A fully automated screening method for detecting compounds with goitrogenic activity using transgenic zebrafish embryos

2016 ◽  
Vol 64 ◽  
pp. 30
Author(s):  
Stefan Scholz ◽  
Sergio Jarque ◽  
Eva Fetter
Keyword(s):  
Screening Method ◽  
Transgenic Zebrafish ◽  
Zebrafish Embryos ◽  
Automated Screening

scholarly journals An automated screening method for detecting compounds with goitrogenic activity using transgenic zebrafish embryos

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0203087 ◽  
Author(s):  
Sergio Jarque ◽  
Eva Fetter ◽  
Wouter J. Veneman ◽  
Herman P. Spaink ◽  
Ravindra Peravali ◽  
...  
Keyword(s):  
Screening Method ◽  
Transgenic Zebrafish ◽  
Zebrafish Embryos ◽  
Automated Screening

Ultramicro Automated Screening Method for Uric Acid

1967 ◽  
Vol 13 (11) ◽  
pp. 985-993 ◽  
Author(s):  
Ronald H Laessig ◽  
Chester E Underwood ◽  
Barbara J Basteyns
Keyword(s):  
Uric Acid ◽  
Phosphotungstic Acid ◽  
Screening Method ◽  
Sampling Procedure ◽  
Blood Capillary ◽  
Acid Complex ◽  
Blood Uric Acid ◽  
Precision And Accuracy ◽  
Automated Screening

Abstract An automated colorimetric microprocedure, suitable for screening purposes, has been developed for the determination of blood uric acid levels. The method uses 2O-µl. whole-blood (capillary) samples and is based on the AutoAnalyzer measurement of the absorbance of the colored uric acid-phosphotungstic acid complex. The dilution inherent in the sampling procedure necessitated a modification of the existing AutoAnalyzer method to increase the sensitivity. The proposed method is evaluated for precision and accuracy by comparison with the standard AutoAnalyzer macro-method.

Preparation of Transgenic Zebrafish Embryos for Imaging the Developing Retina

2013 ◽  
Vol 2013 (3) ◽  
pp. pdb.prot073536 ◽  
Author(s):  
Patricia Jusuf ◽  
William A. Harris ◽  
Lucia Poggi
Keyword(s):  
Transgenic Zebrafish ◽  
Zebrafish Embryos

scholarly journals Cardioluminescence in transgenic zebrafish embryos: a calcium imaging tool to study drug effects and pathological modeling

2021 ◽  
Author(s):  
Manuel Vicente ◽  
Jussep Salgado-Almario ◽  
Michelle M. Collins ◽  
Antonio Martinez-Sielva ◽  
Masafumi Minoshima ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Light Output ◽  
Drug Effects ◽  
Study Drug ◽  
Transgenic Zebrafish ◽  
Zebrafish Embryos ◽  
Cardiovascular Research ◽  
Imaging Tool ◽  
Adrenergic Blocker

The zebrafish embryo has emerged as an excellent model in cardiovascular research. The existing techniques to monitor Ca2+ in the heart based on fluorescent Ca2+ biosensors are limited due to phototoxicity and photobleaching. To overcome these issues, we have used bioluminescence. We generated a transgenic line expressing GFP-Aequorin in the heart, Tg(cmlc2:GA), and optimized an in vivo aequorin reconstitution protocol to improve the luminescence capacity. This allowed imaging Ca2+ in long duration recordings in embryos of 3 to 5 days post-fertilization. The analogs diacetyl h-coelenterazine and f-coelenterazine enhanced the light output and signal-to-noise ratio from the embryos. With this cardioluminescence model, we monitored the time-averaged Ca2+ levels and beat-to-beat Ca2+ oscillations. Changes in Ca2+ levels were observed by incubation with BayK8644, an L-type Ca2+ channel agonist, the channel blocker nifedipine, and β-adrenergic blocker propranolol. Treatment of zebrafish embryos with terfenadine for 24 hours has been proposed as a model of heart failure. Tg(cmlc2:GA) embryos treated with terfenadine showed a 2:1 atrioventricular block and a decrease in the ventricular Ca2+ levels.

Dynamic analysis of angiogenesis in transgenic zebrafish embryos using a 3D multilayer chip-based technology

2013 ◽  
Author(s):  
Jin Akagi ◽  
Feng Zhu ◽  
Chris J. Hall ◽  
Khashayar Khoshmanesh ◽  
Kourosh Kalantar-Zadeh ◽  
...  
Keyword(s):  
Dynamic Analysis ◽  
Transgenic Zebrafish ◽  
Zebrafish Embryos

scholarly journals Cytotoxic Evaluation and Anti-Angiogenic Effects of Two Furano-Sesquiterpenoids from Commiphora myrrh Resin

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1318 ◽  
Author(s):  
Ali S. Alqahtani ◽  
Fahd A. Nasr ◽  
Omar M. Noman ◽  
Muhammad Farooq ◽  
Tariq Alhawassi ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Umbilical Vein ◽  
Flow Cytometric Analysis ◽  
Ethanolic Extract ◽  
Liver Carcinoma ◽  
Transgenic Zebrafish ◽  
Chloroform Fraction ◽  
Zebrafish Embryos ◽  
Mcf7 Cells ◽  
Angiogenic Activity

Commiphora myrrh resin (Myrrh) has been used in traditional Arabic medicine to treat various inflammatory diseases. Two furano-sesquiterpenoids, 2-methoxyfuranodiene (CM1) and 2-acetoxyfuranodiene (CM2), were isolated from the chloroform fraction of the ethanolic extract of Arabic Commiphora myrrh resin. The cytotoxicity of the compounds was evaluated using human liver carcinoma, breast cancer cells (HepG2 and MCF-7, respectively) and normal human umbilical vein endothelial cells (HUVECs) cell lines. The development toxicity and anti-angiogenic activity of both compounds were also evaluated using zebrafish embryos. Cell survival assays demonstrated that both compounds were highly cytotoxic in HepG2 and MCF7 cells, with IC50 values of 3.6 and 4.4 µM, respectively. Both compounds induced apoptosis and caused cell cycle arrest in treated HepG2 cells, which was observed using flow cytometric analysis. The development toxicity in zebrafish embryos showed the chronic toxicity of both compounds. The toxicity was only seen when the embryos remained exposed to the compounds for more than three days. The compound CM2 showed a significant level of anti-angiogenic activity in transgenic zebrafish embryos at sublethal doses. Thus, we demonstrated the cytotoxic properties of both compounds, suggesting that the molecular mechanism of these compounds should be further assessed.

scholarly journals Lobetyolin Efficiently Promotes Angiogenesis and Neuronal Development in Transgenic Zebrafish

2020 ◽  
Vol 15 (8) ◽  
pp. 1934578X2093717
Author(s):  
Chengniu Wang ◽  
Jie Hui ◽  
Xinhui Zhu ◽  
Shengyu Cui ◽  
Zhiming Cui ◽  
...  
Keyword(s):  
Side Effects ◽  
Neuronal Apoptosis ◽  
Neuronal Development ◽  
Biological Activities ◽  
Transgenic Zebrafish ◽  
Zebrafish Embryos ◽  
Nerve Growth ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Low Toxicity

Studies have shown that lobetyolin (LBT), a component of traditional Chinese herbal medicine, has many very good biological activities and functions. However, its side effects and toxicities have not been evaluated adequately. In this work, we investigated the effects of LBT in transgenic zebrafish. LBT treatments promoted angiogenesis and led to vascular morphological malformation in zebrafish embryos, although they were normal in appearance. Interestingly, our results indicated that LBT has a function of promoting nerve growth in the embryonic stage of zebrafish. We also treated the zebrafish with combretastatin A-4 (which resulted in neuronal apoptosis) and LBT simultaneously and found that LBT promoted nerve growth at higher concentrations. Taken together, our findings clearly display that LBT efficiently promotes angiogenesis, leading to vascular morphological malformation, has low toxicity, and notably promotes neuronal development in zebrafish.

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