Determination of ABO genotypes by real-time PCR using allele-specific primers

2012 ◽  
Vol 14 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Tomonori Muro ◽  
Junko Fujihara ◽  
Shinji Imamura ◽  
Hiroaki Nakamura ◽  
Kaori Kimura-Kataoka ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Primers ◽  
Allele Specific ◽  
Abo Genotypes

scholarly journals Single-copy detection of somatic variants from solid and liquid biopsy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana-Luisa Silva ◽  
Paulina Klaudyna Powalowska ◽  
Magdalena Stolarek ◽  
Eleanor Ruth Gray ◽  
Rebecca Natalie Palmer ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Real Time Pcr ◽  
Clinical Decision Making ◽  
High Sensitivity ◽  
Clinical Decision ◽  
Single Copy ◽  
Turnaround Time ◽  
Copy Detection ◽  
Allele Specific

AbstractAccurate detection of somatic variants, against a background of wild-type molecules, is essential for clinical decision making in oncology. Existing approaches, such as allele-specific real-time PCR, are typically limited to a single target gene and lack sensitivity. Alternatively, next-generation sequencing methods suffer from slow turnaround time, high costs, and are complex to implement, typically limiting them to single-site use. Here, we report a method, which we term Allele-Specific PYrophosphorolysis Reaction (ASPYRE), for high sensitivity detection of panels of somatic variants. ASPYRE has a simple workflow and is compatible with standard molecular biology reagents and real-time PCR instruments. We show that ASPYRE has single molecule sensitivity and is tolerant of DNA extracted from plasma and formalin fixed paraffin embedded (FFPE) samples. We also demonstrate two multiplex panels, including one for detection of 47 EGFR variants. ASPYRE presents an effective and accessible method that simplifies highly sensitive and multiplexed detection of somatic variants.

Real-Time PCR Determination of Escherichia coli Genomic DNA Contamination in Plasmid Preparations

2002 ◽  
Vol 301 (1) ◽  
pp. 151-153 ◽  
Author(s):  
Adrián Vilalta ◽  
Vanessa Whitlow ◽  
Terrie Martin
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Real Time Pcr ◽  
Genomic Dna ◽  
Dna Contamination

Multiplex real-time PCR for the detection of insect DNA and determination of contents of Tenebrio molitor, Locusta migratoria and Achaeta domestica in food

2019 ◽  
Vol 245 (3) ◽  
pp. 559-567 ◽  
Author(s):  
René Köppel ◽  
Rafael Schum ◽  
Michael Habermacher ◽  
Cindy Sester ◽  
Lucia Eugeni Piller ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Locusta Migratoria ◽  
Tenebrio Molitor

scholarly journals Real-Time PCR as a Versatile Tool for Investigating the Susceptibility of Human Herpesvirus 6 to Antiviral Agents

2003 ◽  
Vol 47 (9) ◽  
pp. 3021-3024 ◽  
Author(s):  
Muriel Macé ◽  
Chaysavanh Manichanh ◽  
Pascale Bonnafous ◽  
Stéphanie Précigout ◽  
David Boutolleau ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Agents ◽  
Antiviral Drug ◽  
Human Herpesvirus ◽  
Drug Susceptibility ◽  
Human Herpesvirus 6 ◽  
Versatile Tool ◽  
Herpesvirus 6

ABSTRACT A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.

Determination of Helicobacter pylori cagA, vacA genotypes with real-time PCR melting curve analysis

2001 ◽  
Vol 95 (1-6) ◽  
pp. 369-377 ◽  
Author(s):  
Agnes Ruzsovics ◽  
Bela Molnar ◽  
Zsuzsa Unger ◽  
Zsolt Tulassay ◽  
Laszlo Pronai
Keyword(s):  
Helicobacter Pylori ◽  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis

scholarly journals A one-step method for quantitative determination of uracil in DNA by real-time PCR

2010 ◽  
Vol 38 (21) ◽  
pp. e196-e196 ◽  
Author(s):  
András Horváth ◽  
Beáta G. Vértessy
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Step Method ◽  
One Step
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