Tailoring a hybrid intelligent model to predict fermentable sugar production from enzyme−catalyzed hydrolysis of damaged wheat grains

ABSTRACTMarine red macroalgae have emerged to be renewable biomass for the production of chemicals and biofuels, because carbohydrates that form the major component of red macroalgae can be hydrolyzed into fermentable sugars. The main carbohydrate in red algae is agarose, and it is composed ofd-galactose and 3,6-anhydro-l-galactose (AHG), which are alternately bonded by β1-4 and α1-3 linkages. In this study, a novel β-galactosidase that can act on agarooligosaccharides (AOSs) to release galactose was discovered in a marine bacterium (Vibriosp. strain EJY3); the enzyme is annotated asVibriosp. EJY3 agarolytic β-galactosidase (VejABG). Unlike thelacZ-encoded β-galactosidase fromEscherichia coli,VejABG does not hydrolyze common substrates like lactose and can act only on the galactose moiety at the nonreducing end of AOS. The optimum pH and temperature ofVejABG on an agarotriose substrate were 7 and 35°C, respectively. Its catalytic efficiency with agarotriose was also similar to that with agaropentaose or agaroheptaose. Since agarotriose lingers as the unreacted residual oligomer in the currently available saccharification system using β-agarases and acid prehydrolysis, the agarotriose-hydrolyzing capability of this novel β-galactosidase offers an enormous advantage in the saccharification of agarose or agar in red macroalgae for its use as a biomass feedstock for fermentable sugar production.

Download Full-text

Enzymatic hydrolysis of lignocellulosic biomass in ionic liquid media for fermentable sugar production

Journal of the Taiwan Institute of Chemical Engineers ◽

10.1016/j.jtice.2012.02.001 ◽

2012 ◽

Vol 43 (4) ◽

pp. 573-577 ◽

Cited By ~ 19

Author(s):

Chia-Hung Su ◽

Ming-Hua Chung ◽

Hsin-Ju Hsieh ◽

Yu-Kaung Chang ◽

Jin-Chau Ding ◽

...

Keyword(s):

Ionic Liquid ◽

Enzymatic Hydrolysis ◽

Lignocellulosic Biomass ◽

Sugar Production ◽

Fermentable Sugar ◽

Liquid Media ◽

Hydrolysis Of

Download Full-text

Upgrading brewer's spent grains by treatment with Aspergillus species

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq0702072b ◽

2007 ◽

Vol 13 (2) ◽

pp. 72-78 ◽

Cited By ~ 6

Author(s):

Argyro Bekatorou ◽

Yiannis Bountas ◽

Ibrahim Banat ◽

Maria Kanellakl

Keyword(s):

Enzyme Stability ◽

Carbon Sources ◽

Growth Media ◽

Sugar Production ◽

Fermentable Sugar ◽

Yeast Growth ◽

First Case ◽

Spent Grains ◽

Biomass Yields ◽

Hydrolysis Of

Hydrolysis of brewer's spent grains (BSG) starch was examined using Aspergillus oryzae and A. awamori, at various conditions (pH, spore concentrations). Both fungi performed well, although A. oryzae proved more efficient in terms of process times and enzyme stability. BSG slurries were either treated directly with spore suspensions at 30?C, or with crude enzyme solutions at 45?C. In the first case, simultaneous biomass production and starch hydrolysis occurred, but in the second, transformation of sugars to biomass was avoided. In both cases, fermentable sugar production was not efficient (0.24-0.95 g/l and 0.47-1.83 g/l respectively) to support the use of the BSG hydrolyzates (BSGHs) as substrates e.g. for yeast propagation within the brewery. Alternatively, BSG treated directly with fungi could be proposed as protein enriched animal feeds. BSGHs were also evaluated as nutritious supplements in yeast growth media containing mixtures of molasses and orange pulp (as carbon sources), resulting in significantly improved biomass yields.

Download Full-text