P045. Local blue laser irradiation improves tissue circulation by photochemical release of nitric oxide from nitrosyl hemoglobin

Nitric Oxide ◽  
2006 ◽  
Vol 14 (4) ◽  
pp. 32
Author(s):  
Rainer Mittermayr ◽  
Anatoly Osipov ◽  
Christina Piskernik ◽  
Susanne Haindl ◽  
Peter Dungel ◽  
...  
2016 ◽  
Vol 96 (5) ◽  
pp. 796-807
Author(s):  
Yi-ping Chen ◽  
Qiang Liu ◽  
Dong Chen

To investigate the mechanism by which laser irradiation enhances the chilling tolerance of wheat seedlings, seeds were exposed to different treatments, and biochemical parameters were measured. Compared with the control group, chilling stress (CS) led to an increase in the concentrations of malondialdehyde (MDA) and H2O2, and decreases in the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), catalase (CAT), peroxidase (POD), and nitric oxide synthase (NOS), and the concentrations of nitric oxide (NO) and protein. Treatment with 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), sodium tungstate (ST), and NG-nitro-L-arginine methyl ester (L-NAME) followed by CS resulted in further increases in the concentrations of MDA and H2O2 and further decreases in the other parameters. However, treatment with PTIO, ST, and L-NAME followed by laser irradiation had the opposite effects on these parameters. When the seeds were treated with PTIO, ST, and L-NAME followed by laser and CS, the concentrations of MDA and H2O2 were significantly lower and the other parameters were higher than in the PTIO, ST, and L-NAME plus CS groups. These results suggest that CO2 laser irradiation enhances the chilling tolerance of wheat seedlings by stimulating endogenous NO synthesis.


2010 ◽  
Vol 98 (3) ◽  
pp. 211-215 ◽  
Author(s):  
Toshihiro Kushibiki ◽  
Takako Tajiri ◽  
Yoshihisa Ninomiya ◽  
Kunio Awazu

2008 ◽  
Vol 145 (6) ◽  
pp. 751-754 ◽  
Author(s):  
V. M. Chertok ◽  
A. E. Kotsyuba ◽  
E. V. Bespalova

2006 ◽  
Vol 71 (10) ◽  
pp. 1128-1132 ◽  
Author(s):  
A. N. Osipov ◽  
G. O. Stepanov ◽  
Yu. A. Vladimirov ◽  
A. V. Kozlov ◽  
V. E. Kagan

PeerJ ◽  
2022 ◽  
Vol 9 ◽  
pp. e12684
Author(s):  
Hansheng Li ◽  
Yuqiang Qiu ◽  
Gang Sun ◽  
Wei Ye

Dendrobium officinale Kimura et Migo (D. officinale) has promising lung moisturizing, detoxifying, and immune boosting properties. Light is an important factor influencing functional metabolite synthesis in D. officinale. The mechanisms by which lasers affect plants are different from those of ordinary light sources; lasers can effectively address the shortcomings of ordinary light sources and have significant interactions with plants. Different light treatments (white, blue, blue laser) were applied, and the number of red leaves under blue laser was greater than that under blue and white light. RNA-seq technology was used to analyze differences in D. officinale under different light treatments. The results showed 465, 2,107 and 1,453 differentially expressed genes (DEGs) in LB-B, LB-W and W-B, respectively. GO, KEGG and other analyses of DEGs indicated that D. officinale has multiple blue laser response modes. Among them, the plasma membrane, cutin, suberine and wax biosynthesis, flavone and flavonol biosynthesis, heat shock proteins, etc. play central roles. Physiological and biochemical results verified that blue laser irradiation significantly increases POD, SOD, and PAL activities in D. officinale. The functional metabolite results showed that blue laser had the greatest promoting effect on total flavonoids, polysaccharides, and alkaloids. qPCR verification combined with other results suggested that CRY DASH, SPA1, HY5, and PIF4 in the blue laser signal transduction pathway affect functional metabolite accumulation in D. officinale through positively regulated expression patterns, while CO16 and MYC2 exhibit negatively regulated expression patterns. These findings provide new ideas for the efficient production of metabolites in D. officinale.


2019 ◽  
Vol 7 (2) ◽  
pp. 354-361 ◽  
Author(s):  
Xuejie Zhang ◽  
Shuaichen Si ◽  
Jinbo Yu ◽  
Zijun Wang ◽  
Ronghuan Zhang ◽  
...  

The luminous efficacy and durability of white LDs are obviously enhanced though employing dual-functional sapphire plate.


2020 ◽  
Vol 10 (3) ◽  
pp. 1189 ◽  
Author(s):  
Mohammed Hasan ◽  
Marlia Mohd Hanafiah ◽  
Ziad Aeyad Taha ◽  
Intsar H. H. AlHilfy ◽  
Mohd Nizam Mohd Said

This study aims to compare the effects of different laser wavelengths, exposure times, and low-power-intensity laser irradiation on maize seeds. Seeds were exposed to He–Ne (632.8 nm) red laser, Nd:YAG second-harmonic-generation (532 nm) green laser, and diode (410 nm) blue laser. Four different exposure times (45, 65, 85, and 105 s) with different intensity (2 and 4 mW/cm2), for each laser were tested. Phenology and yield components (plant height, leaf area, number of rows per ear, seed yield, harvest index, yield efficiency, and grain weight) were determined. The experiment was conducted in a randomized complete block design with three replications. Plant height was found comparatively high in blue laser light—211 cm at 85 s. Blue and green laser lights showed significant increases in the number of rows per ear to 39.1 at 85 s and 45 at 65 s, respectively, compared to the control of 36 rows/ear. The order of seed yield was blue (7003.4 kg/ha) > green (6667.8 kg/ha) > red (6568.01 t/ha) based on different exposure times of 85 s, 85 s, and 105 s, respectively, compared to the control of 6.9 kg/ha. The findings indicate the possibility of using blue laser light to manipulate the growth and yield of maize.


1996 ◽  
Vol 270 (3) ◽  
pp. C748-C752 ◽  
Author(s):  
E. Kumura ◽  
T. Yoshimine ◽  
K. I. Iwatsuki ◽  
K. Yamanaka ◽  
S. Tanaka ◽  
...  

We investigated the levels of nitrosyl hemoglobin (HbNO) in rat jugular blood by electron spin resonance (ESR) spectroscopy during and after middle cerebral artery occlusion. The levels of plasma nitric oxide (NO) end products, nitrate plus nitrate, were compared with the levels of HbNO. Small amounts of HbNO were detected in sham-operated rats (n=4) and those subjected to 2 h of occlusion (n=4), whereas nitrite plus nitrate was increased only in the latter (P<0.01; vs.sham). Upon reperfusion after 2 h of occlusion both HbNO and nitrite plus nitrate clearly increased after 15 min (n=4) and 30 min (n=6) reperfusion (P<0.01; vs.occlusion). Administration of superoxide dismutase (5 mg/kg) significantly increased HbNO (P<0.05) but not plasma nitrate plus nitrate (n=5). The increase in HbNO suppressed by administration of NG-nitro-L-arginine methyl ester (20mg/kg; n=4,P<0.01), and this suppression could be reversed by L-arginine (200 mg/kg) (n=4). The present study clearly showed that the L-arginine-NO synthase pathway was activated during reperfusion after focal cerebral ischemia and indicated the involvement of a reaction between NO and superoxide during early reperfusion.


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