Clinical monitoring and misoprostol for early embryo abortion based on embedded computer system

2021 ◽  
Vol 81 ◽  
pp. 103765
Author(s):  
Junmei He ◽  
Qin Zhao ◽  
Cong Chen
Keyword(s):  
Computer System ◽  
Early Embryo ◽  
Clinical Monitoring ◽  
Embryo Abortion ◽  
Embedded Computer

scholarly journals Aneuploid abortion correlates positively with MAD1 overexpression and miR-125b down-regulation

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Juan Zhao ◽  
Hui Li ◽  
Guangxin Chen ◽  
Lijun Du ◽  
Peiyan Xu ◽  
...  
Keyword(s):  
Chromosome Segregation ◽  
Spindle Assembly Checkpoint ◽  
Mitotic Checkpoint ◽  
Early Embryo ◽  
Vital Role ◽  
Control Group ◽  
Embryo Abortion ◽  
Protein Levels ◽  
Mitotic Checkpoint Complex

Abstract Background Aneuploidy is the most frequent cause of early-embryo abortion. Any defect in chromosome segregation would fail to satisfy the spindle assembly checkpoint (SAC) during mitosis, halting metaphase and causing aneuploidy. The mitotic checkpoint complex (MCC), comprising MAD1, MAD2, Cdc20, BUBR1 and BUB3, plays a vital role in SAC activation. Studies have confirmed that overexpression of MAD2 and BUBR1 can facilitate correct chromosome segregation and embryo stability. Research also proves that miR-125b negatively regulates MAD1 expression by binding to its 3′UTR. However, miR-125b, Mad1 and Bub3 gene expression in aneuploid embryos of spontaneous abortion has not been reported to date. Methods In this study, embryonic villi from miscarried pregnancies were collected and divided into two groups (aneuploidy and euploidy) based on High-throughput ligation-dependent probe amplification (HLPA) and Fluorescence in situ hybridization (FISH) analyses. RNA levels of miR-125b, MAD1 and BUB3 were detected by Quantitative real-time PCR (qRT-PCR); protein levels of MAD1 and BUB3 were analysed by Western blotting. Results statistical analysis (p < 0.05) showed that miR-125b and BUB3 were significantly down-regulated in the aneuploidy group compared to the control group and that MAD1 was significantly up-regulated. Additionally, the MAD1 protein level was significantly higher in aneuploidy abortion villus, but BUB3 protein was only mildly increased. Correlation analysis revealed that expression of MAD1 correlated negatively with miR-125b. Conclusion These results suggest that aneuploid abortion correlates positively with MAD1 overexpression, which might be caused by insufficient levels of miR-125b. Taken together, our findings first confirmed the negative regulatory mode between MAD1 and miR-125b, providing a basis for further mechanism researches in aneuploid abortion.

Research on Reconfigurable High-Speed Serial Bus Used in High-Reliability Embedded Computer

2010 ◽  
Vol 20-23 ◽  
pp. 774-778
Author(s):  
Rui Ding ◽  
Yong Qin Hu ◽  
Wei Gong Zhang ◽  
Bo Yang
Keyword(s):  
Computer System ◽  
High Speed ◽  
Communication Protocol ◽  
High Reliability ◽  
Dynamic Reconfiguration ◽  
System A ◽  
Embedded Computer ◽  
Key Points ◽  
Application Scope

The characteristics and limitations of the buses, which are widely used nowadays, are analyzed in this paper. Because these buses don’t adapt to the high-reliability embedded computer system, a novel bus is proposed which is characterized by its high-reliability. And its capacity is reached through its unique datum’s dynamic reconfiguration mechanism. The basic architecture and communication protocol are presented in this paper. And then the key points during realization of this bus are discussed. Finally, the probably application scope and prospects are indicated.

Embedded Computer System Requirements Workshop

Computer ◽  
1985 ◽  
Vol 18 (4) ◽  
pp. 67-70 ◽  
Author(s):  
White ◽  
Lavi
Keyword(s):  
Computer System ◽  
Embedded Computer ◽  
System Requirements

Crossability Barriers in Interspecific Hybridization of Ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi] with Other Vigna Species

2021 ◽  
Author(s):  
Neelam Bhardwaj ◽  
Tanuja Kapoor ◽  
Parveen Sharma
Keyword(s):  
Growth Habit ◽  
Growth Period ◽  
Early Embryo ◽  
Grain Legume ◽  
Cropping Pattern ◽  
Storage Pests ◽  
Embryo Abortion ◽  
Vigna Species ◽  
Vigna Umbellata ◽  
Specific Hybridization

Background: Ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi] is a multipurpose grain legume of Mid-Himalayan region mainly cultivated for food, fodder, green manure and has emerged as a good alternative to other pulse crops such as blackgram and greengram which do not flourish in this region due to their susceptibility to cold temperature stress. It is well reported that the nutritional value of ricebean is higher as compared to many other legumes of the Vigna family and has some superior qualities greater than greengram, blackgram and cowpea. It is also resistance to drought, diseases and pests specially the storage pests during growth period and possesses high percentage of seed viability. Despite having all the favourable traits, it is not much popular among the farmers due to the late maturity and indeterminate growth habit. Instead, farmers prefer other crops which fit easily into their cropping pattern and are easy to harvest. A little genetic improvement with respect to maturity and growth habit can revive its cultivation and show great results in its production as a valuable crop. Thus, the present investigation was formulated to introgress desired traits from mash and adzukibean into otherwise high yielding ricebean genotypes using inter-specific hybridization. Methods: The present investigation involves the inter-specific hybridization among three Vigna species viz, ricebean (Vigna umbellata), blackgram (Vigna mungo) and adzukibean (Vigna angularis). In the year 2017, six genotypes of ricebean (RBHP-36, RBHP-38, RBHP-43, RBHP-61, RBHP-107 and RBHP-108) were crossed with two genotypes of blackgram (HimMash-1 and Palampur-93) and one genotype of adzukibean (HPU-51) in glasshouse conditions. Result: The study revealed that successful crosses were possible only between ricebean and blackgram. All the Inter-specific crosses showed very low pod set percentage ranging from 0 -4% and F1 germination percentage ranging from 20-42%. Pod set percentage and pods harvested varied with combinations of two parental cultivars of each species for most of the inter-specific hybrids. The successful pod set was observed in 16 out of 36 inter-specific crosses. Highest crossability was observed in blackgram and ricebean crosses. Crossing of adzukibean with ricebean showed poor or no pod set among the entire cross combinations which are attributed to early embryo abortion and degeneration during embryogenesis.

scholarly journals Mating System in a Native Norway Spruce (Picea abies [L.] KARST.) Stand-Relatedness and Effective Pollen Population Size Show an Association with the Germination Percentage of Single Tree Progenies

Diversity ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 266
Author(s):  
Oliver Caré ◽  
Oliver Gailing ◽  
Markus Müller ◽  
Konstantin V. Krutovsky ◽  
Ludger Leinemann
Keyword(s):  
Gene Flow ◽  
Population Size ◽  
Norway Spruce ◽  
High Elevation ◽  
Germination Percentage ◽  
Early Embryo ◽  
Genetic Composition ◽  
Embryo Abortion ◽  
Family Structures ◽  
Single Tree

Norway spruce differs little in neutral genetic markers among populations and provenances often reported, but in terms of putative adaptive traits and their candidate genes, some clear differences have been observed. This has previously been shown for crown morphotypes. Stands with mostly narrow crown shapes are adapted to high elevation conditions, but these stands are scattered, and the forest area is often occupied by planted stands with predominantly broad crowned morphotypes. This raises questions on whether this differentiation can remain despite gene flow, and on the level of gene flow between natural and planted stands growing in close neighbourhood. The locally adapted stands are a valuable seed source, the progeny of which is expected to have high genetic quality and germination ability. The presented case study is useful for spruce plantation by demonstrating evaluation of these expectations. Immigrant pollen and seeds from planted trees could be maladaptive and may alter the genetic composition of the progeny. This motivated us to study single tree progenies in a locally adapted stand with narrow crowned trees in a partial mast year at nuclear genomic simple sequence repeat (SSR) markers. Spruce is a typical open-pollinated conifer tree species with very low selfing rates, which were also observed in our study (s = 0.3–2.1%) and could be explained by efficient cross-pollination and postzygotic early embryo abortion, common in conifers. The estimated high amount of immigrant pollen found in the pooled seed lot (70.2–91.5%) is likely to influence the genetic composition of the seedlings. Notably, for individual mother trees located in the centre of the stand, up to 50% of the pollen was characterised as local. Seeds from these trees are therefore considered to retain most of the adaptive variance of the stand. Germination percentage varied greatly between half-sib families (3.6–61.9%) and was negatively correlated with relatedness and positively with effective pollen population size of the respective families. As pollen mostly originated from outside the stand and no family structures in the stand itself were found, germination differences can likely be explained by diversity differences in the individual pollen cloud.

scholarly journals AtNSE1 and AtNSE3 are required for embryo pattern formation and maintenance of cell viability during Arabidopsis embryogenesis

2019 ◽  
Vol 70 (21) ◽  
pp. 6229-6244
Author(s):  
Gang Li ◽  
Wenxuan Zou ◽  
Liufang Jian ◽  
Jie Qian ◽  
Jie Zhao
Keyword(s):  
Cell Death ◽  
Cell Viability ◽  
Cell Fate ◽  
Embryo Development ◽  
Higher Plants ◽  
Early Embryo ◽  
Embryo Cell ◽  
Early Embryogenesis ◽  
Embryo Abortion ◽  
Cell Fate Decisions

Abstract Embryogenesis is an essential process during seed development in higher plants. It has previously been shown that mutation of the Arabidopsis non-SMC element genes AtNSE1 or AtNSE3 leads to early embryo abortion, and their proteins can interact with each other directly. However, the crucial regions of these proteins in this interaction and how the proteins are cytologically involved in Arabidopsis embryo development are unknown. In this study, we found that the C-terminal including the Ring-like motif of AtNSE1 can interact with the N-terminal of AtNSE3, and only the Ring-like motif is essential for binding with three α motifs of AtNSE2 (homologous to AtMMS21). Using genetic assays and by analysing molecular markers of cell fate decisions (STM, WOX5, and WOX8) in mutant nse1 and nse3 embryos, we found that AtNSE1 and AtNSE3 work non-redundantly in early embryo development, and that differentiation of the apical meristem and the hypophysis fails in the mutants, which have disrupted auxin transportation and responses. However, the upper cells of the suspensor in the mutants seem to have proper embryo cell identity. Cytological examination showed that cell death occurred from the early embryo stage, and that vacuolar programmed cell death and necrosis in the nse1 and nse3 mutant embryos led to ovule abortion. Thus, AtNSE1 and AtNSE3 are essential for maintaining cell viability and growth during early embryogenesis. Our results improve our understanding of the functions of SMC5/6 complex in early embryogenesis in Arabidopsis.

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