Enhancement of lipid productivity by adopting multi-stage continuous cultivation strategy in Nannochloropsis gaditana

2017 ◽  
Vol 229 ◽  
pp. 20-25 ◽  
Author(s):  
Min-Gyu Sung ◽  
Bongsoo Lee ◽  
Chul Woong Kim ◽  
Kibok Nam ◽  
Yong Keun Chang
Keyword(s):  
Continuous Cultivation ◽  
Lipid Productivity ◽  
Nannochloropsis Gaditana ◽  
Multi Stage ◽  
Cultivation Strategy

Effect of monochromatic illumination on lipid accumulation of Nannochloropsis gaditana under continuous cultivation

2014 ◽  
Vol 159 ◽  
pp. 30-35 ◽  
Author(s):  
Chul Woong Kim ◽  
Min-Gyu Sung ◽  
Kibok Nam ◽  
Myounghoon Moon ◽  
Jong-Hee Kwon ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Continuous Cultivation ◽  
Nannochloropsis Gaditana ◽  
Monochromatic Illumination

scholarly journals Operation Regimes: A Comparison Based on Nannochloropsis oceanica Biomass and Lipid Productivity

Energies ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1542
Author(s):  
Inês Guerra ◽  
Hugo Pereira ◽  
Margarida Costa ◽  
Joana T. Silva ◽  
Tamára Santos ◽  
...  
Keyword(s):  
Large Scale ◽  
Continuous Cultivation ◽  
Fold Increase ◽  
Continuous Operation ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Nannochloropsis Oceanica ◽  
Operation Regime ◽  
Significant Research ◽  
Tubular Photobioreactors

Microalgae are currently considered to be a promising feedstock for biodiesel production. However, significant research efforts are crucial to improve the current biomass and lipid productivities under real outdoor production conditions. In this context, batch, continuous and semi-continuous operation regimes were compared during the Spring/Summer seasons in 2.6 m3 tubular photobioreactors to select the most suitable one for the production of the oleaginous microalga Nannochloropsis oceanica. Results obtained revealed that N. oceanica grown using the semi-continuous and continuous operation regimes enabled a 1.5-fold increase in biomass volumetric productivity compared to that cultivated in batch. The lipid productivity was 1.7-fold higher under semi-continuous cultivation than that under a batch operation regime. On the other hand, the semi-continuous and continuous operation regimes spent nearly the double amount of water compared to that of the batch regime. Interestingly, the biochemical profile of produced biomass using the different operation regimes was not affected regarding the contents of proteins, lipids and fatty acids. Overall, these results show that the semi-continuous operation regime is more suitable for the outdoor production of N. oceanica, significantly improving the biomass and lipid productivities at large-scale, which is a crucial factor for biodiesel production.

scholarly journals Isolation of Microalgae from Mediterranean Seawater and Production of Lipids in the Cultivated Species

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1601
Author(s):  
Imane Haoujar ◽  
Francesco Cacciola ◽  
Manuel Manchado ◽  
Jamal Abrini ◽  
Mohammed Haoujar ◽  
...  
Keyword(s):  
High Performance ◽  
Climatic Conditions ◽  
Lipid Productivity ◽  
Morphological Identification ◽  
Important Research ◽  
Research Topics ◽  
Isolation And Identification ◽  
Nannochloropsis Gaditana ◽  
High Lipid ◽  
Cultivated Species

Isolation and identification of novel microalgae strains with high lipid productivity is one of the most important research topics to have emerged recently. However, practical production processes will probably require the use of local strains adapted to commanding climatic conditions. The present manuscript describes the isolation of 96 microalgae strains from seawater located in Bay M’diq, Morocco. Four strains were identified using the 18S rDNA and morphological identification through microscopic examination. The biomass and lipid productivity were compared and showed good results for Nannochloris sp. (15.93 mg/L/day). The lipid content in the four species, namely Nannochloropsis gaditana, Nannochloris sp., Phaedactylum tricornutum and Tetraselmis suecica, was carried out by high performance liquid chromatography coupled to mass spectrometry (HPLC-MS ) highlighting the identification of up to 77 compounds.

Effect of nitrogen addition on lipid productivity of nitrogen starved Nannochloropsis gaditana

2018 ◽  
Vol 33 ◽  
pp. 125-132 ◽  
Author(s):  
Jorijn H. Janssen ◽  
Jens Kastenhofer ◽  
Jacob A. de Hoop ◽  
Packo P. Lamers ◽  
René H. Wijffels ◽  
...  
Keyword(s):  
Nitrogen Addition ◽  
Lipid Productivity ◽  
Nannochloropsis Gaditana

scholarly journals Microalgae as a Source of Functional PUFAs: A Green Low-cost Pathway via Enzymatic Hydrolysis

2019 ◽  
pp. 1-13
Author(s):  
Eduardo Henrique Bredda ◽  
Messias Borges Silva ◽  
Heizir Ferreira De Castro ◽  
Ana Paula Teixeira Da Silva ◽  
Patrícia Caroline Molgero da Rós
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Burkholderia Cepacia ◽  
Rhizopus Oryzae ◽  
Candida Rugosa ◽  
Lipid Productivity ◽  
Microbial Oil ◽  
Alternative Source ◽  
Nannochloropsis Gaditana ◽  
Microalgal Oil

Microalgal oil is an alternative source of polyunsaturated fatty acids (PUFAs) that can replace conventional ones such as vegetable and fish oils. In this work, Nannochloropsis gaditana oil was investigated as a source of high-value polyunsaturated fatty acids. The cultivation conditions were optimized using a 22 full factorial design to simultaneously assess the influence of NaNO3 (0.075 to 0.225 mg.L-1) and CH3COONa (2 to 6 g.L-1) concentrations on the lipid productivity. All cultivations were done in 4 L tank photo bioreactors for 7 days, yielding 21.87 mg.L-1.day-1 maximum lipid productivity when using 225 mg.L-1 of nitrate and 6 g.L-1 of acetate. The obtained microbial oil was further characterized revealing palmitic (22.1%), oleic (22.3%), and linoleic (17.0%) as the main fatty acids. The resulting microbial oil was employed in the hydrolysis reactions and different sources of lipases as biocatalysts. The highest performance was achieved by the lipases Burkholderia cepacia (86%), Candida rugosa (84%), and Rhizopus oryzae (82%).

scholarly journals Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stefan Kittler ◽  
Christoph Slouka ◽  
Andreas Pell ◽  
Roman Lamplot ◽  
Mihail Besleaga ◽  
...  
Keyword(s):  
Protein Expression ◽  
Recombinant Protein Expression ◽  
Continuous Cultivation ◽  
Continuous Processes ◽  
E Coli ◽  
Industrial Sectors ◽  
Golden Standard ◽  
Cultivation Strategy ◽  
Upstream Processing

AbstractIn many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant protein expression can be spatially separated. This cultivation mode was found to facilitate stable protein expression using microbial hosts, however mechanistic knowledge of this cultivation strategy is scarce. In this contribution we present a method workflow to reduce workload and accelerate the establishment of stable continuous processes with E. coli BL21(DE3) exclusively based on bioengineering methods.

scholarly journals Nannochloropsis gaditana and Dunaliella salina as Feedstock for Biodiesel Production: Lipid Production and Biofuel Quality

2019 ◽  
pp. 1-10
Author(s):  
Eduardo Henrique Bredda ◽  
Patrícia Caroline Molgero Da Rós ◽  
Guilherme Arantes Pedro ◽  
Heizir Ferreira de Castro ◽  
Messias Borges Silva
Keyword(s):  
Fatty Acids ◽  
Sodium Bicarbonate ◽  
Sodium Acetate ◽  
Dunaliella Salina ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Nannochloropsis Gaditana ◽  
Biodiesel Properties ◽  
Wide Range ◽  
Microalgal Lipids

Introduction: Microalgal lipids have a wide range of applications, from biodiesel manufacture in the energy industry to the production of polyunsaturated fatty acids for the pharmaceutical industry. Microalgal lipid productivity and quality, however, vary greatly depending on cultivation parameters. Aims: In this study, we investigated the potential of two marine microalgae, Nannochloropsis gaditana and Dunaliella salina, to be used as feedstock for biodiesel production. Methodology: A Taguchi L4 orthogonal array design was applied to understand the effects of sodium acetate (0 or 2 g L−1), sodium bicarbonate (0 or 2 g L−1), and sodium nitrate (25 or 75 mg L−1) concentrations on biomass and lipid productivities. Fatty acid methyl ester (FAME) profiles of microalgal lipids obtained under the best conditions were determined, and FAME results were used to predict biodiesel properties. Results: Both carbon sources (sodium acetate and sodium bicarbonate) improved biomass productivity. Lipid productivity was enhanced only by sodium acetate. The highest lipid productivities obtained were 10.25 ± 1.02 and 12.12 ± 0.28 mg L−1 day−1 for N. gaditana and D. salina, respectively. Palmitic (C16:0), stearic (C18:1), oleic (C18:1), linoleic (C18:2), lauric (C12:0), and myristic (C14:0) acids were the major components of D. salina oil. The major fatty acids in N. gaditana oil were C16:0, C18:0, and C18:1. Conclusion: The great differences in FAME profiles resulted in different biodiesel properties. Biodiesel from N. gaditana oil was predicted to have a higher cetane number (73.20) than that derived from D. salina oil (59.59). D. salina oil biodiesel, however, was predicted to have better properties than N. gaditana oil biodiesel, including lower cloud point (0.46°C) and cold filter plugging point (−7.27°C).

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