Skin temperature contribution to the decrease in withdrawal latency following chronic constriction injury

2020 ◽  
Vol 227 ◽  
pp. 113147
Author(s):  
Lucia Voděrová ◽  
Štěpán Šandera ◽  
Tomáš Drobil ◽  
Barbora Plevová ◽  
Šimon Vaculín
Keyword(s):  
Skin Temperature ◽  
Chronic Constriction Injury ◽  
Withdrawal Latency

Lidocaine activates autophagy of astrocytes and ameliorates chronic constriction injury-induced neuropathic pain

2020 ◽  
Author(s):  
Jiaqi Yuan ◽  
Yue Fei
Keyword(s):  
Cell Proliferation ◽  
Neuropathic Pain ◽  
Chronic Constriction Injury ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Factor ◽  
Experimental Basis ◽  
Withdrawal Threshold ◽  
Withdrawal Latency ◽  
Tnf Α ◽  
Rat Astrocytes

Abstract Lidocaine is a commonly used drug to alleviate neuropathic pain (NP). This work aims to investigate the mechanism of lidocaine in alleviating NP. Chronic constriction injury (CCI) rats were established by surgery to induce NP. We observed the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of rats. Immunofluorescence staining was performed to determine the LC3/glial fibrillary acidic protein (GFAP)-positive cells. Rat astrocytes were treated with lipopolysaccharide (LPS) to induce CCI, and then treated with lidocaine or 3-MA (autophagy inhibitor). CCK-8 was performed to detect cell proliferation. Western blot and enzyme-linked immunosorbent assay were performed to detect the level of protein and inflammatory factor. CCI rats exhibited a decrease of MWT and TWL, which was effectively abolished by lidocaine. Lidocaine enhanced the number of LC3/GFAP-positive cells in CCI rats. Moreover, lidocaine inhibited the expression of GFAP and p62, and enhanced LC3-II/LC3-I expression in the LPS-treated astrocytes. Lidocaine inhibited the level of TNF-α and IL-1β in the LPS-treated astrocytes. The influence conferred by lidocaine was effectively abolished by 3-MA. In conclusion, our work demonstrates that lidocaine activates autophagy of astrocytes and ameliorates CCI-induced NP. Thus, our study provides a further experimental basis for the mechanism of lidocaine to alleviate NP.

scholarly journals Pulsed Radiofrequency Reduced Neuropathic Pain Behavior in Rats Associated with Upregulation of GDNF Expression

2016 ◽  
Vol 19 (2;2) ◽  
pp. 49-58 ◽  
Author(s):  
Luo Fang
Keyword(s):  
Chronic Constriction Injury ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pulsed Radiofrequency ◽  
Glia Cell ◽  
Group 14 ◽  
Time Points ◽  
Withdrawal Threshold ◽  
Withdrawal Latency ◽  
The Right ◽  
Ligation Site

Background: Pulsed radiofrequency (PRF) is a novel nondestructive interventional technique for the treatment of neuropathic pain (NP). However, this intervention is still lack of relevant regulation and the mechanism of action is insofar not clear. Historically, most studies have reported that PRF can relieve reduce hyperalgesia in multiple NP animal models by acting on the dorsal root ganglion. However, a few recent studies have shown that PRF can effectively treat hyperalgesia in pain models by a direct application on injured peripheral nerves. Objectives: To observe changes in pain behavior and the pathology of the sciatic nerve (SN) after applying PRF at the ligation site in a chronic constriction injury (CCI) rat model and to investigate the effect of PRF on the expression of glia cell line-derived neurotrophic factor (GDNF) in nervous tissue. Study Design: A randomized, experimental trial. Setting: Experimental Animal Center, Beijing Tiantan Hospital, Capital Medical University. Methods: Thirty-six adult Sprague-Dawley rats were randomly divided into 3 groups: Sham-Sham (SS), CCI-Sham (CS), and CCI-PRF (CP). The right SNs of the rats in the CS and CP groups were ligated to create a CCI model. For the SS group, the right SN was separated without ligation. On the 14th fourteenth day after surgery, PRF treatment was applied at the ligation site of the SN for the rats in the CP group using a 45 V output voltage at 42°C for 3 minutes. The electrode was placed in rats in the SS and CS groups without electricity applied. The hindpaw withdrawal threshold (HWT) and thermal withdrawal latency (TWL) were measured at various time points before and after the treatments in each group. Optical microscopic scores and electron microscopic observation were given to the right SN ligation sites of the rats in each group 14 days after the treatment . Meanwhile, the GDNF expression levels in the ligation site of the SN and in the L4-L6 spinal cord segments were determined for each group by enzyme-linked immunosorbent assay (ELISA). Results: Fourteen days after PRF treatment, the HWT and TWL values in the CP group were significantly increased compared to those of the CS group (P < 0.01). Under the optical microscope, the axonal number, axonal diameter, and myelin sheath thickness in the CP group were significantly increased compared to those of the CS group 14 days after PRF treatment (P < 0.01). Under the electron microscope, the degeneration at the SN ligation site was significantly improved in the CP group compared to the CS group. The GDNF expression levels at the ligation site of the SN and the L4-L6 spinal segments in the CP and CS groups were increased compared to those of the SS group (P < 0.01). In addition, the GDNF expression in the CP group was significantly higher than that in the CS group (P < 0.01). Limitations: GDNF expression was only measured at day 14 after the treatment rather than at various time points during the experiment. Conclusions: The findings suggest that the application of PRF at the impaired SN relieved reduced the CCI-induced NP by through regulating the upregulation of the GDNF expression in the nervous tissues. Key words: Pulsed radiofrequency, chronic constriction injury, sciatic nerve, spinal cord, hind paw withdrawal threshold, thermal withdrawal latency, optical microscopic, electron microscope, glia cell line-derived neurotrophic factor, enzyme-linked immunosorbent assay

scholarly journals Increase in the glutamate transporter 1 and time withdrawal latency following wet cupping therapy in chronic constriction injury in rats

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Hanik Badriyah Hidayati ◽  
Muhammad Hasan Machfoed ◽  
Kuntoro . ◽  
Imam Subadi ◽  
Siti Khaerunnisa ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Chronic Constriction Injury ◽  
Controlled Trial ◽  
Somatosensory System ◽  
Glutamate Transporter ◽  
Cupping Therapy ◽  
Male Rats ◽  
Withdrawal Latency ◽  
Pain Models ◽  
Glutamate Transporter 1

Objective: Neuropathic pain (NP) is a chronic debilitating pain and is caused by disease or lesion of somatosensory system. NP respond worse to the pharmacological drugs leading to this pain still a big problem in medical treatment and furthermore make many patients seek alternative treatment. Wet cupping therapy (WCT) has been widely used to relief both of acute and chronic pain, but the mechanism for reducing pain has not yet been clear. Recent studies have shown that NP is associated with alteration of GLT-1/EAAT2, and WCT has beneficial role to reduce the pain in various pain models. This is the pilot study, no other study has applied WCT in chronic constriction injury (CCI) models, the most commonly employed animal model of NP. Therefore, we investigate the association between WCT and the reducing pain by looking at the increase of GLT-1 and time withdrawal latency (TWL) in rats with CCI. Methodology: The study design was randomized, post-test only, controlled trial with a total of 21 male rats (Rattus Norvegicus) with CCI, aged 4 months, weighing 220 to 250 g, randomly divided into three groups, P1 (sham CCI group), P2 (CCI group), and P3 (CCI group plus WCT). WCT had been applied 2 times/week for 3 weeks to all of the groups in paralumbar region, both left and right side. TWL was recorded to assess pain threshold of the rats by hot plate and the expression of GLT-1 on glial cells in spinal cord were counted. Results: This study revealed that mean ± SD values for P1, P2, and P3 were 7.20 ± 1.30, 2.57 ± 1.27, and 18.20 ± 3.50 respectively. There were significant differences in the TWL between groups P1-P2, P1-P3, and P2-P3 (p = 0.003, p = 0.0001, and p = 0.0001 respectively) and GLT-1 increase was significant between groups P2-P3 (p =  0.009). Conclusion: It can be concluded that wet cupping therapy decreases the pain by increasing the time withdrawal latency and GLT-1 in chronic constriction injury models. We suggest that wet cupping therapy as a promising method to reduce pain in peripheral neuropathic pain models. However, further investigation is still needed to confirm its mechanism of action. Key words: GLT-1/EAAT2; Neuropathic pain; Wet cupping therapy; Chronic constriction injury; CCI, TWL Citation: Hidayati HB, Machfoed MH, Kuntoro, Subadi I, Khaerunnisa S, Widjiati. Increase in the glutamate transporter 1 and time withdrawal latency following wet cupping therapy in chronic constriction injury in rats. Anaesth. pain & intensive care 2021;25(1):48-54. DOI: 10.35975/apic.v25i1.1441 Received: 24 January 2019, Reviewed: 4 January 2019, 14 January 2019, Revised: 24 January 2019, Accepted: 20 May 2019

Skin Temperature Guides Cellulitis Treatment

2012 ◽  
Vol 5 (11) ◽  
pp. 14
Author(s):  
M. ALEXANDER OTTO
Keyword(s):  
Skin Temperature

Hypnotic control of complex skin temperature

1971 ◽  
Author(s):  
Christina Maslach ◽  
Garry Marshall ◽  
Philip Zimbardo
Keyword(s):  
Skin Temperature

Changes in skin temperature with wet dressing therapy

1967 ◽  
Vol 96 (6) ◽  
pp. 708-711 ◽  
Author(s):  
C. A. Quinones
Keyword(s):  
Skin Temperature

Measuring Facial Skin Temperature Changes Caused by Mental Work-Load with Infrared Thermography

2016 ◽  
Vol 136 (11) ◽  
pp. 1581-1585 ◽  
Author(s):  
Tota Mizuno ◽  
Takeru Sakai ◽  
Shunsuke Kawazura ◽  
Hirotoshi Asano ◽  
Kota Akehi ◽  
...  
Keyword(s):  
Skin Temperature ◽  
Infrared Thermography ◽  
Work Load ◽  
Facial Skin ◽  
Mental Work ◽  
Temperature Changes ◽  
Mental Work Load

638-P: Innovative Diagnostic Tool for Assessment of Plantar Skin Temperature to Spot Diabetes-Related Foot Complications

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 638-P
Author(s):  
ARATI A. SHAHADE ◽  
ANUJ A. SHAHADE ◽  
AMBRISH M. SHAHADE ◽  
SAGAR P. WAGH ◽  
SHOUNAK OZARKAR
Keyword(s):  
Skin Temperature ◽  
Diagnostic Tool ◽  
Foot Complications
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