scholarly journals Histamine H4 receptor–RGS fusion proteins expressed in Sf9 insect cells: A sensitive and reliable approach for the functional characterization of histamine H4 receptor ligands

2009 ◽  
Vol 78 (6) ◽  
pp. 607-616 ◽  
Author(s):  
Erich H. Schneider ◽  
Roland Seifert
Keyword(s):  
Fusion Proteins ◽  
Insect Cells ◽  
Functional Characterization ◽  
Receptor Ligands ◽  
Histamine H4 Receptor ◽  
Sf9 Insect Cells ◽  
H4 Receptor

Functional characterization of histamine H4 receptor on human mast cells

2014 ◽  
Vol 62 (1) ◽  
pp. 19-28 ◽  
Author(s):  
E. Angel Jemima ◽  
A. Prema ◽  
E. Berla Thangam
Keyword(s):  
Mast Cells ◽  
Functional Characterization ◽  
Histamine H4 Receptor ◽  
Human Mast Cells ◽  
H4 Receptor

scholarly journals Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Xinsheng Liu ◽  
Jianliang Lv ◽  
Yuzhen Fang ◽  
Peng Zhou ◽  
Yanzhen Lu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Virus ◽  
Fusion Proteins ◽  
Insect Cells ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Sf9 Insect Cells ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Improving vaccine immunogenicity by targeting antigens to dendritic cells has recently emerged as a new design strategy in vaccine development. In this study, the VP1 gene of foot-and-mouth disease virus (FMDV) serotype A was fused with the gene encoding human immunodeficiency virus (HIV) membrane glycoprotein gp120 or C2-V3 domain of hepatitis C virus (HCV) envelope glycoprotein E2, both of which are DC-SIGN-binding glycoproteins. After codon optimization, the VP1 protein and the two recombinant VP1-gp120 and VP1-E2 fusion proteins were expressed in Sf9 insect cells using the insect cell-baculovirus expression system. Western blotting showed that the VP1 protein and two recombinant VP1-gp120 and VP1-E2 fusion proteins were correctly expressed in the Sf9 insect cells and had good reactogenicity. Guinea pigs were then immunized with the purified proteins, and the resulting humoral and cellular immune responses were analyzed. The VP1-gp120 and VP1-E2 fusion proteins induced significantly higher specific anti-FMDV antibody levels than the VP1 protein and stronger cell-mediated immune responses. This study provides a new perspective for the development of novel FMDV subunit vaccines.

Functional Characterization of GABAA Receptor Ligands In Vitro

2004 ◽  
pp. 85-94
Author(s):  
Bjarke Ebert ◽  
Sally Anne Thompson ◽  
Signe Í. Stórustovu ◽  
Keith A. Wafford
Keyword(s):  
Gabaa Receptor ◽  
Functional Characterization ◽  
Receptor Ligands

Expression, purification, and functional characterization of adenovirus 5 and 12 E1A proteins produced in insect cells

Virology ◽  
1992 ◽  
Vol 190 (2) ◽  
pp. 733-745 ◽  
Author(s):  
Daniel S. Peeper ◽  
Alt Zantema ◽  
Steven F. Dowdy ◽  
Alex J. van der Eb
Keyword(s):  
Insect Cells ◽  
Functional Characterization ◽  
Adenovirus 5
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