Effect of estrogen/progesterone hormone replacement therapy on natural killer cell cytotoxicity and immunoregulatory cytokine release by peripheral blood mononuclear cells of postmenopausal women

2006 ◽  
Vol 69 (1) ◽  
pp. 65-75 ◽  
Author(s):  
Urszula Stopińska-Głuszak ◽  
Jacek Waligóra ◽  
Tomasz Grzela ◽  
Michał Głuszak ◽  
Jarosław Jóźwiak ◽  
...  
Keyword(s):  
Hormone Replacement Therapy ◽  
Mononuclear Cells ◽  
Hormone Replacement ◽  
Killer Cell ◽  
Cell Cytotoxicity ◽  
Cytokine Release ◽  
Peripheral Blood Mononuclear ◽  
Natural Killer Cell Cytotoxicity ◽  
Immunoregulatory Cytokine ◽  
Blood Mononuclear Cells

Natural Killer Cell Cytotoxicity: A Methods Analysis of Versus Flow Cytometry Chromium Release

2003 ◽  
Vol 5 (2) ◽  
pp. 142-152 ◽  
Author(s):  
Sandra Adams Motzer ◽  
Joyce Tsuji ◽  
Vicky Hertig ◽  
Sandra K. Johnston ◽  
James Scanlan
Keyword(s):  
Flow Cytometry ◽  
Natural Killer ◽  
Mononuclear Cells ◽  
Nk Cell ◽  
Killer Cell ◽  
Cytotoxicity Assay ◽  
Cell Cytotoxicity ◽  
Peripheral Blood Mononuclear ◽  
Natural Killer Cell Cytotoxicity ◽  
Nk Cell Cytotoxicity

The purpose of this study is to describe design considerations for the use of flow cytometry (FC) com-pared to51chromium (51Cr)-release assays utilizing cryopreserved peripheral blood mononuclear cells (PBMCs) to detect natural killer (NK) cell cytotoxicity. Subjects were 10 healthy women aged 18 to 39 years. Intra-assay variability between methods differed only at the lowest effector-target ratios evaluated. Interassay variability was wide but did not differ between methods. The relationship of lytic unit-10 between methods was strongly positive. Cytotoxicity detected by51Cr release was higher than that detected by FC for all 10 subjects. Cost was comparable. How-ever, had more assays been performed, technician time would have been greater with flow cytometry. More whole blood was needed to perform the flow cytometry cytotoxicity assay than51Cr-release cytotoxicity assay. The authors found no compelling reason to adopt NK cell cytotoxicity by flow cytometry over51Cr release.

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