An integrated process for mixed culture production of 3-hydroxyhexanoate-rich polyhydroxyalkanoates from fruit waste

2021 ◽  
pp. 131908
Author(s):  
Fernando Silva ◽  
Mariana Matos ◽  
Bruno Pereira ◽  
Cláudia Ralo ◽  
Daniela Pequito ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Integrated Process ◽  
Fruit Waste ◽  
Culture Production

Impact of Transfaunation of Rumen Ciliate Cultures on Physical Examination, Selected Serum Parameters and Milk Profile in Defaunated Lactating Dairy Goats

2020 ◽  
Keyword(s):  
Physical Examination ◽  
Mixed Culture ◽  
Pure Culture ◽  
Milk Fat ◽  
Mixed Cultures ◽  
Dairy Goats ◽  
Serum Parameters ◽  
Native Breed ◽  
Culture Duration ◽  
Inorganic Phosphorous

Rumen ciliates still have mysterious secrets and influences in ruminants. This study investigated the effect of transfaunation of pure and mixed cultures of rumen ciliates on physical clinical examination, selected serum parameters and milk profile in defaunated lactating dairy goats. A number of 8 Baladi native breed goats were randomly classified into two groups each one containing 4 goats. Pure culture group was transfaunated with 6 ml of pure culture of Holotricha spp., while mixed culture group was transfaunated with 6 ml of mixed culture of 81.85% Holotricha and 18.15% Ophryoscolex spp. once weekly for three consecutive weeks, after defaunation of both groups using 30 ml of 8% SLS for two consecutive days. Serum and milk samples were collected weekly for three successive weeks to study effect of type of ciliate culture, duration of transfaunation and their interaction. Results revealed that transfaunation of pure and mixed cultures of rumen ciliates had no effect on physical examination with minimal non-significant improvement of calcium, inorganic phosphorous, total protein and globulin in serum of defaunated goats. Transfaunation of pure or mixed cultures of rumen ciliates within three weeks could not improve significantly decreased milk fat % of defaunated goats without any effect on other measured milk profile parameters. It is concluded that further investigations on transfaunation without prior defaunation should be performed using different pure and mixed cultures of rumen ciliates for therapeutic and productive purposes.

Effect of sulfate on anaerobic processes fed with dual substrates

1995 ◽  
Vol 31 (9) ◽  
pp. 101-107 ◽  
Author(s):  
Chongchin Polprasert ◽  
Charles N. Haas
Keyword(s):  
Acetic Acid ◽  
Sulfate Reduction ◽  
Mixed Culture ◽  
Biogas Production ◽  
Cod Removal ◽  
Methane Formation ◽  
Batch Mode ◽  
Sulfate Reducing ◽  
Anaerobic Reactors ◽  
Optimal Feed

Anaerobic reactors were operated in a semi-batch mode and fed with the dual substrates glucose (G) plus acetic acid (Ac) as primary organic sources to study the effect of sulfate on COD oxidation. With glucose, COD removal by methane formation was seriously inhibited, resulting in COD accumulation in the reactor. Although acetic acid can be consumed by some sulfate-reducing species, it was not a major substrate for sulfate reduction, but was largely responsible for methane formation in the anaerobic mixed culture used in this study. With dual substrates, extreme inhibition of methanogenesis did not occur as did with glucose alone. Instead, methanogens were found to work in harmony with acid formers as well as sulfate reducers to oxidise COD. Interestingly, from 12-hour monitoring, increased G/Ac COD ratios decreased COD removal rates as well as biogas production, but resulted in higher sulfate reduction. This suggests that there should be an optimal feed G/Ac COD ratio, for which removal of both organics could be maximised.

Bioleaching of complex polymetallic sulfide ores by mixed culture

2014 ◽  
Vol 21 (7) ◽  
pp. 2633-2637 ◽  
Author(s):  
Jun Wang ◽  
Hong-bo Zhao ◽  
Wen-qing Qin ◽  
Guan-zhou Qiu
Keyword(s):  
Mixed Culture ◽  
Sulfide Ores
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