Different effects of titanium dioxide nanoparticles instillation in young and adult mice on DNA methylation related with lung inflammation and fibrosis

2019 ◽  
Vol 176 ◽  
pp. 1-10 ◽  
Author(s):  
Yue Ma ◽  
Yinsheng Guo ◽  
Hailing Ye ◽  
Kaiqin Huang ◽  
Ziquan Lv ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Titanium Dioxide ◽  
Lung Inflammation ◽  
Titanium Dioxide Nanoparticles ◽  
Adult Mice

scholarly journals Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on molecular mechanisms

2016 ◽  
Vol 7 ◽  
pp. 645-654 ◽  
Author(s):  
Bin Song ◽  
Yanli Zhang ◽  
Jia Liu ◽  
Xiaoli Feng ◽  
Ting Zhou ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Titanium Dioxide ◽  
Molecular Mechanisms ◽  
Titanium Dioxide Nanoparticles ◽  
Brain Dysfunction ◽  
In Vivo Studies ◽  
Cell Components ◽  
New Perspective ◽  
The Brain

Titanium dioxide nanoparticles (TiO2 NPs) possess unique characteristics and are widely used in many fields. Numerous in vivo studies, exposing experimental animals to these NPs through systematic administration, have suggested that TiO2 NPs can accumulate in the brain and induce brain dysfunction. Nevertheless, the exact mechanisms underlying the neurotoxicity of TiO2 NPs remain unclear. However, we have concluded from previous studies that these mechanisms mainly consist of oxidative stress (OS), apoptosis, inflammatory response, genotoxicity, and direct impairment of cell components. Meanwhile, other factors such as disturbed distributions of trace elements, disrupted signaling pathways, dysregulated neurotransmitters and synaptic plasticity have also been shown to contribute to neurotoxicity of TiO2 NPs. Recently, studies on autophagy and DNA methylation have shed some light on possible mechanisms of nanotoxicity. Therefore, we offer a new perspective that autophagy and DNA methylation could contribute to neurotoxicity of TiO2 NPs. Undoubtedly, more studies are needed to test this idea in the future. In short, to fully understand the health threats posed by TiO2 NPs and to improve the bio-safety of TiO2 NPs-based products, the neurotoxicity of TiO2 NPs must be investigated comprehensively through studying every possible molecular mechanism.

Effect of titanium dioxide nanoparticles on DNA methylation in multiple human cell lines

2020 ◽  
Vol 14 (4) ◽  
pp. 534-553 ◽  
Author(s):  
Marta Pogribna ◽  
Nathan A. Koonce ◽  
Ammu Mathew ◽  
Beverly Word ◽  
Anil K. Patri ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Titanium Dioxide ◽  
Cell Lines ◽  
Human Cell ◽  
Titanium Dioxide Nanoparticles ◽  
Human Cell Lines

scholarly journals Effects of Prenatal Exposure to Titanium Dioxide Nanoparticles on DNA Methylation and Gene Expression Profile in the Mouse Brain

2021 ◽  
Vol 3 ◽  
Author(s):  
Ken Tachibana ◽  
Shotaro Kawazoe ◽  
Atsuto Onoda ◽  
Masakazu Umezawa ◽  
Ken Takeda
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Titanium Dioxide ◽  
Mrna Expression ◽  
Cpg Island ◽  
Titanium Dioxide Nanoparticles ◽  
Cpg Islands ◽  
Female Offspring ◽  
Regulation Of Transcription ◽  
Male And Female

Background and Objectives: Titanium dioxide nanoparticles (TiO2-NP) are important materials used in commercial practice. Reportedly, TiO2-NP exposure during pregnancy can affect the development of the central nervous system in mouse offspring; however, the underlying mechanism remains unknown. In the present study, we investigated the impact of prenatal TiO2-NP exposure on global DNA methylation and mRNA expression patterns in the brains of neonatal mice.Materials and Methods: Pregnant C57BL/6J mice were intratracheally administered a TiO2-NP suspension (100 μg/mouse) on gestational day 10.5, and brains were collected from male and female offspring at day 1 postpartum. After extraction of methylated DNA by immunoprecipitation, the DNA methylation profile was analyzed using a mouse CpG island microarray. Total RNA was obtained, and mRNA expression profiles were comprehensively assessed using microarray analysis.Results: Among genes in the CpG island microarray, DNA methylation was increased in 614 and 2,924 genes and decreased in 6,220 and 6,477 genes in male and female offspring, respectively. Combined with mRNA microarray analysis, 88 and 89 genes were upregulated (≥1.5-fold) accompanied by demethylation of CpG islands, whereas 13 and 33 genes were downregulated (≤0.67-fold) accompanied by methylation of CpG islands in male and female offspring mice, respectively. Gene Set Enrichment Analysis (GSEA) revealed that these genes were enriched in gene ontology terms related to the regulation of transcription factors, cell proliferation, and organism development. Additionally, MeSH terms related to stem cells and morphogenesis were enriched.Conclusion: Prenatal TiO2-NP exposure induced genome-wide alterations in DNA methylation and mRNA expression in the brains of male and female offspring. Based on GSEA findings, it can be speculated that prenatal TiO2-NP exposure causes adverse effects on brain functions by altering the DNA methylation state of the fetal brain, especially neural stem cells, resulting in the subsequent abnormal regulation of transcription factors that modulate development and differentiation.

Titanium dioxide nanoparticles via oral exposure leads to adverse disturbance of gut microecology and locomotor activity in adult mice

2020 ◽  
Vol 94 (4) ◽  
pp. 1173-1190 ◽  
Author(s):  
Shanshan Zhang ◽  
Xuejun Jiang ◽  
Shuqun Cheng ◽  
Jingchuan Fan ◽  
Xia Qin ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Locomotor Activity ◽  
Titanium Dioxide Nanoparticles ◽  
Oral Exposure ◽  
Adult Mice
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