scholarly journals Molecular Genetic Diversity of Winged Bean Gene Pool in Thailand Assessed by SSR Markers

2021 ◽  
Author(s):  
Kularb Laosatit ◽  
Kitiya Amkul ◽  
Sompong Chankaew ◽  
Prakit Somta
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Gene Pool ◽  
Molecular Genetic ◽  
Winged Bean ◽  
Molecular Genetic Diversity

Molecular genetic diversity and population structure in Lycium accessions using SSR markers

2010 ◽  
Vol 333 (11-12) ◽  
pp. 793-800 ◽  
Author(s):  
Wei-Guo Zhao ◽  
Jong-Wook Chung ◽  
Young-Il Cho ◽  
Won-Hee Rha ◽  
Gi-An Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers ◽  
Molecular Genetic ◽  
Molecular Genetic Diversity

scholarly journals Genetic diversity and SSR markers development of ancient Camellia sinensis in Sandu County of Guizhou Province

2021 ◽  
Author(s):  
Yichen Zhao ◽  
Runying Wang ◽  
Qing Liu ◽  
Xuan Dong ◽  
De-Gang Zhao
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Diversity Index ◽  
Molecular Genetic ◽  
Diversity Indices ◽  
Guizhou Province ◽  
Information Index ◽  
Molecular Genetic Diversity ◽  
Qualitative Characters ◽  
Breed Selection

Abstract Background: The genetic abundance of ancient tea germplasm has been preserved in the long evolution process, which provides valuable resources for the protection and breed selection of ancient tea germplasm. However, the limited studies related to the genetic diversity of ancient tea germplasm restrict protection and breed selection. Therefore, the genetic diversity of ancient tea germplasm in Sandu county of Guizhou Province was explored in this study. II.Methods and Results: The genetic diversity was analyzed using phenotypes and SSR markers. The ranges for the variation coefficients of the six quantitative and seven qualitative characters were 17.76%-60.37% and 18.58%-50.64%, respectively. The ranges of diversity indices of the six quantitative and seven qualitative characters were 1.72-2.74 and 0.55-0.84, respectively. Ninety-six bands were amplified using 15 pairs of SSR primers from the 145 samples, and the average polymorphism information index was 0.66. The analysis revealed that the average values of Nei’s genetic diversity index (H) and the Shannon information index (I) are 0.26 and 0.41, respectively. Further, a genetic similarity coefficient of 0.734 shown by UPGMA dendrogram classified the 145 samples decreased into four groups. III.Conclusions: This study revealed the rich phenotypic variation and high molecular genetic diversity and the genetic diversity of the arbor is higher than that of the shrub of the ancient tea germplasm in Sandu of Guizhou province. Thus, this study not only provides a theoretical basis for the protection and breed selection but also promotes further research of ancient tea germplasm.

scholarly journals Molecular Genetic Diversity and Population Structure in Ethiopian Chickpea Germplasm Accessions

Diversity ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 247
Author(s):  
Tsegaye Getahun ◽  
Kassahun Tesfaye ◽  
Asnake Fikre ◽  
Teklehaimanot Haileslassie ◽  
Annapurna Chitikineni ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers ◽  
Molecular Genetic ◽  
Conservation Strategies ◽  
Molecular Genetic Diversity ◽  
Population Structure Analysis ◽  
Trait Improvement ◽  
Population Structures ◽  
Germplasm Accessions

Chickpea (Cicer arietinum L.) is a cheap source of protein and rich in minerals for people living in developing countries. In order to assess the existing molecular genetic diversity and determine population structures in selected Ethiopian chickpea germplasm accessions (118), a set of 46 simple sequence repeat (SSR) markers equally distributed on the chickpea genome were genotyped. A total of 572 alleles were detected from 46 SSR markers, and the number of alleles per locus varied from 2 (ICCM0289) to 28 (TA22). The average number of alleles per locus, polymorphism information content, and expected heterozygosity were 12, 0.684, and 0.699, respectively. Phylogenetic analysis grouped the 118 chickpea genotypes from diverse sources into three evolutionary and/or biological groups (improved desi, improved kabuli, and landraces). The population structure analysis revealed six sub-populations from 118 chickpea genotypes studied. AMOVA revealed that 57%, 29%, and 14% of the total genetic variations were observed among individuals, within populations, and among populations. The insights into the genetic diversity at molecular levels in the Ethiopian germplasm lines can be used for designing conservation strategies as well as the diverse germplasm lines identified in this study can be used for trait dissection and trait improvement.

Molecular genetic diversity and linked resistance to ascochyta blight in Australian chickpea breeding materials and their wild relatives

2008 ◽  
Vol 59 (6) ◽  
pp. 554 ◽  
Author(s):  
M. Imtiaz ◽  
M. Materne ◽  
K. Hobson ◽  
M. van Ginkel ◽  
R. S. Malhotra
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Genetic Similarity ◽  
Polymorphic Information Content ◽  
Molecular Genetic ◽  
Ascochyta Blight ◽  
Wild Relatives ◽  
Pedigree Information ◽  
Common Source ◽  
Molecular Genetic Diversity

Simple sequence-repeat (SSR) and sequence characterised amplified regions (SCARs) have been used to characterise the genetic diversity of chickpea germplasm. A set of 48 genotypes comprising cultigen, landraces, and wild relatives important for breeding purposes was used to determine the genetic similarity between genotypes and to assess the association between ascochyta blight (AB) and SCAR phenotypes. The 21 SSR markers amplified a total of 370 alleles, with an average of ~17 alleles per SSR locus among the 48 genotypes. Polymorphic information content (PIC) values ranged from 0.37 for the XGA13 locus to 0.93 for the XGA106. Principal coordinate analysis (PCO) of genetic similarity (GS) estimates revealed a clear differentiation of the chickpea genotypes into 5 groups, which were generally consistent with available pedigree information. Comparison of SCAR and AB phenotypes enabled us to tag the common source(s) of AB resistance in the breeding collection. Based on the SCAR phenotypes, it was evident that the studied chickpea genotypes, including worldwide-known AB-resistant lines (ICC12004, ILC72, ILC3279), carry at least one common source of resistance to AB. Since SSR markers are polymerase chain reaction (PCR)-based markers, highly polymorphic, and amenable to high-throughput technologies, they are therefore well suited for studies of genetic diversity and cultivar identification in chickpea. The broad level of genetic diversity detected in the chickpea germplasm should be useful for selective breeding for specific traits such as AB, backcrossing, and in enhancing the genetic base of breeding programs.

Development and use of novel SSR markers for molecular genetic diversity in Italian millet (Setaria italica L.)

2012 ◽  
Vol 34 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Weiguo Zhao ◽  
Gi-An Lee ◽  
Soon-Wook Kwon ◽  
Kyung-Ho Ma ◽  
Myung-Chul Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Molecular Genetic ◽  
Setaria Italica ◽  
Molecular Genetic Diversity
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