WSN based on IPv6 is a new network integrated IPv6 and WSN. The related technologies of WSN based on IPv6 was researched, and an architecture of WSN based on IPv6 was proposed according to 6LoWPAN protocol in this article. Efficient and stable route protocol is a main focus to ensure network performance. Refer to on-demand routing protocol DSR, a Load-Balancing route protocol for WSN based on IPv6 was designed. An implementation of this protocol was programmed in NS2, and its simulation results were analyzed. The experimental result shows that this protocol could effectively reduce end-to-end delay and routing overhead, improving the network performance.