Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus

Fuel ◽  
2009 ◽  
Vol 88 (9) ◽  
pp. 1602-1607 ◽  
Author(s):  
Svetlana Nikolić ◽  
Ljiljana Mojović ◽  
Marica Rakin ◽  
Dušanka Pejin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immobilized Cells ◽  
Enzymatic Saccharification ◽  
Bioethanol Production ◽  
Corn Meal

Bioethanol Production from Rice Straw in the MAFF Research Project, Japan

2009 ◽  
Vol 1219 ◽  
Author(s):  
Ken Tokuyasu
Keyword(s):  
Heat Treatment ◽  
Saccharomyces Cerevisiae ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Bioethanol Production ◽  
Chemical Pretreatment ◽  
Simple Method ◽  
Herbaceous Biomass ◽  
Free Glucose ◽  
Simultaneous Saccharification

AbstractRice straw is among the most abundant herbaceous biomass, and regarded as the central feedstock for bioethanol production in Japan. We found that significant amounts of soft carbohydrates (SCs), defined as carbohydrates readily recoverable by mere extraction from the biomass or brief enzymatic saccharification, exist in rice straw in the form of free glucose, free fructose, sucrose, starch, and β-1,3-1,4-glucan. Based on the finding, we proposed a simple method for bioethanol production from rice straw samples with SCs, by a heat treatment for sterilization and starch gelatinization, followed by simultaneous saccharification/fermentation with Saccharomyces cerevisiae. This method would offer an efficient process for bioethanol production without the aid of harsh thermo/chemical pretreatment step.

scholarly journals IMPROVEMENT OF BIOETHANOL PRODUCTION BY USING Saccharomyces cerevisiae [Meyen ex E.C. Hansen] IMMOBILIZED ON PRETREATED SUGARCANE BAGASSE

REAKTOR ◽  
2018 ◽  
Vol 18 (03) ◽  
pp. 127 ◽  
Author(s):  
Sita Heris Anita ◽  
Wibowo Mangunwardoyo ◽  
Yopi Yopi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Physical Properties ◽  
Sugarcane Bagasse ◽  
Immobilized Cells ◽  
Ethanol Yield ◽  
Steam Treatment ◽  
Bioethanol Production ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Pretreated Sugarcane Bagasse

Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and combination both of procedure.  The objectives of this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production.  Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension.  Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation.  The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes.  Those treatment improved the physical properties of carrier and increased the cell retention up to 10.05 mg/g.  The use of whole cell biocatalyst after steaming pretreatment also enhanced ethanol yield 1.5 times higher than control. Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse

Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus

2010 ◽  
Vol 34 (10) ◽  
pp. 1449-1456 ◽  
Author(s):  
Svetlana Nikolić ◽  
Ljiljana Mojović ◽  
Dušanka Pejin ◽  
Marica Rakin ◽  
Maja Vukašinović
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immobilized Cells ◽  
Corn Meal

Bioethanol production from mixed sugars by Scheffersomyces stipitis free and immobilized cells, and co-cultures with Saccharomyces cerevisiae

2013 ◽  
Vol 30 (6) ◽  
pp. 591-597 ◽  
Author(s):  
Isabella De Bari ◽  
Paola De Canio ◽  
Daniela Cuna ◽  
Federico Liuzzi ◽  
Angela Capece ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immobilized Cells ◽  
Bioethanol Production ◽  
Scheffersomyces Stipitis ◽  
Mixed Sugars

scholarly journals Transformation of lignocellulose from corn stove for bioethanol production

2021 ◽  
Vol 3 (1) ◽  
pp. 44-48
Author(s):  
Numchok Manmai ◽  
Yuwalee Unpaprom ◽  
Ramaeshprabu Ramaraj ◽  
Keng-Tung Wu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fossil Fuels ◽  
Renewable Energy Sources ◽  
Enzymatic Saccharification ◽  
Environmental Issues ◽  
Energy Sources ◽  
Bioethanol Production ◽  
Fermentation Time ◽  
Separate Hydrolysis And Fermentation ◽  
Petroleum Fuels

The use of fossil fuels, as well as the environmental issues associated with their burning, has pushed for the development of clean, renewable energy sources. Biofuels made from lignocellulosic biomass are considered a carbon-neutral and sustainable method. As the demand for non-petroleum fuels grows, more attention will be placed on developing a cost-competitive liquid transportation biofuel like ethanol. This study was conducted to produce bioethanol utilizing the SHF (separate hydrolysis and fermentation) technique from corn stove lignocellulose. Pretreatment with sodium hydroxide at various concentrations was also studied. The influence of enzymatic saccharification, fermentation time, and substrate concentration on sugar yield and, eventually, ethanol production was investigated. Fermentation was carried out by using the enzymatically saccharified hydrolysate and monoculture of Saccharomyces cerevisiae. The results reveal that pretreatment with 2% NaOH followed by 48 hours of hydrolysis produced the maximum bioethanol production (30.21 ±0.13 g/L). This study findings indicated that alkali-pretreated corn stove might be used as a feedstock for bioethanol production, reducing reliance on fossil fuels.

scholarly journals Improved Glucose Recovery from Sicyos angulatus by NaOH Pretreatment and Application to Bioethanol Production

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 245
Author(s):  
Hyung-Eun An ◽  
Kang Hyun Lee ◽  
Ye Won Jang ◽  
Chang-Bae Kim ◽  
Hah Young Yoo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alternative Energy ◽  
Invasive Plant ◽  
Food Resource ◽  
Raw Material ◽  
Control Group ◽  
Bioethanol Production ◽  
Naoh Pretreatment ◽  
Glucose Recovery ◽  
Harmful Species

As greenhouse gases and environmental pollution become serious, the demand for alternative energy such as bioethanol has rapidly increased, and a large supply of biomass is required for bioenergy production. Lignocellulosic biomass is the most abundant on the planet and a large part of it, the second-generation biomass, has the advantage of not being a food resource. In this study, Sicyos angulatus, known as an invasive plant (harmful) species, was used as a raw material for bioethanol production. In order to improve enzymatic hydrolysis, S. angulatus was pretreated with different NaOH concentration at 121 °C for 10 min. The optimal NaOH concentration for the pretreatment was determined to be 2% (w/w), and the glucan content (GC) and enzymatic digestibility (ED) were 46.7% and 55.3%, respectively. Through NaOH pretreatment, the GC and ED of S. angulatus were improved by 2.4-fold and 2.5-fold, respectively, compared to the control (untreated S. angulatus). The hydrolysates from S. angulatus were applied to a medium for bioethanol fermentation of Saccharomyces cerevisiae K35. Finally, the maximum ethanol production was found to be 41.3 g based on 1000 g S. angulatus, which was 2.4-fold improved than the control group.

scholarly journals Mead Production Using Immobilized Cells of Saccharomyces cerevisiae: Reuse of Sodium Alginate Beads

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 724
Author(s):  
Miguel L. Sousa-Dias ◽  
Vanessa Branco Paula ◽  
Luís G. Dias ◽  
Letícia M. Estevinho
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sodium Alginate ◽  
Fermentation Process ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Production Medium ◽  
Alcohol Content ◽  
Volatile Acidity ◽  
The Matrix ◽  
Second Category

This work studied the production of mead using second category honey and the immobilized cells of Saccharomyces cerevisiae in sodium alginate, with concentrations of 2% and 4%, and their reuse in five successive fermentations. The immobilized cells with 4% alginate beads were mechanically more stable and able to allow a greater number of reuses, making the process more economical. The fermentation’s consumption of sugars with free cells (control) and immobilized cells showed a similar profile, being completed close to 72 h, while the first use of immobilized cells finished at 96 h. The immobilized cells did not significantly influence some oenological parameters, such as the yield of the consumed sugars/ethanol, the alcohol content, the pH and the total acidity. There was a slight increase in the volatile acidity and a decrease in the production of SO2. The alginate concentrations did not significantly influence either the parameters used to monitor the fermentation process or the characteristics of the mead. Mead fermentations with immobilized cells showed the release of cells into the wort due to the disintegration of the beads, indicating that the matrix used for the yeast’s immobilization should be optimized, considering the mead production medium.

scholarly journals Efficient utilization of date palm waste for the bioethanol production through Saccharomyces cerevisiae strain

2021 ◽  
Vol 9 (4) ◽  
pp. 2066-2074
Author(s):  
Arslan Ahmad ◽  
Summar A. Naqvi ◽  
Muhammad J. Jaskani ◽  
Muhammad Waseem ◽  
Ehsan Ali ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Date Palm ◽  
Bioethanol Production ◽  
Efficient Utilization ◽  
Date Palm Waste ◽  
Palm Waste
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