scholarly journals Evaluation of fasted state human intestinal fluid as apical solvent system in the Caco-2 absorption model and comparison with FaSSIF

2015 ◽  
Vol 67 ◽  
pp. 126-135 ◽  
Author(s):  
Benjamin Wuyts ◽  
Danny Riethorst ◽  
Joachim Brouwers ◽  
Jan Tack ◽  
Pieter Annaert ◽  
...  
Keyword(s):  
Solvent System ◽  
Absorption Model ◽  
Intestinal Fluid ◽  
Fasted State ◽  
Human Intestinal Fluid

Development and Validation of UV-Visible Spectrophotometric method for the Estimation of Curcumin and Tetrahydrocurcumin in Simulated Intestinal Fluid

2021 ◽  
pp. 2971-2975
Author(s):  
Jai Bharti Sharma ◽  
Sherry Sherry ◽  
Shailendra Bhatt ◽  
Vipin Saini ◽  
Manish Kumar
Keyword(s):  
Drug Release ◽  
Spectrophotometric Method ◽  
Tween 80 ◽  
Solvent System ◽  
Intestinal Fluid ◽  
Simulated Intestinal Fluid ◽  
Validation Parameters ◽  
Administration Method ◽  
Uv Visible ◽  
Development And Validation

Background: Due to solubility issues of curcumin and tetrahydrocurcumin, there is a need for the development of a UV-Visible spectrophotometric method that can estimate the drug release precisely and accurately. The addition of surfactant in the dissolution medium in low concentration achieved bio-comparable surface activity and can be used to estimate the drug release from formulations by avoiding sink conditions. Objective: The purpose of the present investigation was to develop a simple and précise UV-Visible spectrophotometric method for the determination of curcumin and tetrahydrocurcumin after oral administration. Method: A UV-Visible spectrophotometric method was developed using an appropriate solvent system for the estimation of curcumin and tetrahydrocurcumin. The solvent system having simulated intestinal fluid and particular concentration of surfactant was selected and further validated according to guidelines of the international conference on harmonization (ICH), the analytical parameter like linearity, precision and accuracy, etc. were studied. Results: Simulated intestinal fluid pH 7.4 with tween 80 at 1 % concentration satisfied all the conditions relative to peak quality at the stated wavelength for curcumin and intestinal fluid pH 7.4 with tween 80 at 0.5% concentration satisfied all the conditions relative to Peak quality at the stated wavelength for tetrahydrocurcumin. The developed methods were found within the range of all the validation parameters. Conclusion: The proposed method was found to be very simple and precise and can be used for routine quantitative analysis of curcumin and tetrahydrocurcumin.

scholarly journals Multidimensional analysis of human intestinal fluid composition

2020 ◽  
Vol 153 ◽  
pp. 226-240
Author(s):  
Kate Pyper ◽  
Joachim Brouwers ◽  
Patrick Augustijns ◽  
I. Khadra ◽  
C. Dunn ◽  
...  
Keyword(s):  
Multidimensional Analysis ◽  
Fluid Composition ◽  
Intestinal Fluid ◽  
Human Intestinal Fluid

An In-Depth View into Human Intestinal Fluid Colloids: Intersubject Variability in Relation to Composition

2016 ◽  
Vol 13 (10) ◽  
pp. 3484-3493 ◽  
Author(s):  
Danny Riethorst ◽  
Peter Baatsen ◽  
Caroline Remijn ◽  
Amitava Mitra ◽  
Jan Tack ◽  
...  
Keyword(s):  
Intersubject Variability ◽  
Intestinal Fluid ◽  
Human Intestinal Fluid ◽  
Depth View

Killing of Giardia lamblia Trophozoites by Human Intestinal Fluid in Vitro

1988 ◽  
Vol 157 (6) ◽  
pp. 1257-1260 ◽  
Author(s):  
S. Das ◽  
D. S. Reiner ◽  
J. Zenian ◽  
D. L. Hogan ◽  
M. A. Koss ◽  
...  
Keyword(s):  
Giardia Lamblia ◽  
Intestinal Fluid ◽  
Human Intestinal Fluid

scholarly journals Simultaneous assessment of lipid classes and bile acids in human intestinal fluid by solid-phase extraction and HPLC methods

2006 ◽  
Vol 48 (1) ◽  
pp. 242-251 ◽  
Author(s):  
Eva Persson ◽  
Lars Löfgren ◽  
Göran Hansson ◽  
Bertil Abrahamsson ◽  
Hans Lennernäs ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Bile Acids ◽  
Solid Phase ◽  
Lipid Classes ◽  
Phase Extraction ◽  
Intestinal Fluid ◽  
Human Intestinal Fluid ◽  
Simultaneous Assessment

Equilibrium drug solubility measurements in 96-well plates reveal similar drug solubilities in phosphate buffer pH 6.8 and human intestinal fluid

2011 ◽  
Vol 405 (1-2) ◽  
pp. 132-136 ◽  
Author(s):  
Tiina Heikkilä ◽  
Milja Karjalainen ◽  
Krista Ojala ◽  
Kirsi Partola ◽  
Frank Lammert ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Drug Solubility ◽  
Intestinal Fluid ◽  
Similar Drug ◽  
Human Intestinal Fluid

Modifying release of poorly soluble active pharmaceutical ingredients with the amine functionalized SBA-16 type mesoporous materials

2019 ◽  
Vol 33 (9) ◽  
pp. 1214-1231 ◽  
Author(s):  
Barbara Jadach ◽  
Agnieszka Feliczak-Guzik ◽  
Izabela Nowak ◽  
Bartłomiej Milanowski ◽  
Hanna Piotrowska-Kempisty ◽  
...  
Keyword(s):  
Mesoporous Materials ◽  
Gastric Fluid ◽  
Bet Surface Area ◽  
Simulated Gastric Fluid ◽  
Active Pharmaceutical Ingredients ◽  
Intestinal Fluid ◽  
Pharmaceutical Ingredients ◽  
Fasted State ◽  
Small Intestinal ◽  
Release Profiles

SBA-16 and two modified SBA-16 type ordered mesoporous silica were used as the carriers for ibuprofen (anti-inflammatory drug) and furosemide (loop diuretic drug). Modification of the solid carrier was prepared with chitosan or N-3[(amino(poly-propylenoxy)]aminopropyltrimethoxysilane. The samples of carriers and carrier-drug loaded materials were characterized by X-ray diffraction, N2 adsorption, Fourier-transform infrared spectroscopy, thermogravimetry, and differential scanning calorimetry. The release profiles of active pharmaceutical ingredients were performed in media with different pH in the USP 2 apparatus as well as in two biorelevant media (fasted state simulated gastric fluid and fasted state small intestinal fluid) in USP 4 apparatus. The loading of active substances into mesoporous materials was performed with modified immersion method. The maximum content of deposited drug in mesoporous material was close to 12.0 and 2.2 wt.% for ibuprofen and furosemide, respectively. After drug adsorption, the reduction of BET surface area, pore volume and pore diameter of non-modified and modified SBA-16 was observed, while the cubic arrays of siliceous matrix were well preserved. The release profiles of ibuprofen and furosemide loaded in mesoporous materials in media with different pH and biorelevant fasted state simulated gastric fluid and fasted state small intestinal fluid showed that the new SBA-16 type materials modify the release profiles of furosemide, increasing the dissolution rate of these substances in the medium at pH 1.2. The cytotoxicity of the materials and permeability of drugs after their loading on SBA-16 materials were evaluated on Caco-2 model. The results of our study showed that mesoporous materials did not exert cytotoxic effects and did not influence on the permeability of both active pharmaceutical ingredients in relation to pure substances.

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