Hyaluronic acid hydrogels, as a biological macromolecule-based platform for stem cells delivery and their fate control: A review

2021 ◽  
Vol 189 ◽  
pp. 554-566
Author(s):  
Yu Fang ◽  
Lele Shi ◽  
Zhiwei Duan ◽  
Saeed Rohani
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Biological Macromolecule ◽  
Fate Control

scholarly journals Biotechnology Applied to Cosmetics and Aesthetic Medicines

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 33
Author(s):  
Cátia Gomes ◽  
Ana Catarina Silva ◽  
Ana Camila Marques ◽  
José Sousa Lobo ◽  
Maria Helena Amaral
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Growth Factors ◽  
Genetic Engineering ◽  
Kojic Acid ◽  
Active Ingredients ◽  
High Quality ◽  
Vast Number ◽  
Biotechnological Processes

Biotechnology uses microorganisms and/or enzymes to obtain specific products through fermentative processes and/or genetic engineering techniques. Examples of these products are active ingredients, such as hyaluronic acid, kojic acid, resveratrol, and some enzymes, which are used in skin anti-aging products. In addition, certain growth factors, algae, stem cells, and peptides have been included in cosmetics and aesthetic medicines. Thus, biotechnology, cosmetics and aesthetic medicines are now closely linked, through the production of high-quality active ingredients, which are more effective and safer. This work describes the most used active ingredients that are produced from biotechnological processes. Although there are a vast number of active ingredients, the number of biotechnological active ingredients reported in the literature is not significantly high.

Evaluation of the Effect of Hyaluronic Acid–Based Biomaterial Enriched With Tenascin-C on the Behavior of the Neural Stem Cells

2021 ◽  
pp. 109158182110024
Author(s):  
Maryam Shahi ◽  
Daruosh Mohammadnejad ◽  
Mohammad Karimipour ◽  
Reza Rahbarghazi ◽  
Ali Abedelahi
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Neural Stem Cells ◽  
Mtt Assay ◽  
Dapi Staining ◽  
Biological Assays ◽  
Tenascin C ◽  
Electron Scanning Microscopy ◽  
Ethidium Bromide Staining ◽  
And Control

One of the most important natural extracellular constituents is hyaluronic acid (HA) with the potential to develop a highly organized microenvironment. In the present study, we enriched HA hydrogel with tenascin-C (TN-C) and examined the viability and survival of mouse neural stem cells (NSCs) using different biological assays. Following NSCs isolation and expansion, their phenotype was identified using flow cytometry analysis. Cell survival was measured using MTT assay and DAPI staining after exposure to various concentrations of 50, 100, 200, and 400 nM TN-C. Using acridine orange/ethidium bromide staining, we measured the number of live and necrotic cells after exposure to the combination of HA and TN-C. MTT assay revealed the highest NSCs viability rate in the group exposed to 100 nM TN-C compared to other groups, and a combination of 1% HA + 100 nM TN-C increased the viability of NSCs compared to the HA group after 24 hours. Electron scanning microscopy revealed the higher attachment of these cells to the HA + 100 nM TN-C substrate relative to the HA substrate. Epifluorescence imaging and DAPI staining of loaded cells on HA + 100 nM TN-C substrate significantly increased the number of NSCs per field over 72 hours compared to the HA group ( P < 0.05). Live and dead assay revealed that the number of live NSCs significantly increased in the HA + 100 TN-C group compared to HA and control groups. The enrichment of HA substrate with TN-C promoted viability and survival of NSCs and could be applied in neural tissue engineering approaches and regenerative medicine.

scholarly journals Controlling the adhesion and differentiation of mesenchymal stem cells using hyaluronic acid-based, doubly crosslinked networks

Biomaterials ◽  
2011 ◽  
Vol 32 (10) ◽  
pp. 2466-2478 ◽  
Author(s):  
Amit K. Jha ◽  
Xian Xu ◽  
Randall L. Duncan ◽  
Xinqiao Jia
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Crosslinked Networks

Bioinspired mineral-polymeric hybrid hyaluronic acid/ poly (γ-glutamic acid) hydrogels as tunable scaffolds for stem cells differentiation

2021 ◽  
pp. 118048
Author(s):  
Shuai Liu ◽  
Peili Li ◽  
Xin Liu ◽  
Penghui Wang ◽  
Wenliang Xue ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Glutamic Acid

Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid – Gelatin injectable hydrogels

2020 ◽  
Vol 134 ◽  
pp. 107536 ◽  
Author(s):  
Juan Jairo Vaca-González ◽  
Sandra Clara-Trujillo ◽  
María Guillot-Ferriols ◽  
Joaquín Ródenas-Rochina ◽  
María J. Sanchis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Hyaluronic Acid ◽  
Chondrogenic Differentiation ◽  
Injectable Hydrogels ◽  
Cells Cultured

scholarly journals PTMAc-PEG-PTMAc hydrogel modified by RGDC and hyaluronic acid promotes neural stem cells' survival and differentiation in vitro

RSC Advances ◽  
2017 ◽  
Vol 7 (65) ◽  
pp. 41098-41104 ◽  
Author(s):  
Ruirui Yang ◽  
Caixia Xu ◽  
Tao Wang ◽  
Yuanqi Wang ◽  
Jingnan Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Central Nervous System ◽  
Nervous System ◽  
Hyaluronic Acid ◽  
Neural Stem Cells ◽  
Biological Properties ◽  
Central Nervous System Injury ◽  
Bioactive Molecules

The enhancement of the biological properties of hydrogels by surface modifying with bioactive molecules is of great significance, especially for the treatment of central nervous system injury by combining engrafted cells.

Peptide-conjugated hyaluronic acid surface for the culture of human induced pluripotent stem cells under defined conditions

2016 ◽  
Vol 136 ◽  
pp. 1061-1064 ◽  
Author(s):  
Xiaohong Zhang ◽  
Ping Zhou ◽  
Yinghui Zhao ◽  
Mengke Wang ◽  
Shicheng Wei
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent
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