Towards whole-body imaging at the single cell level using ultra-sensitive stem cell labeling with oligo-arginine modified upconversion nanoparticles

Biomaterials ◽  
2012 ◽  
Vol 33 (19) ◽  
pp. 4872-4881 ◽  
Author(s):  
Chao Wang ◽  
Liang Cheng ◽  
Huan Xu ◽  
Zhuang Liu
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Cell Labeling ◽  
Whole Body ◽  
Upconversion Nanoparticles ◽  
Whole Body Imaging ◽  
Single Cell Level ◽  
Cell Level ◽  
Body Imaging

scholarly journals Dynamic Imaging of Marrow-Resident Granulocytes Interacting with Human Mesenchymal Stem Cells upon Systemic Lipopolysaccharide Challenge

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Jay T. Myers ◽  
Deborah S. Barkauskas ◽  
Alex Y. Huang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Human Mesenchymal Stem Cells ◽  
Dynamic Imaging ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Single Cell Level ◽  
Cell Level

Human mesenchymal stem cells (hMSCs) have gained intense research interest due to their immune-modulatory, tissue differentiating, and homing properties to sites of inflammation. Despite evidence demonstrating the biodistribution of infused hMSCs in target organs using static fluorescence imaging or whole-body imaging techniques, surprisingly little is known about how hMSCs behave dynamically within host tissues on a single-cell levelin vivo. Here, we infused fluorescently labeled clinical-grade hMSCs into immune-competent mice in which neutrophils and monocytes express a second fluorescent marker under the lysozyme M (LysM) promoter. Using intravital two-photon microscopy (TPM), we were able for the first time to capture dynamic interactions between hMSCs and LysM+granulocytes in the calvarium bone marrow of recipient mice during systemic LPS challenge in real time. Interestingly, many of the infused hMSCs remained intact despite repeated cellular contacts with host neutrophils. However, we were able to observe the destruction and subsequent phagocytosis of some hMSCs by surrounding granulocytes. Thus, our imaging platform provides opportunities to gain insight into the biology and therapeutic mechanisms of hMSCsin vivoat a single-cell level within live hosts.

scholarly journals Cellular Barcoding Links B-1a B Cell Potential to a Fetal Hematopoietic Stem Cell State at the Single-Cell Level

Immunity ◽  
2016 ◽  
Vol 45 (2) ◽  
pp. 346-357 ◽  
Author(s):  
Trine A. Kristiansen ◽  
Elin Jaensson Gyllenbäck ◽  
Alya Zriwil ◽  
Tomas Björklund ◽  
Jeremy A. Daniel ◽  
...  
Keyword(s):  
Stem Cell ◽  
B Cell ◽  
Single Cell ◽  
Hematopoietic Stem Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Cell Potential ◽  
Hematopoietic Stem ◽  
Stem Cell State ◽  
Cellular Barcoding

33 Single-Cell Level Analysis Reveals Heterogeneous Expression of Stem-Cell Specific Genes in Human Small Intestinal Organoids

2016 ◽  
Vol 150 (4) ◽  
pp. S11
Author(s):  
Kohei Suzuki ◽  
Satoru Fujii ◽  
Ami Kawamoto ◽  
Fumiaki Ishibashi ◽  
Toru Nakata ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Intestinal Organoids ◽  
Heterogeneous Expression ◽  
Small Intestinal ◽  
Level Analysis

scholarly journals Single-cell transcriptome provides novel insights into antler stem cells, a cell type capable of mammalian organ regeneration

2018 ◽  
Author(s):  
Hengxing Ba ◽  
Datao Wang ◽  
Weiyao Wu ◽  
Hongmei Sun ◽  
Chunyi Li
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Single Cell ◽  
Large Cell ◽  
Stem Cell Markers ◽  
Single Cell Level ◽  
Cell Type ◽  
Cell Level ◽  
Homogeneous Population ◽  
Organ Regeneration

AbstractAntler regeneration, a stem cell-based epimorphic process, has potential as a valuable model for regenerative medicine. A pool of antler stem cells (ASCs) for antler development is located in the antlerogenic periosteum (AP). However, whether this ASC pool is homogenous or heterogeneous has not been fully evaluated. In this study, we produced a comprehensive transcriptome dataset at the single-cell level for the ASCs based on the 10x Genomics platform (scRNA-seq). A total of 4,565 ASCs were sequenced and classified into a large cell cluster, indicating that the ASCs resident in the AP are likely to be a homogeneous population. The scRNA-seq data revealed that tumor-related genes were highly expressed in these homogeneous ASCs: i.e. TIMP1, TMSB10, LGALS1, FTH1, VIM, LOC110126017 and S100A4. Results of screening for stem cell markers suggest that the ASCs may be considered as a special type of stem cell between embryonic (CD9) and adult (CD29, CD90, NPM1 and VIM) stem cells. Our results provide the first comprehensive transcriptome analysis at the single-cell level for the ASCs, and identified only one major cell type resident in the AP and some key stem cell genes, which may hold the key to why antlers, the unique mammalian organ, can fully regenerate once lost.

Co-detection of ALDH1A1, ABCG2, ALCAM and CD133 in three A549 subpopulations at the single cell level by one-step digital RT-PCR

2018 ◽  
Vol 10 (6) ◽  
pp. 364-369 ◽  
Author(s):  
Yanan Xu ◽  
Jiumei Hu ◽  
Qiangyuan Zhu ◽  
Qi Song ◽  
Ying Mu
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Analysis ◽  
Rt Pcr ◽  
Cell Level ◽  
One Step ◽  
Single Cancer ◽  
Co Detection

Single cancer stem cell analysis of four biomarker genes by microfluidic one-step digital RT-PCR.

scholarly journals The stem cell niche: tissue physiology at a single cell level

2012 ◽  
Vol 122 (9) ◽  
pp. 3029-3034 ◽  
Author(s):  
Jonathan Hoggatt ◽  
David T. Scadden
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Stem Cell Niche ◽  
Single Cell Level ◽  
Cell Level ◽  
Cell Niche

scholarly journals In Vivo Magnetic Resonance Tracking of Magnetically Labeled Cells after Transplantation

2002 ◽  
Vol 22 (8) ◽  
pp. 899-907 ◽  
Author(s):  
Jeff W. M. Bulte ◽  
Ian D. Duncan ◽  
Joseph A. Frank
Keyword(s):  
Stem Cell ◽  
Magnetic Resonance ◽  
Mr Imaging ◽  
Superparamagnetic Iron Oxide ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Body Imaging ◽  
Stem And Progenitor Cells ◽  
The Central Nervous System

During the last few years, the therapeutic use of stem and progenitor cells as a substitute for malfunctioning endogenous cell populations has received considerable attention. Unlike their current use in animal models, the introduction of therapeutic cells in patients will require techniques that can monitor their tissue biodistribution noninvasively. Among the different imaging modalities, magnetic resonance (MR) imaging offers both near-cellular (i.e., 25- to 50-μ) resolution and whole-body imaging capability. In order to be visualized, cells must be labeled with an intracellular tracer molecule that can be detected by MR imaging. Methods have now been developed that make it possible to incorporate sufficient amounts of superparamagnetic iron oxide into cells, enabling their detection in vivo using MR imaging. This is illustrated for (neural stem cell—derived) magnetically labeled oligodendroglial progenitors, transplanted in the central nervous system of dysmyelinated rats. Cells can be followed in vivo for at least 6 weeks after transplantation, with a good histopathologic correlation including the formation of myelin. Now that MR tracking of magnetically labeled cells appears feasible, it is anticipated that this technique may ultimately become an important tool for monitoring the efficacy of clinical (stem) cell transplantation protocols.

scholarly journals Optical tweezers system for live stem cell organization at the single-cell level

2018 ◽  
Vol 9 (2) ◽  
pp. 771 ◽  
Author(s):  
Peifeng Jing ◽  
Yannan Liu ◽  
Ethan G. Keeler ◽  
Nelly M. Cruz ◽  
Benjamin S. Freedman ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Optical Tweezers ◽  
Single Cell Level ◽  
Cell Level ◽  
Cell Organization ◽  
Live Stem Cell

scholarly journals Crucial stem cell experiments? Stem cells, uncertainty, and single-cell experiments

2015 ◽  
Vol 30 (2) ◽  
pp. 183
Author(s):  
Melinda Fagan
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Single Cell ◽  
Single Cells ◽  
Single Cell Level ◽  
Epistemic Circularity ◽  
Cell Level ◽  
Differentiation Potential ◽  
Self Renewal

I have previously argued that stem cell experiments cannot in principle demonstrate that a single cell is a stem cell ([reference omitted for anonymous review]).  Laplane and others dispute this claim, citing experiments that identify stem cells at the single-cell level.  This paper rebuts the counterexample, arguing that these alleged ‘crucial stem cell experiments’ do not measure self-renewal for a single cell, do not establish a single cell’s differentiation potential, and, if interpreted as providing results about single cells, fall into epistemic circularity.  I then examine the source of the dispute, noting differences in philosophical and experimental perspectives.

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