The effect of functionalized self-assembling peptide scaffolds on human aortic endothelial cell function

Biomaterials ◽  
2005 ◽  
Vol 26 (16) ◽  
pp. 3341-3351 ◽  
Author(s):  
Elsa Genové ◽  
Colette Shen ◽  
Shuguang Zhang ◽  
Carlos E. Semino
Keyword(s):  
Endothelial Cell ◽  
Cell Function ◽  
Human Aortic Endothelial Cell ◽  
Endothelial Cell Function ◽  
Aortic Endothelial Cell ◽  
Self Assembling ◽  
Peptide Scaffolds ◽  
Aortic Endothelial

scholarly journals Role of lipid phosphate phosphatase 3 in human aortic endothelial cell function

2016 ◽  
Vol 112 (3) ◽  
pp. 702-713 ◽  
Author(s):  
Zahia Touat-Hamici ◽  
Henri Weidmann ◽  
Yuna Blum ◽  
Carole Proust ◽  
Hervé Durand ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Function ◽  
Human Aortic Endothelial Cell ◽  
Endothelial Cell Function ◽  
Aortic Endothelial Cell ◽  
Lipid Phosphate ◽  
Aortic Endothelial

scholarly journals Polyploidy impairs human aortic endothelial cell function and is prevented by nicotinamide phosphoribosyltransferase

2010 ◽  
Vol 298 (1) ◽  
pp. C66-C74 ◽  
Author(s):  
Nica M. Borradaile ◽  
J. Geoffrey Pickering
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Replicative Senescence ◽  
Cell Function ◽  
Human Aortic Endothelial Cell ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Endothelial Cell Function ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Polyploid endothelial cells are found in aged and atherosclerotic arteries. However, whether increased chromosome content has an impact on endothelial cell function is unknown. We show here that human aortic endothelial cells become tetraploid as they approach replicative senescence. Furthermore, accumulation of tetraploid endothelial cells was accelerated during growth in high glucose. Interestingly, induction of polyploidy was completely prevented by modest overexpression of the NAD+ regenerating enzyme, nicotinamide phosphoribosyltransferase (Nampt). To determine the impact of polyploidy on endothelial cell function, independent of replicative senescence, we induced tetraploidy using the spindle poison, nocodazole. Global gene expression analyses of tetraploid endothelial cells revealed a dysfunctional phenotype characterized by a cell cycle arrest profile (decreased CCNE2/A2, RBL1, BUB1B; increased CDKN1A) and increased expression of genes involved in inflammation ( IL32, TNFRSF21/10C, PTGS1) and extracellular matrix remodeling ( COL5A1, FN1, MMP10/14). The protection from polyploidy conferred by Nampt was not associated with enhanced poly(ADP-ribose) polymerase-1 or sirtuin (SIRT) 2 activity, but with increased SIRT1 activity, which reduced cellular reactive oxygen species and the associated oxidative stress stimulus for the induction of polyploidy. We conclude that human aortic endothelial cells are prone to chromosome duplication that, in and of itself, can induce characteristics of endothelial dysfunction. Moreover, the emergence of polyploid endothelial cells during replicative aging and glucose overload can be prevented by optimizing the Nampt-SIRT1 axis.

Human aortic endothelial cell aminopeptidase N

1996 ◽  
Vol 32 (1-3) ◽  
pp. 153-156 ◽  
Author(s):  
Andreas Papapetropoulos ◽  
James W Ryan ◽  
Alexander Antonov ◽  
Renu Virmani ◽  
Frank D Kolodgie ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Aminopeptidase N ◽  
Human Aortic Endothelial Cell ◽  
Aortic Endothelial Cell ◽  
Aortic Endothelial

scholarly journals Effects of macro- versus nanoporous silicon substrates on human aortic endothelial cell behavior

2014 ◽  
Vol 9 (1) ◽  
pp. 421 ◽  
Author(s):  
Pilar Formentín ◽  
María Alba ◽  
Úrsula Catalán ◽  
Sara Fernández-Castillejo ◽  
Josep Pallarès ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Behavior ◽  
Silicon Substrates ◽  
Human Aortic Endothelial Cell ◽  
Nanoporous Silicon ◽  
Aortic Endothelial Cell ◽  
Aortic Endothelial

scholarly journals Spatial regulation of shear stress in cultured human aortic endothelial cell functions.

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Jean Tsou ◽  
Harold J Ting ◽  
Ulrich Y Schaff ◽  
Michael F Insana ◽  
Scott I Simon
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Human Aortic Endothelial Cell ◽  
Aortic Endothelial Cell ◽  
Spatial Regulation ◽  
Cell Functions ◽  
Aortic Endothelial

scholarly journals Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jeny Shklover ◽  
James McMasters ◽  
Alba Alfonso-Garcia ◽  
Manuela Lopera Higuita ◽  
Alyssa Panitch ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cell ◽  
Cell Growth ◽  
Basement Membrane ◽  
Inflammatory Marker ◽  
Cell Phenotype ◽  
Human Aortic Endothelial Cell ◽  
Aortic Endothelial Cell ◽  
And Function ◽  
Aortic Endothelial

Abstract Xenogeneic biomaterials contain biologically relevant extracellular matrix (ECM) composition and organization, making them potentially ideal surgical grafts and tissue engineering scaffolds. Defining the effect of ECM niche (e.g., basement membrane vs. non-basement membrane) on repopulating cell phenotype and function has important implications for use of xenogeneic biomaterials, particularly in vascular applications. We aim to understand how serous (i.e., basement membrane) versus fibrous (i.e., non-basement membrane) ECM niche of antigen-removed bovine pericardium (AR-BP) scaffolds influence human aortic endothelial cell (hAEC) adhesion, growth, phenotype, inflammatory response and laminin production. At low and moderate seeding densities hAEC proliferation was significantly increased on the serous side. Similarly, ECM niche modulated cellular morphology, with serous side seeding resulting in a more rounded aspect ratio and intact endothelial layer formation. At moderate seeding densities, hAEC production of human laminin was enhanced following serous seeding. Finally, inflammatory marker and pro-inflammatory cytokine expression decreased following long-term cell growth regardless of seeding side. This work demonstrates that at low and moderate seeding densities AR-BP sidedness significantly impacts endothelial cell growth, morphology, human laminin production, and inflammatory state. These findings suggest that ECM niche has a role in modulating response of repopulating recipient cells toward AR-BP scaffolds for vascular applications.

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