New oxidant used for the post-column derivatization determination of Malachite Green and Leucomalachite Green residues in cultured aquatic products by high-performance liquid chromatography

2008 ◽  
Vol 1203 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Chaoyang Long ◽  
Zhibin Mai ◽  
Binghui Zhu ◽  
Xiaoyong Zou ◽  
Yanhong Gao ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Malachite Green ◽  
High Performance ◽  
Aquatic Products ◽  
Leucomalachite Green

Determination of Residues of Malachite Green and Leucomalachite Green in Chinese Soft-Shelled Turtle (Pelodiscus sinensis) Muscle by Ultra-High Performance Liquid Chromatography-Mass Spectrometry

2013 ◽  
Vol 781-784 ◽  
pp. 1479-1484
Author(s):  
Shao Dong Zeng ◽  
Chun Liang Yang ◽  
Ming Yue Wang ◽  
Yu Bing Cha ◽  
Ling Lin
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Malachite Green ◽  
High Performance ◽  
Pelodiscus Sinensis ◽  
Neutral Alumina ◽  
Relative Standard ◽  
Leucomalachite Green

A method for the determination of residues of malachite green and leucomalachite green in Chinese soft-shelled turtles (Pelodiscus sinensis) muscle by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The samples extracted with acetonitrile, and clean up on a neutral alumina SPE cartridge, separated on a C18 column using acetonitrile-5mmol/L ammonium acetate as mobile phase, and then detected under multiple reaction monitoring mode.The method showed a good linearity for all the analysts over the range of 0.1-50μg/L with r20.999.The detection limits of malachite green and leucomalachite green were 0.10 and 0.12μg/kg respectively. The recoveries ranged from 84.9 % to 93.8% at three spiked concentrations with the relative standard deviations lower than 5%. The results shows that this method has the advantages of easy to operate, fast to perform,with high sensitivity and accuracy, and it is suitable for detection of residues of malachite green and leucomalachite green in Chinese soft-shelled turtle (Pelodiscus sinensis).

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