Effect of egg yolk plasma and soybean lecithin on rooster frozen-thawed sperm quality and fertility

2018 ◽  
Vol 116 ◽  
pp. 89-94 ◽  
Author(s):  
Mahdieh Mehdipour ◽  
Hossein Daghigh Kia ◽  
Gholamali Moghaddam ◽  
Hamed Hamishehkar
Keyword(s):  
Soybean Lecithin ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Egg Yolk Plasma ◽  
Yolk Plasma

Egg yolk plasma can replace egg yolk in stallion freezing extenders

2011 ◽  
Vol 75 (1) ◽  
pp. 105-114 ◽  
Author(s):  
E. Pillet ◽  
G. Duchamp ◽  
F. Batellier ◽  
V. Beaumal ◽  
M. Anton ◽  
...  
Keyword(s):  
Egg Yolk ◽  
Egg Yolk Plasma ◽  
Yolk Plasma

scholarly journals Spermatological Parameters of Extended Rabbit Semen in 5% Indigenous Poultry Egg Yolk Plasma-Biofortified Extender

2017 ◽  
Vol 32 (1) ◽  
pp. 91 ◽  
Author(s):  
M.A. Popoola ◽  
C.I. Alemede ◽  
A. Aremu ◽  
S.I. Ola ◽  
Y.R. Popoola ◽  
...  
Keyword(s):  
Egg Yolk ◽  
Egg Yolk Plasma ◽  
Yolk Plasma

Study on external factors affecting egg yolk plasma by asymmetrical flow field-flow fractionation

2017 ◽  
Vol 94 ◽  
pp. 13-19 ◽  
Author(s):  
Haiyang Dou ◽  
Xiaoyue Zhang ◽  
Aixia Zhang ◽  
Jaeyeong Choi ◽  
Jing Wang ◽  
...  
Keyword(s):  
Flow Field ◽  
Egg Yolk ◽  
External Factors ◽  
Field Flow Fractionation ◽  
Factors Affecting ◽  
Flow Fractionation ◽  
Egg Yolk Plasma ◽  
Yolk Plasma

Adsorption of egg yolk plasma cholesterol using a hydrophobic adsorbent

2006 ◽  
Vol 223 (5) ◽  
pp. 705-709 ◽  
Author(s):  
Edwin E. Garcia Rojas ◽  
Jane S. dos Reis Coimbra ◽  
Luis A. Minim
Keyword(s):  
Plasma Cholesterol ◽  
Egg Yolk ◽  
Egg Yolk Plasma ◽  
Yolk Plasma

Flow cytometric and microscopic evaluation of post-thawed ram semen cryopreserved in chemically defined home-made or commercial extenders

2015 ◽  
Vol 55 (4) ◽  
pp. 551 ◽  
Author(s):  
M. Emamverdi ◽  
M. Zhandi ◽  
A. Zare Shahneh ◽  
M. Sharafi ◽  
A. Akhlaghi ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Soybean Lecithin ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Mitochondrial Activity ◽  
Flow Cytometric ◽  
Microscopic Evaluation ◽  
Open Question ◽  
Ram Sperm

The present study was designed to determine the effect of three different extenders on ram sperm quality during a freeze–thawing procedure using flow cytometric and microscopic evaluations. Several in vitro qualitative analyses of post-thawed sperm parameters including motility and velocity parameters, plasma membrane functionality, total abnormality, capacitation status, acrosome integrity, mitochondrial activity and apoptosis features were considered. In the breeding season, seven ejaculates from each Zandi ram were collected routinely twice a week. Following semen collection, samples were pooled and equally divided into three aliquots. Each aliquot was diluted and frozen with one of the following extenders: (1) Tris-based extender containing 1.5% (w/v) soybean lecithin (TSL), as a chemically defined extender, (2) Bioxcell, a commercial soybean lecithin-based extender, and (3) Tris-based extender containing 20% (v/v) egg yolk (TEY). The results of the present study indicated no differences in total [TSL (55.8 ± 2.02%) vs TEY (50.2 ± 2.02%; P < 0.05)] and progressive motility of spermatozoa [TSL (26.2 ± 1.36%) vs Bioxcell (22.4 ± 1.36%; P < 0.05)]. Semen freezing by means of TSL resulted in a higher percentage of live spermatozoa (39.42 ± 1.81%) compared with TEY (29.17 ± 1.81%; P < 0.05), and a higher percentage of functional plasma membrane (50.8 ± 192%) compared with TEY (44 ± 1.92%) and Bioxcell (38.8 ± 1.92%; P < 0.05). The effect of extenders on sperm capacitation status showed that the percentage of post-thawed capacitated spermatozoa was higher in TEY (61.9 ± 1.48%) compared with that in TSL (56.6 ± 1.48%; P < 0.05). The evaluation of post-thawed spermatozoa indicated that the percentage of live spermatozoa with active mitochondria was higher in TSL (53.05 ± 2.31%) compared with Bioxcell (45.92 ± 2.31; P < 0.05) and the percentage of intact acrosome spermatozoa was higher in TSL (84.55 ± 2.51%) compared with TEY (74.91 ± 2.51%; P < 0.05). The use of TSL and Bioxcell extenders reduced the percentage of apoptotic spermatozoa (40.82 ± 2.07% and 42.22 ± 2.07%, respectively), compared with TEY (51.34 ± 2.07%; P < 0.05). Post-thawing dead spermatozoa were increased when semen was frozen by Bioxcell (25.69 ± 1.28%). The results of this study showed that TSL extender may provide stabile milieu and conditions for ram sperm cryopreservation compared with Bioxcell and TEY extenders. Whether TSL extender can improve the artificial insemination results remains, however, an open question.

scholarly journals Effects of Egg Yolk and Egg Yolk Plasma Tris-Base Extenders on Beetal Buck Semen Preservation

2020 ◽  
Vol 9 (11) ◽  
pp. 1853-1864
Author(s):  
Bipanpreet Singh ◽  
Daljeet Kaur ◽  
Mandeep Singla ◽  
Ranjna S. Cheema ◽  
A.P.S. Sethi ◽  
...  
Keyword(s):  
Egg Yolk ◽  
Semen Preservation ◽  
Egg Yolk Plasma ◽  
Yolk Plasma
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