Carbon dots-based fluorescence resonance energy transfer for the prostate specific antigen (PSA) with high sensitivity

Talanta ◽  
2020 ◽  
Vol 219 ◽  
pp. 121276 ◽  
Author(s):  
Jia Hui He ◽  
Yun Ying Cheng ◽  
Qian Qian Zhang ◽  
Hui Liu ◽  
Cheng Zhi Huang
Keyword(s):  
Energy Transfer ◽  
Prostate Specific Antigen ◽  
Fluorescence Resonance Energy Transfer ◽  
Carbon Dots ◽  
Specific Antigen ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Fluorescence Resonance

Fluorescence resonance energy transfer monitoring of pH-responsive doxorubicin release from carbon dots/aptamer functionalized magnetic mesoporous silica

Nanomedicine ◽  
2021 ◽  
Author(s):  
Abolghasem Abbasi Kajani ◽  
Masoud Ayatollahi Mehrgardi
Keyword(s):  
Drug Delivery ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Carbon Dots ◽  
Drug Loading ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Spectroscopic Techniques ◽  
Ph Responsive ◽  
Fluorescence Resonance

Aim: To develop a novel theranostic nanoplatform for simultaneous fluorescent monitoring and stimuli-triggered drug delivery. Materials & methods: Different microscopic and spectroscopic techniques were used for the characterization of nanocarriers. MCF-7 and human umbilical vein endothelial cell lines were cultured and treated with different doses of doxorubicin-loaded nanocarriers. The cell viability and drug release were studied using MTT assay and fluorescence microscopy. Results: Biocompatible and mono-disperse nanocarriers represent hollow and mesoporous structures with the calculated surface area of 552.83 m2.g-1, high magnetic activity (12.6 emu.g-1), appropriate colloidal stability and high drug loading capacity (up to 61%). Conclusion: Taxane-based carbon dots act as the pH-responsive gatekeepers for the controlled release of doxorubicin into cancer cells and provide a fluorescence resonance energy transfer system for real-time monitoring of drug delivery.

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