽  
Growth Factor Receptor ◽  
Epidermal Growth

scholarly journals Transcriptional activation of the human epidermal growth factor receptor promoter by human p53.

1996 ◽  
Vol 16 (11) ◽  
pp. 6009-6019 ◽  
Author(s):  
J H Ludes-Meyers ◽  
M A Subler ◽  
C V Shivakumar ◽  
R M Munoz ◽  
P Jiang ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Binding Site ◽  
Growth Factor Receptor ◽  
Mutant P53 ◽  
Wild Type ◽  
Response Element ◽  
Epidermal Growth ◽  
Wild Type P53

The human epidermal growth factor receptor (EGFR) promoter is activated by both wild-type and tumor-derived mutant p53. In this communication, we demonstrate that EGFR promoter sequence requirements for transactivation by wild-type and mutant p53 are different. Transient-expression assays with EGFR promoter deletions identified a wild-type human p53 response element, 5'-AGCTAGACGTCCGGGCAGCCCCCGGCG -3', from positions --265 to --239. Electrophoretic mobility shift analysis and DNase I footprinting assays indicated that wild-type p53 binds sequence specifically to the response element. Using circularly permuted DNA fragments containing the p53-binding site, we show that wild-type p53 binding induces DNA bending at this site. We further show that the EGFR promoter is also activated by tumor-derived p53 mutants p53-143A, p53-175H, p53-248W, p53-273H, and p53-281G. However, the transactivation by mutant p53 does not require the wild-type p53-binding site. The minimal EGFR promoter from positions --104 to --20 which does not contain the wild-type p53-binding site is transactivated by the p53 mutants but not by the wild-type protein, showing a difference in the mechanism of transactivation by wild-type and mutant p53. Transactivation of the EGFR promoter by p53 may represent a novel mechanism of cell growth regulation.

Characterization of a monoclonal antibody directed against the epidermal growth factor receptor binding site

1991 ◽  
Vol 34 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Rita Pellegrini ◽  
Filippo Centis ◽  
Stefania Martignone ◽  
Antonio Mastroianni ◽  
Elda Tagliabue ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Binding Site ◽  
Receptor Binding ◽  
Growth Factor Receptor ◽  
Receptor Binding Site ◽  
Epidermal Growth
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