Extraction of pectin from agroindustrial residue with an ecofriendly solvent: use of FTIR and chemometrics to differentiate pectins according to degree of methyl esterification

2020 ◽  
Vol 107 ◽  
pp. 105921 ◽  
Author(s):  
Emylle Emediato Santos ◽  
Rodrigo Chaves Amaro ◽  
Constanza Catarina Cid Bustamante ◽  
Milton Hércules Andrade Guerra ◽  
Liliane Catone Soares ◽  
...  
Catalysts ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 677
Author(s):  
John Onolame Unuofin

Laccase is increasingly adopted in diverse industrial and environmental applications, due to its readily accessible requirements for efficient catalytic synthesis and biotransformation of chemicals. However, it is perceived that its industrial production might incur some unfavorable overhead, which leads to expensive market products, and the corresponding negative environmental feedback, due to the use of capital-intensive and precarious chemicals. To this end, this study was designed to evaluate the performance indicators of the valorization of wheat bran by a novel Jb1b laccase and its subsequent application in waste minimization and water management, on a laboratory scale. Optimal Jb1b laccase was produced in submerged fermentation medium containing wheat bran, an agroindustrial residue, through response surface methodology (RSM) algorithm, and was applied in dye decolorization and denim bioscouring, respectively. Results showed that the resultant enzyme manifested unique biochemical properties, such as enhanced tolerance at certain physicochemical conditions, with a residual activity of at least ca. 76%. Furthermore, phenomenally high concentrations of synthetic dyes (0.2% w v−1) were decolorized over 56 h, and a 6 h mediator-supported simultaneous denim bleaching and decolorization of wash effluent was observed. The sustainability of the production and application processes were inferred from the reusability of the fermentation sludge as a potential biofertilizer, with subsequent prospects for the biostimulation and bioaugmentation of contaminated soils, whereas the decolorized water could be adopted for other uses, amongst which horticulture and forestry are typical examples. These phenomena therefore authenticate the favorable environmental feedbacks and overhead realized in this present study.


2004 ◽  
Vol 18 (4) ◽  
pp. 665-668 ◽  
Author(s):  
M.M.H Huisman ◽  
A Oosterveld ◽  
H.A Schols

Microbiology ◽  
2006 ◽  
Vol 152 (3) ◽  
pp. 617-625 ◽  
Author(s):  
Margarita Soriano ◽  
Pilar Diaz ◽  
Francisco I. Javier Pastor

The gene yvpA from Bacillus subtilis was cloned and expressed in Escherichia coli. It encoded a pectate lyase of 221 amino acids that was denominated PelC. The heterologously expressed enzyme was purified by His-tag affinity chromatography and characterized. PelC depolymerized polygalacturonate and pectins of methyl esterification degree from 22 % to 89 %, exhibiting maximum activity on 22 % esterified citrus pectin. It showed an absolute Ca2+ requirement and the optimum temperature and pH were 65 °C and pH 10, respectively. The deduced amino acid sequence of PelC showed 53 % identity to pectate lyase PelA from Paenibacillus barcinonensis, which was also characterized. Similarly to PelC, purified PelA showed activity on polygalacturonate and pectins with a high degree of methyl esterification. The two enzymes cleaved pectic polymers to a mixture of oligogalacturonates, indicating an endo mode of action. Analysis of activity on trigalacturonate showed that PelC cleaved it to galacturonic acid and unsaturated digalacturonate, whereas PelA did not show activity on this substrate. PelC and PelA showed high homology to a few recently identified pectate lyases of family 3 and form with them a cluster of small-sized pectate lyases from non-pathogenic micro-organisms.


2011 ◽  
Vol 31 (1) ◽  
pp. 40-46 ◽  
Author(s):  
KYLIE M. DINGWALL ◽  
PAUL MARUFF ◽  
ALAN R. CLOUGH ◽  
SHEREE CAIRNEY

Química Nova ◽  
2012 ◽  
Vol 35 (8) ◽  
pp. 1510-1516 ◽  
Author(s):  
Swamy Arêa Maruyama ◽  
Fabio da Silva Lisboa ◽  
Luiz Pereira Ramos ◽  
Fernando Wypych

2008 ◽  
Vol 377 (2) ◽  
pp. 234-242 ◽  
Author(s):  
Eric S. Simon ◽  
Matthew Young ◽  
Antonia Chan ◽  
Zhao-Qin Bao ◽  
Philip C. Andrews

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