scholarly journals The Biochemistry and Fidelity of Synthesis by the Apicoplast Genome Replication DNA Polymerase Pfprex from the Malaria Parasite Plasmodium falciparum

2011 ◽  
Vol 410 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Scott R. Kennedy ◽  
Cheng-Yao Chen ◽  
Michael W. Schmitt ◽  
Cole N. Bower ◽  
Lawrence A. Loeb
Keyword(s):  
Plasmodium Falciparum ◽  
Dna Polymerase ◽  
Malaria Parasite ◽  
Genome Replication ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum ◽  
Apicoplast Genome

scholarly journals Tetracyclines Specifically Target the Apicoplast of the Malaria Parasite Plasmodium falciparum

2006 ◽  
Vol 50 (9) ◽  
pp. 3124-3131 ◽  
Author(s):  
Erica L. Dahl ◽  
Jennifer L. Shock ◽  
Bhaskar R. Shenai ◽  
Jiri Gut ◽  
Joseph L. DeRisi ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Malaria Parasite ◽  
Life Cycles ◽  
Mitochondrial Genomes ◽  
Mitochondrial Morphology ◽  
Genome Replication ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum ◽  
Specific Activities ◽  
Apicoplast Genome

ABSTRACT Tetracyclines are effective but slow-acting antimalarial drugs whose mechanism of action remains uncertain. To characterize the antimalarial mechanism of tetracyclines, we evaluated their stage-specific activities, impacts on parasite transcription, and effects on two predicted organelle targets, the apicoplast and the mitochondrion, in cultured Plasmodium falciparum. Antimalarial effects were much greater after two 48-h life cycles than after one cycle, even if the drugs were removed at the end of the first cycle. Doxycycline-treated parasites appeared morphologically normal until late in the second cycle of treatment but failed to develop into merozoites. Doxycycline specifically impaired the expression of apicoplast genes. Apicoplast morphology initially appeared normal in the presence of doxycycline. However, apicoplasts were abnormal in the progeny of doxycycline-treated parasites, as evidenced by a block in apicoplast genome replication, a lack of processing of an apicoplast-targeted protein, and failure to elongate and segregate during schizogeny. Replication of the nuclear and mitochondrial genomes and mitochondrial morphology appeared normal. Our results demonstrate that tetracyclines specifically block expression of the apicoplast genome, resulting in the distribution of nonfunctional apicoplasts into daughter merozoites. The loss of apicoplast function in the progeny of treated parasites leads to a slow but potent antimalarial effect.

scholarly journals Interactions between Merozoite Surface Proteins 1, 6, and 7 of the Malaria Parasite Plasmodium falciparum

2006 ◽  
Vol 281 (42) ◽  
pp. 31517-31527
Author(s):  
Christian W. Kauth ◽  
Ute Woehlbier ◽  
Michaela Kern ◽  
Zeleke Mekonnen ◽  
Rolf Lutz ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Malaria Parasite ◽  
Surface Proteins ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum

The plastid DNA of the malaria parasite Plasmodium falciparum is replicated by two mechanisms

2002 ◽  
Vol 45 (2) ◽  
pp. 533-542 ◽  
Author(s):  
D. H. Williamson ◽  
P. R. Preiser ◽  
P. W. Moore ◽  
S. McCready ◽  
M. Strath ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Malaria Parasite ◽  
Plastid Dna ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum
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