A real-time quantitative RT-PCR assay for monitoring DEK-CAN fusion transcripts arising from translocation t(6;9) in acute myeloid leukemia

2004 ◽  
Vol 28 (11) ◽  
pp. 1213-1215 ◽  
Author(s):  
Mette Østergaard ◽  
Jesper Stentoft ◽  
Peter Hokland
Keyword(s):  
Acute Myeloid Leukemia ◽  
Real Time ◽  
Myeloid Leukemia ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Fusion Transcripts ◽  
Acute Myeloid

Development of a real-time RT-PCR assay for the quantification of the most frequentMLL/AF9fusion types resulting from translocation t(9;11)(p22;q23) in acute myeloid leukemia

2003 ◽  
Vol 38 (3) ◽  
pp. 274-280 ◽  
Author(s):  
Claudia Scholl ◽  
Heike Breitinger ◽  
Richard F. Schlenk ◽  
Hartmut Döhner ◽  
Stefan Fröhling ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Real Time ◽  
Myeloid Leukemia ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Acute Myeloid

scholarly journals Design and Feasibility of a Novel, Rapid, and Simple Fluorescence 26-Plex RT-PCR Assay for Simultaneous Detection of 24 Fusion Transcripts in Adult Acute Myeloid Leukemia

2013 ◽  
Vol 15 (2) ◽  
pp. 186-195 ◽  
Author(s):  
Marie-Pierre Laforêt ◽  
Pascal Turlure ◽  
Eric Lippert ◽  
Pascale Cornillet-Lefebvre ◽  
Arnaud Pigneux ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Fusion Transcripts ◽  
Acute Myeloid

scholarly journals BAALC and ERG Expression in Egyptian Patients with Acute Myeloid Leukemia, Relation to Survival and Response to Treatment

2016 ◽  
Vol 4 (2) ◽  
pp. 264-270 ◽  
Author(s):  
Aml Soliman ◽  
Asmaa Abdel Aal ◽  
Reham Afify ◽  
Noha Ibrahim
Keyword(s):  
Polymerase Chain Reaction ◽  
Acute Myeloid Leukemia ◽  
Reverse Transcription ◽  
Myeloid Leukemia ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain ◽  
Age And Sex ◽  
Acute Myeloid

AIM: Aim was to detect Brain and Acute Leukemia, Cytoplasmic (BAALC) and ETS-related gene (ERG) expression in patients with acute myeloid leukemia (AML) as well as to study their biologic and prognostic impact on the disease outcome and survival.PATIENTS AND METHODS: The current study was carried out on 44 patients with denovo acute myeloid leukemia, as well as 44 age and sex matched controls. The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was performed for estimation of BAALC and ERG expression.RESULTS: The current study was carried out on 44 patients with denovo acute myeloid leukemia, as well as 44 age and sex matched controls. The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was performed for estimation of BAALC and ERG expression. BAALC was expressed in 36 (81.82%) of AML cases versus 10 (22.72%) of the control group which was highly statistically significant (P < 0.001). While ERG was positive in 39(88.64%) of cases and 8(18.18 %) of controls and that was also highly statistically significant (P < 0.001).CONCLUSION: Further researches still needed to clarify the role of BAALC and ERG in the pathogenesis of leukemia and their importance as targets for treatment of AML.

scholarly journals Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis

2004 ◽  
Vol 6 (1) ◽  
pp. 22-27 ◽  
Author(s):  
ShriHari S. Kadkol ◽  
Annette Bruno ◽  
Carol Dodge ◽  
Valerie Lindgren ◽  
Farhad Ravandi
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Comprehensive Analysis ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Fusion Transcripts ◽  
Acute Myeloid

C3aR1 Contributed to Acute Myeloid Leukemia Acquired Resistance to ABT-737 and Involved In the Process of AML

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 1435-1435 ◽  
Author(s):  
Shaoyan Hu ◽  
Saied Mirshahidi ◽  
Chong-Lei Bi ◽  
Wee-Joo Chng ◽  
Heng-Wei Hsu ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Real Time ◽  
Cell Lines ◽  
Myeloid Leukemia ◽  
Acquired Resistance ◽  
Resistant Cell ◽  
Rt Pcr ◽  
Resistant Lines ◽  
Parental Lines ◽  
Acute Myeloid

Abstract Abstract 1435 Abstract ABT-737 is a small molecule antagonist of BCL-2, BCL-XL, and BCL-w currently under evaluation in clinical trials in the oral form of ABT-263 (Navitoclax). Acquired resistance to BCL-2 inhibitors will inevitably emerge. Published pre-clinical data showed increased levels of BFL-1 and/or MCL-1 proteins in lymphoma, and concurrent up-regulation of BCL-XL and BCL2A1 in chronic lymphocytic leukemia, which are not targeted by ABT-737. To investigate potential mechanisms of resistance to BCL-2 inhibitors in acute myeloid leukemia, we developed resistant cell lines by long-term culture of HL-60 and MV4-11 cells with ABT-737, designated as HL-60R and MV4-11R. Parental lines, HL-60 and MV4-11 which were highly sensitive to ABT-737 with IC50 values of 30 nM and 90nM respectively, whereas those for HL-60R and MV4-11R were 10μM and 4.7μM respectively. Annexin V binding assay revealed that both resistant lines were resistant to ABT-737 induced apoptosis as compared to the parental HL-60 and MV4-11 cells. To explore the common pathway mediating acquired resistance to ABT-737, genes differentially expressed 2-fold or greater between parental and resistant lines on HL-60 and MV4-11 were studied. Interestingly, significant changes for BCL-2 family members were not found, suggesting that non-BCL2 family genes could play important roles in mediating the drug resistance to ABT-737. Among them, C3aR1 [Complement component 3a receptor 1] was significantly over-expressed in both resistant cell lines when compared to parental lines and verified with real-time RT-PCR. C3aR1 inhibitor, SB 290157, showed preferential cytotoxicity to both HL-60R and MV4-11R in a dose-dependent way and spared parental cells during 48 hrs in vitro MTT assay. Our findings suggest C3aR1 plays a key role in the resistance phenotype to Bcl-2 inhibitor and blocking C3aR1 revert the resistance. C3aR1 encodes a G-protein coupled seven trans-membrane receptor for C3a, an important inflammatory mediator. The C3a-C3aR axis modulated SDF-1–CXCR4 axis-dependent responses and regulated the homing of hematopoietic stem/progenitor cells into bone marrow. C3aR1 over expression has been associated with FLT3 and D835/I836 mutation cytogenetically normal acute myeloid leukemia, renal cancer and melanoma. We further investigate the role of C3aR1 in 138 cases of AML including 50 cases in initial diagnosis, 68 cases in remission and 20 cases in relapse with real-time RT-PCR. The result showed that C3aR1 expression is highest in initial stage and lowest in remission (p=0.006), there was no significant difference between initial and relapse stages (p=0.384). [Table 1] For newly diagnosed AML patients, high C3aR1 is statistically associated with younger age [median: 34 vs 43 years old], higher presenting WBC [median: 39K vs 27K], higher marrow % blasts [70 vs 55%], but not related to efficacy of first induction treatment, FAB classification or conventional chromosome risk groups.Table 1C3aR1 expression in AML clinical casesAML casesAge (year)C3AR1malefemalemedianrangeM-estimatorrangepInitial2822367-79199.777.49-2514.6P1 = 0.006Remission37313412-7092.2523.45-2286P2 = 0.046Relapse11944.513-54142.7512.4-2372.04P3 = 0.384Note: M-estimator was used for evaluating the differential expression of AML samples and the value was amplified by 104p1: initial vs remission; p2: relapse vs remission; p3 initial vs relapse The biological significant of C3a-C3aR axis in AML and resistance phenotype is intriguing and may suggest a novel mechanism of evading the apoptotic regulation by Bcl-2 gene family as suggested by our current study. Ongoing studies will further elucidate relationship of C3a-C3aR axis in AML. Disclosures: No relevant conflicts of interest to declare.

Rapid identification of CBFB-MYH11–positive acute myeloid leukemia (AML) cases by one single MYH11 real-time RT-PCR

Blood ◽  
2003 ◽  
Vol 101 (12) ◽  
pp. 5085-5086 ◽  
Author(s):  
Bert A. van der Reijden ◽  
Marion Massop ◽  
Evelyn Tönnissen ◽  
Louis van de Locht ◽  
Petra Muus ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Real Time ◽  
Myeloid Leukemia ◽  
Rapid Identification ◽  
Rt Pcr ◽  
Acute Myeloid

scholarly journals Quantification of CBFβ/MYH11 fusion transcript by Real Time RT-PCR in patients with INV(16) acute myeloid leukemia

Leukemia ◽  
2001 ◽  
Vol 15 (7) ◽  
pp. 1072-1080 ◽  
Author(s):  
G Marcucci ◽  
MA Caligiuri ◽  
H Döhner ◽  
KJ Archer ◽  
RF Schlenk ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Real Time ◽  
Myeloid Leukemia ◽  
Fusion Transcript ◽  
Rt Pcr ◽  
Acute Myeloid
Sign in / Sign up

Export Citation Format

Share Document