Maternal consumption of low-isoflavone soy protein isolate alters hepatic gene expression and liver development in rat offspring

2017 ◽  
Vol 42 ◽  
pp. 51-61 ◽  
Author(s):  
Sae Bom Won ◽  
Anna Han ◽  
Young Hye Kwon
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Liver Development ◽  
Hepatic Gene Expression ◽  
Rat Offspring ◽  
Maternal Consumption

Intake of Soy Protein Isolate Alters Hepatic Gene Expression in Rats

2005 ◽  
Vol 53 (10) ◽  
pp. 4253-4257 ◽  
Author(s):  
Nobuhiko Tachibana ◽  
Ichiro Matsumoto ◽  
Kensuke Fukui ◽  
Soichi Arai ◽  
Hisanori Kato ◽  
...  
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Hepatic Gene Expression

scholarly journals Hepatic gene expression following consumption of soy protein isolate in female Sprague–Dawley rats differs from that produced by 17β-estradiol treatment

2009 ◽  
Vol 202 (1) ◽  
pp. 141-152 ◽  
Author(s):  
Rohit Singhal ◽  
Kartik Shankar ◽  
Thomas M Badger ◽  
Martin J Ronis
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Cholesterol Metabolism ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Hepatic Gene Expression ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Wet Weight ◽  
17Β Estradiol

Although soy foods have been recognized as an excellent source of protein, there have been recent concerns regarding potential adverse effects of isoflavone phytochemicals found in soy products, which are known to bind and activate estrogen receptors. Here, we used global hepatic gene expression profiles in ovariectomized female Sprague–Dawley rats treated with 17β-estradiol (E2) or fed with soy protein isolate (SPI) as a means of estimating potential estrogenicity of SPI. Female Sprague–Dawley rats were fed AIN-93G diets containing casein (CAS) or SPI starting at postnatal day (PND) 30. Rats were ovariectomized on PND 50 and infused with E2 or vehicle in osmotic pumps for 14 d. Microarray analysis was performed on liver using Affymetrix GeneChip Rat 230 2.0. Serum E2 levels were within normal ranges for the rat and SPI feeding did not increase uterine wet weight in the absence or presence of E2. SPI feeding altered (P<0.05, ≥±1.5-fold) the expression of 82 genes, while E2 treatment altered 892 genes. Moreover, only 4% of E2-affected genes were also modulated by SPI, including some whose expression was reversed by SPI feeding. The interaction between E2 and SPI uniquely modulated the expression profile of 225 genes including the reduction of those involved in fatty acid biosynthesis or glucocorticoid signaling and an induction of those involved in cholesterol metabolism. The different hepatic gene signatures produced by SPI feeding compared with E2 and the lack of increase in uterine wet weight in rats fed with SPI suggest that SPI is not estrogenic in these tissues.

scholarly journals Maternal Consumption of a Low-Isoflavone Soy Protein Isolate Diet Accelerates Chemically Induced Hepatic Carcinogenesis in Male Rat Offspring

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 571
Author(s):  
Jihye Choi ◽  
Sae Bom Won ◽  
Young Hye Kwon
Keyword(s):  
Soy Protein ◽  
Cholesterol Metabolism ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Protein Source ◽  
Male Rat ◽  
Chow Diet ◽  
Rat Offspring ◽  
Chemically Induced ◽  
Maternal Consumption

It has been reported that maternal nutrition determines the offspring’s susceptibility to chronic diseases including cancer. Here, we investigated the effects of maternal diets differing in protein source on diethylnitrosamine (DEN)-induced hepatocarcinogenesis in adult rat offspring. Dams were fed a casein (CAS) diet or a low-isoflavone soy protein isolate (SPI) diet for two weeks before mating and throughout pregnancy and lactation. Offspring were weaned to and fed a chow diet throughout the study. From four weeks of age, hepatocellular carcinomas (HCC) were induced by intraperitoneal injection of DEN once a week for 14 weeks. The SPI/DEN group exhibited higher mortality rate, tumor multiplicity, and HCC incidence compared with the CAS/DEN group. Accordingly, altered cholesterol metabolism and increases in liver damage and angiogenesis were observed in the SPI/DEN group. The SPI/DEN group had a significant induction of the nuclear factor-κB-mediated anti-apoptotic pathway, as measured by increased phosphorylation of IκB kinase β, which may lead to the survival of precancerous hepatocytes. In conclusion, maternal consumption of a low-isoflavone soy protein isolate diet accelerated chemically induced hepatocarcinogenesis in male rat offspring in the present study, suggesting that maternal dietary protein source may be involved in DEN-induced hepatocarcinogenesis in adult offspring.

scholarly journals Effects of Short and Long-Term Soy Protein Isolate Intake on Hepatic Cytochrome P450 Expression in Obese Zucker Rats

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1225-1225
Author(s):  
Melisa Kozaczek ◽  
Walter Bottje ◽  
Reza Hakkak
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Global Gene Expression ◽  
Protein Isolate ◽  
Zucker Rats ◽  
Z Score ◽  
Short And Long Term ◽  
Obese Zucker Rats

Abstract Objectives To determine the effects feeding for 8 (short-term) and 16 weeks (long-term) soy protein isolate on hepatic CYP gene expression. Methods 7-weeks old rats were randomly assigned to either a casein (CAS) or a soy protein isolate (SPI) diet. They were provided the diets ad libitum for 8 and 16 weeks. Rats were euthanized and livers were stored at − 80°C. RNA was extracted from liver samples, and sequenced to obtain transcriptomic data (RNAseq). Ingenuity Pathway Analysis software (IPA, Qiagen, CA) was used in the analysis of global gene expression data. This analysis includes predictions of activation or inhibition of molecules or upstream regulators and functions based on a generated z-score and p-value of overlap (P = 0.05). Z-scores were consider significant when &gt; 2 (activation) and &lt; −2 (inhibition). Results Comparing short- vs long-term feeding revealed an increase in the number of down-regulated CYP genes from only 3 at 8 weeks of SPI diet to 10 at 16 weeks of same diet (P &lt; 0.05). In contrast, upregulated CYP gene numbers showed a small increase in long-term SPI diet compared to short-term, from 14 genes at 8 weeks to 17 genes at 16 weeks (P &lt; 0.05). In addition, we present a predicted activation of the transcription factor Aryl hydrocarbon receptor (AHR, activation z-score = 2.146, P = 4.20E-11), linked to the subsequent activation or up-regulation of various CYPs genes, indirectly leading to the activation and inhibition of two main metabolic functions under SPI feeding: conversion of lipid (lipid metabolism) –predicted to be activated (z-score = 2.089, P = 2.77E-08), and recruitment of phagocytes (inflammatory response) –predicted to be inhibited (z-score = −2.311, P = 2.10E-05). Conclusions Through global gene expression analysis we showed that gene expression of drug-metabolizing cytochrome P450 genes was modified in genetically obese Zucker rats after being fed a soy-based diet for short- and long-term, and that this change could have an important role in attenuation of liver steatosis. Further research is needed to corroborate these results. Funding Sources This study was supported in part by the College of Medicine's University Medical Group (RH) and the Arkansas Biosciences Institute (WB, RH).

Short Term Effects on Bone Quality Associated with Consumption of Soy Protein Isolate and Other Dietary Protein Sources in Rapidly Growing Female Rats

2008 ◽  
Vol 233 (11) ◽  
pp. 1348-1358 ◽  
Author(s):  
Jin-Ran Chen ◽  
Rohit Singhal ◽  
Oxana P. Lazarenko ◽  
Xiaoli Liu ◽  
William R. Hogue ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Quality ◽  
Soy Protein ◽  
Dietary Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Female Rats ◽  
Protein Sources ◽  
Positive Effects ◽  
Ovx Rats

Beneficial effects of soy protein consumption on bone quality have been reported. The effects of other dietary protein sources such as whey protein hydrolysate (WPH) and rice protein isolate (RPI) on bone growth have been less well examined. The current study compared effects of feeding soy protein isolate (SPI), WPH and RPI for 14 d on tibial bone mineral density (BMD) and bone mineral content (BMC) in intact and ovariectomized (OVX) rapidly growing female rats relative to animals fed casein (CAS). The effects of estrogenic status on responses to SPI were also explored. Tibial peripheral quantitative computerized tomography (pQCT) showed all three protein sources had positive effects on either BMD or BMC relative to CAS ( P < 0.05), but SPI had greater effects in both intact and OVX female rats. SPI and E2 had positive effects on BMD and BMC in OVX rats ( P < 0.05). However, trabecular BMD was lower in a SPI + E2 group compared to a CAS + E2 group. In OVX rats, SPI increased serum bone formation markers, and serum from SPI-fed rats stimulated osteoblastogenesis in ex vivo. SPI also suppressed the bone resorption marker RatLaps ( P < 0.05). Both SPI and E2 increased alkaline phosphatase gene expression in bone, but only SPI decreased receptor activator of nuclear factor-κB ligand (RANKL) and estrogen receptor gene expression ( P < 0.05). These data suggest beneficial bone effects of a soy diet in rapidly growing animals and the potential for early soy consumption to increase peak bone mass.

scholarly journals Uterine physiological responses and global gene expression in ovariectomized (OVX) rats treated with soy protein isolate (SPI) or 17‐βestradiol

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Martin J Ronis ◽  
Michael Blackburn ◽  
Kartik Shankar ◽  
Horatio Gomez-Acevedo ◽  
Rohit Singhal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Physiological Responses ◽  
Soy Protein Isolate ◽  
Global Gene Expression ◽  
Protein Isolate ◽  
Ovx Rats
Sign in / Sign up

Export Citation Format

Share Document